+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Identification of chemical constituents and rat metabolites of Kangxianling granule by HPLC-Q-TOF-MS/MS



Identification of chemical constituents and rat metabolites of Kangxianling granule by HPLC-Q-TOF-MS/MS



Biomedical Chromatography 29(11): 1750-1758



A high-performance liquid chromatography coupled with quadrupole time-of-flight mass tandem mass spectrometry method was established to characterize the chemical constituents of Kangxianling granule (KXL), a traditional Chinese medicine formula, and the metabolic profile in rat urine and plasma after oral administration of KXL. A total of 27 compounds in KXL extract and 13 prototype compounds with 12 metabolites in rat urine and plasma were identified. Among the 27 detected compounds, 15 were identified by comparing the retention time and MS data with that of reference compounds and the other 12 compounds were tentatively assigned based on the MS data and reference literature. The main prototype components absorbed in rat were amygdalin, salvianolic acid B, tanshinones and anthraquinones. Hydroxylation, glucuronidation and sulfation were the principal metabolic pathways in rat. The results revealed that the 25 compounds identified in rat urine and plasma were the potential active ingredients of KXL, which provides helpful chemical information for further study of the pharmacology mechanism of KXL.

(PDF emailed within 0-6 h: $19.90)

Accession: 058038869

Download citation: RISBibTeXText

PMID: 25990409

DOI: 10.1002/bmc.3489


Related references

The profiling and identification of the absorbed constituents and metabolites of Paeoniae Radix Rubra decoction in rat plasma and urine by the HPLC-DAD-ESI-IT-TOF-MS(n) technique: a novel strategy for the systematic screening and identification of absorbed constituents and metabolites from traditional Chinese medicines. Journal of Pharmaceutical and Biomedical Analysis 83: 108-121, 2014

Identification and analysis of chemical constituents and rat serum metabolites in Suan-Zao-Ren granule using ultra high performance liquid chromatography quadrupole time-of-flight mass spectrometry combined with multiple data processing approaches. Journal of Separation Science 40(14): 2914-2924, 2017

Investigation of potential toxic components based on the identification of Genkwa Flos chemical constituents and their metabolites by HPLC coupled with a Q Exactive high-resolution benchtop quadrupole Orbitrap mass spectrometer. Journal of Separation Science, 2018

Rapid characterization of chemical constituents and metabolites of Qi-Jing-Sheng-Bai granule by using UHPLC-Q-TOF-MS. Journal of Separation Science, 2018

Fingerprint analysis of Gelsemium elegans by HPLC followed by the targeted identification of chemical constituents using HPLC coupled with quadrupole-time-of-flight mass spectrometry. Fitoterapia 121: 94-105, 2017

Characterization of chemical constituents and rats metabolites of Shuanghua Baihe tablets by HPLC-Q-TOF-MS/MS. Biomedical Chromatography 29(1): 75-86, 2015

Identification of chemical constituents in Yangxue Qingnao granule by UPLC-Q-TOF/MS~E. Yao Xue Xue Bao 51(5): 797-805, 2018

The profiling and identification of the absorbed constituents and metabolites of Paeoniae Radix Rubra decoction in rat plasma and urine by the HPLC-DAD-ESI-IT-TOF-MSn technique A novel strategy for the systematic screening and identification of absorbed. 2013

Identification of chemical constituents in Scrophulariae Radix by HPLC-IT-TOF-MS. Zhongguo Zhong Yao Za Zhi 41(7): 1257-1268, 2016

Identification of chemical constituents in Sinopodophylli Fructus by HPLC-DAD-ESI-IT-TOF-MSn. Zhongguo Zhong Yao Za Zhi 43(1): 123-133, 2018

HPLC/Q-TOF-MS-Based Identification of Absorbed Constituents and Their Metabolites in Rat Serum and Urine after Oral Administration of Cistanche deserticola Extract. Journal of Food Science 80(9): H2079-H2087, 2016

Rapid identification of chemical constituents in Salvia chinensis by HPLC-TOF-MS. Zhongguo Zhong Yao Za Zhi 38(23): 4109-4112, 2014

Identification of chemical constituents of Yuanhu Zhitong prescription by HPLC-QTOF/MS. Yao Xue Xue Bao 52(1): 132-138, 2018

Identification of the absorptive constituents and their metabolites in vivo of Puerariae Lobatae Radix decoction orally administered in WZS-miniature pigs by HPLC-ESI-Q-TOFMS. Biomedical Chromatography 27(9): 1208-1218, 2013

Identification of the polar constituents of Potamogeton species by HPLC-UV with post-column derivatization, HPLC-MSn and HPLC-NMR, and isolation of a new ent-labdane diglycoside. Phytochemistry 65(16): 2401-2410, 2004