+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Identification of a plasma four-microRNA panel as potential noninvasive biomarker for osteosarcoma

Identification of a plasma four-microRNA panel as potential noninvasive biomarker for osteosarcoma

Plos One 10(3): E0121499

Circulating microRNAs (miRNAs) are emerging as promising biomarkers for human cancer. Osteosarcoma is the most common human primary malignant bone tumor in children and young adults. The objective of this study was to investigate whether circulating miRNAs in plasma could be a useful biomarker for detecting osteosarcoma and monitoring tumor removal dynamics. Plasma samples were obtained from 90 patients before surgery, 50 patients after one month of surgery, and 90 healthy individuals. The study was divided into three steps: First, initial screening of the profiles of circulating miRNAs in pooled plasma samples from healthy controls and pre-operative osteosarcoma patients using a TaqMan low density array (TLDA). Second, evaluation of miRNA concentration in individual plasma samples from 90 pre-operative osteosarcoma patients and 90 healthy controls by a quantitative real time PCR (qRT-PCR) assay. Third, evaluation of miRNA concentration in paired plasma samples from 50 pre- and post-operative osteosarcoma patients by qRT-PCR assay. Four plasma miRNAs including miR-195-5p, miR-199a-3p, miR-320a, and miR-374a-5p were significantly increased in the osteosarcoma patients. Receiver operating characteristics curve analysis of the combined populations demonstrated that the four-miRNA signature could discriminate cases from controls with an area under the curve of 0.9608 (95% CI 0.9307-0.9912). These 4 miRNAs were markedly decreased in the plasma after operation. In addition, circulating miR-195-5p and miR-199a-3p were correlated with metastasis status, while miR-199a-3p and miR-320a were correlated with histological subtype. Our data suggest that altered levels of circulating miRNAs might have great potential to serve as novel, non-invasive biomarkers for osteosarcoma.

(PDF emailed within 1 workday: $29.90)

Accession: 058038292

Download citation: RISBibTeXText

PMID: 25775010

Related references

A six-microRNA panel in plasma was identified as a potential biomarker for lung adenocarcinoma diagnosis. Oncotarget 8(4): 6513-6525, 2016

Identification of microRNA-135b in stool as a potential noninvasive biomarker for colorectal cancer and adenoma. Clinical Cancer Research 20(11): 2994-3002, 2015

A four serum-miRNA panel serves as a potential diagnostic biomarker of osteosarcoma. International Journal of Clinical Oncology 2019, 2019

Identification of Serum MicroRNA-21 as a Biomarker for Chemosensitivity and Prognosis in Human Osteosarcoma. Journal of International Medical Research 40(6): 2090-2097, 2012

MicroRNA-375 as a potential serum biomarker for the diagnosis, prognosis, and chemosensitivity prediction of osteosarcoma. Journal of International Medical Research 46(3): 975-983, 2017

Increased expression of microRNA-191 as a potential serum biomarker for diagnosis and prognosis in human osteosarcoma. Cancer Biomarkers 15(5): 543-550, 2016

A serum exosomal microRNA panel as a potential biomarker test for gastric cancer. Biochemical and Biophysical Research Communications 493(3): 1322-1328, 2017

Plasma microRNA signature as a noninvasive biomarker for acute graft-versus-host disease. Blood 122(19): 3365-3375, 2014

Serum microRNA panel excavated by machine learning as a potential biomarker for the detection of gastric cancer. Oncology Reports 39(3): 1338-1346, 2017

Urinary and Blood MicroRNA-126 and -770 are Potential Noninvasive Biomarker Candidates for Diabetic Nephropathy: a Meta-Analysis. Cellular Physiology and Biochemistry 46(4): 1331-1340, 2018

Identification of circulating miR-663a as a potential biomarker for diagnosing osteosarcoma. Pathology, Research and Practice 2019, 2019

Usefulness of Plasma Exosomal MicroRNA-451a as a Noninvasive Biomarker for Early Prediction of Recurrence and Prognosis of Non-Small Cell Lung Cancer. Oncology 94(5): 311-323, 2018

Identification of plasma miR-148a as a noninvasive biomarker for hepatocellular carcinoma. Clinics and Research in Hepatology and Gastroenterology 2019, 2019

Plasma microRNA-21 is a potential diagnostic biomarker of acute myocardial infarction. European Review for Medical and Pharmacological Sciences 20(2): 323-329, 2016

MicroRNA-134 as a potential plasma biomarker for the diagnosis of acute pulmonary embolism. Journal of Translational Medicine 9: 159, 2012