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Identification of Protein Kinase Substrates by the Kinase-Interacting Substrate Screening (KISS) Approach



Identification of Protein Kinase Substrates by the Kinase-Interacting Substrate Screening (KISS) Approach



Current Protocols in Cell Biology 72: 14.16.1-14.16.12



Identifying the substrates of protein kinases to understand their modes of action has been undertaken by various approaches and remains an ongoing challenge. Phosphoproteomic technologies have accelerated the accumulation of data concerning protein phosphorylation and have uncovered vast numbers of phosphorylation sites in vivo. In this unit, a novel in vitro screening approach for protein kinase substrates is presented, based on protein-protein interaction and mass spectrometry-based phosphoproteomic technology. © 2016 by John Wiley & Sons, Inc.

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Accession: 058036992

Download citation: RISBibTeXText

PMID: 27580705

DOI: 10.1002/cpcb.8


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