+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Identification of four plastid-localized protein kinases

Identification of four plastid-localized protein kinases

Febs Letters 590(12): 1749-1756

In chloroplasts, protein phosphorylation regulates important processes, including metabolism, photosynthesis, gene expression, and signaling. Because the hitherto known plastid protein kinases represent only a fraction of existing kinases, we aimed at the identification of novel plastid-localized protein kinases that potentially phosphorylate enzymes of the tetrapyrrole biosynthesis (TBS) pathway. We screened publicly available databases for proteins annotated as putative protein kinase family proteins with predicted chloroplast localization. Additionally, we analyzed chloroplast fractions which were separated by sucrose density gradient centrifugation by mass spectrometry. We identified four new candidates for protein kinases, which were confirmed to be plastid localized by expression of GFP-fusion proteins in tobacco leaves. A phosphorylation assay with the purified kinases confirmed the protein kinase activity for two of them.

(PDF emailed within 0-6 h: $19.90)

Accession: 058039306

Download citation: RISBibTeXText

PMID: 27214872

DOI: 10.1002/1873-3468.12223

Related references

The plastid-localized pfkB-type carbohydrate kinases FRUCTOKINASE-LIKE 1 and 2 are essential for growth and development of Arabidopsis thaliana. Bmc Plant Biology 12: 102, 2012

Chromoplast development in ripening tomato fruit: identification of cDNAs for chromoplast-targeted proteins and characterization of a cDNA encoding a plastid-localized low-molecular-weight heat shock protein. Plant Molecular Biology 33(3): 483-492, 1997

Identification of 2 subclasses of type ii cyclic amp dependent protein kinases neural specific and nonneural protein kinases. Journal of Biological Chemistry 255(17): 8179-8184, 1980

Protein kinases from spinach chloroplasts. I. Purification and identification of two distinct protein kinases. Journal of Biological Chemistry 257(20): 12153-6, 1982

Protein kinases from spinach chloroplasts 1. purification and identification of 2 distinct protein kinases. Journal of Biological Chemistry 257(20): 12153-12156, 1982

Protein Kinases and Phosphatases of the Plastid and Their Potential Role in Starch Metabolism. Frontiers in Plant Science 9: 1032, 2018

Mutations in a plastid-localized elongation factor G alter early stages of plastid development in Arabidopsis thaliana. Bmc Plant Biology 7: 37, 2007

A chloroplast-localized PPR protein required for plastid ribosome accumulation. Plant journal 36(5): 675-686, 2003

Molecular identification and characterization of a family of kinases with homology to Ca2+/calmodulin-dependent protein kinases I/IV. Journal of Biological Chemistry 281(29): 20427-20439, 2006

Identification of a fungi-specific lineage of protein kinases closely related to tyrosine kinases. Plos One 9(2): E89813, 2014

Regulation of plastid gene expression in rice involves calcium and protein phosphatases/kinases for signal transduction. Plant Science (Shannon) 137(2): 185-190, Oct 9, 1998

GUN1 Controls Accumulation of the Plastid Ribosomal Protein S1 at the Protein Level and Interacts with Proteins Involved in Plastid Protein Homeostasis. Plant Physiology 170(3): 1817-1830, 2016

A Plastid-Localized Pentatricopeptide Repeat Protein is Required for Both Pollen Development and Plant Growth in Rice. Scientific Reports 7: 11484, 2017

A wheat (Triticum aestivum) cDNA clone encoding a plastid-localized heat-shock protein. Plant Physiology 103(2): 675-676, 1993

Cloning, sequence analysis, and expression of a cDNA encoding a plastid-localized heat shock protein in maize. Plant Physiology 93(4): 1321-1328, 1990