+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Identification of differentially expressed three novel transcript variants of mouse ARNT gene

Identification of differentially expressed three novel transcript variants of mouse ARNT gene

Iubmb Life 68(2): 122-135

The aryl hydrocarbon receptor nuclear translocator (ARNT/HIF1-β) is an obligatory transcriptional partner of the aryl hydrocarbon receptor (AHR) and hypoxia-inducible factor-1α (HIF-1α). It has a basic helix-loop-helix domain that belongs to period-ARNT-single-minded (PAS) protein family. PAS proteins act as heterodimeric transcription factors with ARNT being master dimerization partner. The ARNT-HIF-1α complex is an important transcriptional regulator of the hypoxic response of the tumor cells. Previous studies have reported two transcript variants of the gene produced by alternative splicing in mouse. One transcript variant contains all 22 exons while the other variant lacks exon-E5. In our study, using combinatorial approach comprising bioinformatics tools and molecular biology techniques involving RT-PCR, semi-nested PCR, sequencing and qPCR, we have identified three novel transcript variants of Arnt gene in mouse. All three new transcripts arise as a result of alternative splicing of newly identified exons with exon-E2, replacing reported exon-E1. These transcripts encode for three protein isofoms having different N-termini. The expression of these transcripts was found to be different in different tissues of adult mice. In silico analysis of the upstream region of the new exons revealed three distinct promoter regions designated as PA, PB and PC present upstream of newly identified exons. These promoters possess potential signature sequences for common as well as different transcription factors suggesting complex regulation of Arnt gene. In silico post translational studies of the conceptually translated amino acid sequences of these transcripts show similarity in some of the properties while differ in others. The diversity at N-termini of protein isoforms suggests the possibility of forming different complexes in different tissues and may also be important for unique interactions with partner molecules.

(PDF emailed within 0-6 h: $19.90)

Accession: 058039101

Download citation: RISBibTeXText

PMID: 26707719

DOI: 10.1002/iub.1464

Related references

Identification of multiple alternative splice variants of the KOR-3/ORL-1 receptor gene that are expressed differentially in mouse brain. Society for Neuroscience Abstracts 24(1-2): 1595, 1998

Identification, characterization, and functional analysis of sp1 transcript variants expressed in germ cells during mouse spermatogenesis. Biology of Reproduction 72(4): 898-907, 2004

The tumor microenvironment modulates endothelial cells gene expression Identification of a differentially expressed transcript. Haemostasis 31(Suppl 1): 83, November, 2001

Splice variants of the mouse Tec gene are differentially expressed in vivo. Cytogenetics and Cell Genetics 84(1-2): 132-139, 1999

Identification of a novel cytokine-like transcript differentially expressed in avian gammadelta T cells. Immunogenetics 55(12): 845-854, 2004

Identification of a differentially expressed transcript in endothelial cells exposed to tumor microenvironment. Tumori 87(6): S26, November-December, 2001

Identification of differentially expressed genes in scrapie-infected mouse brains by using global gene expression technology. Journal of Virology 78(20): 11051-11060, 2004

Identification of differentially expressed sense and antisense transcript pairs in breast epithelial tissues. Bmc Genomics 10: 324, 2009

Identification of a differentially expressed transcript in the dorsal root ganglia of galanin knockout mice. Society for Neuroscience Abstracts 27(1): 142, 2001

Sugarcane genes differentially expressed in response to Puccinia melanocephala infection: identification and transcript profiling. Plant Cell Reports 31(5): 955-969, 2012

Identification and characterisation of a polyubiquitin transcript differentially expressed in rat and human vascular smooth muscle cells. Clinical Science (London) 89(1): 5P, 1995

Identification of differentially expressed genes involved in transient regeneration of the neonatal C57BL/6J mouse heart by digital gene expression profiling. Molecular Medicine Reports 9(6): 2111-2116, 2015

Identification of differentially expressed genes between fetal and adult mouse kidney: candidate gene in kidney development. Nephron. Physiology 102(3-4): P81-P91, 2005

Identification of differentially expressed 5'-end mRNA variants by an improved RACE technique (PEETA). Nucleic Acids Research 27(15): E8, 1999

Identification of a new transcript specifically expressed in mouse spermatocytes: mmrp2. Molecular Biology Reports 32(4): 247-255, 2005