+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Genome-wide DArT and SNP scan for QTL associated with resistance to stripe rust (Puccinia striiformis f. sp. tritici) in elite ICARDA wheat (Triticum aestivum L.) germplasm



Genome-wide DArT and SNP scan for QTL associated with resistance to stripe rust (Puccinia striiformis f. sp. tritici) in elite ICARDA wheat (Triticum aestivum L.) germplasm



Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 128(7): 1277-1295



Identified DArT and SNP markers including a first reported QTL on 3AS, validated large effect APR on 3BS. The different genes can be used to incorporate stripe resistance in cultivated varieties. Stripe rust [yellow rust, caused by Puccinia striiformis f. sp. tritici (Pst)] is a serious disease in wheat (Triticum aestivum). This study employed genome-wide association mapping (GWAM) to identify markers linked to stripe rust resistance genes using Diversity Arrays Technology (DArT(®)) and single-nucleotide polymorphism (SNP) Infinium 9K assays in 200 ICARDA wheat genotypes, phenotyped for seedling and adult plant resistance in two sites over two growing seasons in Syria. Only 25.8 % of the genotypes showed resistance at seedling stage while about 33 and 44 % showed moderate resistance and resistance response, respectively. Mixed-linear model adjusted for false discovery rate at p < 0.05 identified 12 DArT and 29 SNP markers on chromosome arms 3AS, 3AL, 1AL, 2AL, 2BS, 2BL, 3BS, 3BL, 5BL, 6AL, and 7DS significantly linked to Pst resistance genes. Of these, the locus on 3AS has not been previously reported to confer resistance to stripe rust in wheat. The QTL on 3AS, 3AL, 1AL, 2AL, and 2BS were effective at seedling and adult plant growth stages while those on 3BS, 3BL, 5BL, 6AL and 7DS were effective at adult plant stage. The 3BS QTL was validated in Cham-6 × Cham-8 recombinant inbred line population; composite interval analysis identified a stripe resistance QTL flanked by the DArT marker, wPt-798970, contributed by Cham-6 parent which accounted for 31.2 % of the phenotypic variation. The DArT marker "wPt-798970" lies 1.6 cM away from the 3BS QTL detected within GWAM. Epistatic interactions were also investigated; only the QTL on 1AL, 3AS and 6AL exhibited interactions with other loci. These results suggest that GWAM can be an effective approach for identifying and improving resistance to stripe rust in wheat.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 057935041

Download citation: RISBibTeXText

PMID: 25851000

DOI: 10.1007/s00122-015-2504-2


Related references

A genome-wide association study of resistance to stripe rust (Puccinia striiformis f. sp. tritici) in a worldwide collection of hexaploid spring wheat (Triticum aestivum L.). G3 5(3): 449-465, 2015

Genome-wide association mapping for stripe rust (Puccinia striiformis F. sp. tritici) in US Pacific Northwest winter wheat (Triticum aestivum L.). Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 128(6): 1083-1101, 2015

Inheritance of seedling resistance to stripe rust (Puccinia striiformis f. sp. tritici) in 'Otane' and 'Tiritea' wheat (Triticum aestivum). New Zealand Journal of Crop and Horticultural Science 31(1): 15-22, 2003

Loci associated with resistance to stripe rust (Puccinia striiformis f. sp. tritici) in a core collection of spring wheat (Triticum aestivum). Plos one 12(6): E0179087, 2017

Inheritance of durable adult plant resistance to stripe rust (Puccinia striiformis f. sp tritici) in 'Otane' wheat (Triticum aestivum). New Zealand Journal of Crop and Horticultural Science 31(1): 23-31, 2003

Genome-wide association study of resistance to stripe rust (Puccinia striiformis f. sp. tritici) in Sichuan wheat. Bmc Plant Biology 19(1): 147, 2019

Identifying QTL for high-temperature adult-plant resistance to stripe rust (Puccinia striiformis f. sp. tritici) in the spring wheat (Triticum aestivum L.) cultivar 'Louise'. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 119(6): 1119-1128, 2009

Fungicide evaluation for control of wheat Septoria blotchs and rusts, 1981 Wheat (Triticum aestivum Stephens, Yamhill, Omar), stripe rust; Puccinia striiformis, leaf rust; Puccinia recondita f.sp. tritici, Septoria blotch; Septoria tritici and Septoria nodorum. Fungicide and nematicide tests results American Phytopathological Society7(37): 122, 1982

Genome-wide identification of QTL conferring high-temperature adult-plant HTAP resistance to stripe rust Puccinia striiformis f sp tritici in wheat. Molecular Breeding 29(3): 791-800, 2012

Suppression of take-all root rot and stripe rust diseases of wheat with chloride fertilizers Gaeumannomyces graminis tritici, Puccinia striiformis, Triticum aestivum. Plant nutrition 1982: proceedings of the ninth International Plant Nutrition Colloquium Warwick University England August 22-27-1982 edited by A Scaife: 116, 1982

Genetic behavior of controlling area under disease progress curve for stripe rust (Puccinia striiformis f. sp. tritici) in two wheat (Triticum aestivum) crosses. Phytopathology 99(11): 1265-1272, 2009

Inheritance of resistance to stripe rust puccinia striiformis in some yellow rust resistance testers of wheat triticum aestivum. Indian Journal of Agricultural Sciences 59(10): 617-621, 1989

Genome Sequence Resources for the Wheat Stripe Rust Pathogen (Puccinia striiformis f. sp. tritici) and the Barley Stripe Rust Pathogen (Puccinia striiformis f. sp. hordei). Molecular Plant-Microbe Interactions 31(11): 1117-1120, 2018

Development of additive resistance in wheat, Triticum aestivum L., to stripe rust, Puccinia striiformis West. Dissertation Abstracts International, B 38(6): 2457B, 1977

Influence of stripe rust (Puccinia striiformis) on three levels of resistance in wheat Triticum aestivum Vill., Host. Crop Sci. Abst, 17, 1966