+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

IDH1R¹³²H decreases the proliferation of U87 glioma cells through upregulation of microRNA-128a



IDH1R¹³²H decreases the proliferation of U87 glioma cells through upregulation of microRNA-128a



Molecular Medicine Reports 12(5): 6695-6701



Mutations in isocitrate dehydrogenase 1 (IDH1) are found in >70% of secondary glioblastomas and lower-grade gliomas (grades II-III). Among the numerous phenotypic differences between IDH1 mutant and wild-type glioma patients, the most salient is an improved survival rate for patients with a mutation. MicroRNAs (miRNAs) are a class of small, non‑coding, single‑stranded RNAs that can negatively regulate gene expression at the post‑transcriptional level, predominantly by binding to the 3'‑untranslated region of their target mRNAs. The dysregulated expression of several miRNAs has been reported to modulate glioma progression; however, it is unclear whether mutations in IDH1 regulate glioma cell proliferation through miRNA dysregulation. In the present study, stable overexpression of IDH1WT or IDH1R132H was established in the U87 glioma cell line. It was found that IDH1R132H decreased cell proliferation of U87 glioma cells by inducing the expression of the miRNA miR‑128a. This process was dependent on the transcription factor hypoxia inducible factor‑1α (HIF‑1α), which binds to a hypoxia response element in the promoter of miR‑128a. Furthermore, miR‑128a negatively regulated the expression of B‑cell‑specific Moloney murine leukemia virus integration site 1 protein (Bmi‑1), which is involved in suppressing cell proliferation. These findings suggest that the IDH1R132H‑HIF‑1α‑miR‑128a‑Bmi‑1 pathway is involved in glioma cell proliferation.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 058030020

Download citation: RISBibTeXText

PMID: 26324126

DOI: 10.3892/mmr.2015.4241


Related references

Effects of mir-128a on the invasion and proliferation of glioma U251 cells. Oncology Letters 17(1): 891-896, 2019

Osthole suppresses the proliferation and accelerates the apoptosis of human glioma cells via the upregulation of microRNA-16 and downregulation of MMP-9. Molecular Medicine Reports 12(3): 4592-4597, 2015

Inhibition of the Proliferation and Invasion of C6 Glioma Cells by Tricin via the Upregulation of Focal-Adhesion-Kinase-Targeting MicroRNA-7. Journal of Agricultural and Food Chemistry 66(26): 6708-6716, 2018

Paeoniflorin inhibits proliferation and induces apoptosis of human glioma cells via microRNA-16 upregulation and matrix metalloproteinase-9 downregulation. Molecular Medicine Reports 12(2): 2735-2740, 2015

MicroRNA-29a-3p Downregulation Causes Gab1 Upregulation to Promote Glioma Cell Proliferation. Cellular Physiology and Biochemistry 48(2): 450-460, 2018

Effects of microRNA-195 on the Prognosis of Glioma Patients and the Proliferation and Apoptosis of Human Glioma Cells. Pathology Oncology Research 2019:, 2019

OS2.6Identification of IDH1R132H-specific T cell receptors from a humanized mouse model and from glioma patients treated with an IDH1R132H-specific peptide vaccine. Neuro-Oncology 18(Suppl_4): Iv5-Iv6, 2016

O4.08 * Agi5198, An Inhibitor Of Idh1R132H, Protects Idh1R132H Hct116 Cells Against Irradiation. Neuro-Oncology 16(Suppl 2): ii8-ii9, 2014

Co-inhibition of microRNA-10b and microRNA-21 exerts synergistic inhibition on the proliferation and invasion of human glioma cells. International Journal of Oncology 41(3): 1005-1012, 2012

MicroRNA-128a-induced apoptosis in HTR-8/SVneo trophoblast cells contributes to pre-eclampsia. Biomedicine and PharmacoTherapy 81: 63-70, 2016

Spontaneous in vitro senescence of glioma cells confirmed by an antibody against IDH1R132H. Anticancer Research 34(6): 2859-2867, 2014

Histone deacetylase 5 promotes the proliferation of glioma cells by upregulation of Notch 1. Molecular Medicine Reports 10(4): 2045-2050, 2014

Upregulation of microRNA-206 induces apoptosis of vascular smooth muscle cells and decreases risk of atherosclerosis through modulating FOXP1. Experimental and Therapeutic Medicine 14(5): 4097-4103, 2017

The role of microRNA-128a in regulating TGFbeta signaling in letrozole-resistant breast cancer cells. Breast Cancer Research and Treatment 124(1): 89-99, 2010

Upregulation of microRNA-383 inhibits the proliferation, migration and invasion of colon cancer cells. Oncology Letters 15(1): 1184-1190, 2018