+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Identification of a Novel Small Molecule Inhibitor Against SARS Coronavirus Helicase

Identification of a Novel Small Molecule Inhibitor Against SARS Coronavirus Helicase

Journal of Microbiology and Biotechnology 25(12): 2007-2010

A new chemical inhibitor against severe acute respiratory syndrome (SARS) coronavirus helicase, 7-ethyl-8-mercapto-3-methyl-3,7-dihydro-1H-purine-2,6-dione, was identified. We investigated the inhibitory effect of the compound by conducting colorimetry-based ATP hydrolysis assay and fluorescence resonance energy transfer-based double-stranded DNA unwinding assay. The compound suppressed both ATP hydrolysis and double-stranded DNA unwinding activities of helicase with IC50 values of 8.66 ± 0.26 μM and 41.6 ± 2.3 μM, respectively. Moreover, we observed that the compound did not show cytotoxicity up to 80 µMconcentration. Our results suggest that the compound might serve as a SARS coronavirus inhibitor.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 058037698

Download citation: RISBibTeXText

PMID: 26387819

Related references

Identification of a small molecule PriA helicase inhibitor. Biochemistry 51(51): 10137-10146, 2012

Identification of myricetin and scutellarein as novel chemical inhibitors of the SARS coronavirus helicase, nsP13. Bioorganic and Medicinal Chemistry Letters 22(12): 4049-4054, 2012

From genome to drug lead: identification of a small-molecule inhibitor of the SARS virus. Bioorganic and Medicinal Chemistry Letters 16(4): 830-833, 2006

Differential sensitivities of severe acute respiratory syndrome (SARS) coronavirus spike polypeptide enzyme-linked immunosorbent assay (ELISA) and SARS coronavirus nucleocapsid protein ELISA for serodiagnosis of SARS coronavirus pneumonia. Journal of Clinical Microbiology 43(7): 3054-3058, 2005

Design and synthesis of a series of serine derivatives as small molecule inhibitors of the SARS coronavirus 3CL protease. Bioorganic and Medicinal Chemistry 24(6): 1241-1254, 2016

An Overview of Severe Acute Respiratory Syndrome-Coronavirus (SARS-CoV) 3CL Protease Inhibitors: Peptidomimetics and Small Molecule Chemotherapy. Journal of Medicinal Chemistry 59(14): 6595-6628, 2016

Substrate specificity profiling and identification of a new class of inhibitor for the major protease of the SARS coronavirus. Biochemistry 46(30): 8744-8752, 2007

Inhibition of SARS coronavirus helicase by bismuth complexes. Chemical Communications 2007(42): 4413-4415, 2007

Tertiary structure prediction of SARS coronavirus helicase. Biochemical and Biophysical Research Communications 343(4): 1101-1104, 2006

A Novel Chemical Compound for Inhibition of SARS Coronavirus Helicase. Journal of Microbiology and Biotechnology 27(11): 2070-2073, 2017

Differential inhibitory activities and stabilisation of DNA aptamers against the SARS coronavirus helicase. Chembiochem 9(18): 3037-3045, 2008

Cooperative translocation enhances the unwinding of duplex DNA by SARS coronavirus helicase nsP13. Nucleic Acids Research 38(21): 7626-7636, 2010

Development of chemical inhibitors of the SARS coronavirus: viral helicase as a potential target. Biochemical Pharmacology 84(10): 1351-1358, 2012

The SARS-coronavirus-host interactome: identification of cyclophilins as target for pan-coronavirus inhibitors. Plos Pathogens 7(10): E1002331, 2011

Identification of pulmonary Oct-4+ stem/progenitor cells and demonstration of their susceptibility to SARS coronavirus (SARS-CoV) infection in vitro. Proceedings of the National Academy of Sciences of the United States of America 103(25): 9530-9535, 2006