+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Identification of differentially expressed genes in the spleens of polyriboinosinic polyribocytidylic acid (poly I:C)-stimulated yellow catfish Pelteobagrus fulvidraco

Identification of differentially expressed genes in the spleens of polyriboinosinic polyribocytidylic acid (poly I:C)-stimulated yellow catfish Pelteobagrus fulvidraco

Fish and Shellfish Immunology 56: 278-285

The yellow catfish, Pelteobagrus fulvidraco (Siluriformes: Bagridae) is an economically important fish in China. However, genomic research and resources on this species are largely unavailable and still in infancy. In the present study, we constructed a cDNA library following poly I:C injection to screen for immune response genes in the spleens of P. fulvidraco using suppression subtractive hybridization (SSH). A total of 420 putative expressed sequence tag (EST) clones were identified at 24 h post-injection, which contain 103 genes consisting of 25 immune response genes, 12 cytoskeleton genes, 7 cell cycle and apoptosis genes, 7 respiration and energy metabolism genes, 7 transport genes, 26 metabolism genes, 10 stress response genes, 9 translational regulation genes, and 71 unknown genes. Real-time quantitative reverse transcription-PCR (qRT-PCR) results revealed that a set of randomly selected immune response genes were identified to be up-regulated after 24 h of poly I:C stimulation compared to controls. Our study provides an annotation of immune genes in detail and insight into fish immunity.

(PDF emailed within 0-6 h: $19.90)

Accession: 058039084

Download citation: RISBibTeXText

PMID: 27368543

DOI: 10.1016/j.fsi.2016.06.041

Related references

Transcriptome analysis of yellow catfish (Pelteobagrus fulvidraco) liver challenged with polyriboinosinic polyribocytidylic acid (poly I:C). Fish and Shellfish Immunology 68: 395-403, 2017

A myeloid differentiation factor 88 gene from yellow catfish Pelteobagrus fulvidraco and its molecular characterization in response to polyriboinosinic polyribocytidylic acid and lipopolysaccharide challenge. International Journal of Biological Macromolecules 120(Pt A): 1080-1086, 2018

Gonadal transcriptomic analysis of yellow catfish (Pelteobagrus fulvidraco): identification of sex-related genes and genetic markers. Physiological Genomics 46(21): 798-807, 2015

RAPD markers between yellow catfish Pelteobagrus fulvidraco and long yellow catfish Pelteobagrus eupogon. 2006

Identification of five genes in endoplasmic reticulum (ER) stress-apoptosis pathways in yellow catfish Pelteobagrus fulvidraco and their transcriptional responses to dietary lipid levels. Fish Physiology and Biochemistry 2019, 2019

Effect of treatment with exogenous interferon, polyriboinosinic-polyribocytidylic acid, or polyriboinosinic-polyribocytidylic acid-poly-L-lysine complex on Herpesvirus hominis infections in mice. Journal of Infectious Diseases 137(4): 428-436, 1978

Three unsaturated fatty acid biosynthesis-related genes in yellow catfish Pelteobagrus fulvidraco: Molecular characterization, tissue expression and transcriptional regulation by leptin. Gene 563(1): 1-9, 2015

Characterization of immune-related genes in the yellow catfish Pelteobagrus fulvidraco in response to LPS challenge. Fish and Shellfish Immunology 56: 248-254, 2017

Sex differences in the expression of GH/IGF axis genes underlie sexual size dimorphism in the yellow catfish (Pelteobagrus fulvidraco). Science China. Life Sciences 59(4): 431-433, 2017

Molecular cloning and expression pattern of 11 genes involved in lipid metabolism in yellow catfish Pelteobagrus fulvidraco. Gene 531(1): 53-63, 2013

Transcriptomic Analyses Reveal Novel Genes with Sexually Dimorphic Expression in Yellow Catfish (Pelteobagrus fulvidraco) Brain. Marine Biotechnology 17(5): 613-623, 2016

Isolation and identification of pelteobagrin, a novel antimicrobial peptide from the skin mucus of yellow catfish (Pelteobagrus fulvidraco). Comparative Biochemistry and Physiology. Part B, Biochemistry and Molecular Biology 158(2): 149-154, 2011

Astragalus polysaccharides, chitosan and poly(I:C) obviously enhance inactivated Edwardsiella ictaluri vaccine potency in yellow catfish Pelteobagrus fulvidraco. Fish & Shellfish Immunology 2019, 2019

RAPD (random amplification of polymorphic DNA) markers between yellow catfish (Pelteobagrus fulvidraco) and long whiskers yellow catfish (P. eupogon). 2006

Potential contributions of heat shock proteins and related genes in sexual differentiation in yellow catfish (Pelteobagrus fulvidraco). Fish Physiology and Biochemistry 43(2): 465-475, 2017