+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Identification of fruit related microRNAs in cucumber (Cucumis sativus L.) using high-throughput sequencing technology



Identification of fruit related microRNAs in cucumber (Cucumis sativus L.) using high-throughput sequencing technology



Hereditas 151(6): 220-228



MicroRNAs (miRNAs) are approximately 21 nt noncoding RNAs that influence the phenotypes of different species through the post-transcriptional regulation of gene expression. Although many miRNAs have been identified in a few model plants, less is known about miRNAs specific to cucumber (Cucumis sativus L.). In this study, two libraries of cucumber RNA, one based on fruit samples and another based on mixed samples from leaves, stems, and roots, were prepared for deep-sequencing. A total of 110 sequences were matched to known miRNAs in 47 families, while 56 sequences in 46 families are newly identified in cucumber. Of these, 77 known and 44 new miRNAs were differentially expressed, with a fold-change of at least 2 and p-value < 0.05. In addition, we predicted the potential targets of known and new miRNAs. The identification and characterization of known and new miRNAs will enable us to better understand the role of these miRNAs in the formation of cucumber fruit.

(PDF emailed within 0-6 h: $19.90)

Accession: 058039312

Download citation: RISBibTeXText

PMID: 25588308

DOI: 10.1111/hrd2.00057


Related references

Identification of anthocyanin biosynthesis related microRNAs in a distinctive Chinese radish (Raphanus sativus L.) by high-throughput sequencing. Molecular Genetics and Genomics, 2016

A High-Density Genetic Linkage Map for Cucumber (Cucumis sativus L.): Based on Specific Length Amplified Fragment (SLAF) Sequencing and QTL Analysis of Fruit Traits in Cucumber. Frontiers in Plant Science 7: 437-437, 2016

Rapid identification of fruit length loci in cucumber (Cucumis sativus L.) using next-generation sequencing (NGS)-based QTL analysis. Scientific Reports 6: 27496, 2016

Identification of microRNAs associated with the exogenous spermidine-mediated improvement of high-temperature tolerance in cucumber seedlings (Cucumis sativus L.). Bmc Genomics 19(1): 285-285, 2018

Identification and characterization of novel and conserved microRNAs in radish (Raphanus sativus L.) using high-throughput sequencing. Plant Science 201-202: 108-114, 2013

Identification and Characterization of MicroRNAs from Longitudinal Muscle and Respiratory Tree in Sea Cucumber (Apostichopus japonicus) Using High-Throughput Sequencing. Plos One 10(8): E0134899-E0134899, 2016

Identification of Known and Novel microRNAs and Their Targets in Peach (Prunus persica) Fruit by High-Throughput Sequencing. Plos One 11(7): E0159253-E0159253, 2016

Identification of MicroRNAs and Their Targets Associated with Fruit-Bagging and Subsequent Sunlight Re-exposure in the "Granny Smith" Apple Exocarp Using High-Throughput Sequencing. Frontiers in Plant Science 7: 27-27, 2016

Genome-wide identification of bone metastasis-related microRNAs in lung adenocarcinoma by high-throughput sequencing. Plos One 8(4): E61212-E61212, 2013

Genome-wide identification of bone metastasis-related microRNAs in lung adenocarcinoma by high-throughput sequencing. Plos One 8(4): E61212-E61212, 2013

Identification and mapping of molecular markers linked to the tuberculate fruit gene in the cucumber (Cucumis sativus L.). Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 120(3): 645-654, 2010

Identification of MicroRNAs and Their Target Genes Related to the Accumulation of Anthocyanins in iLitchi chinensis by High-Throughput Sequencing and Degradome Analysis. Frontiers in Plant Science 7: 2059-2059, 2017

Identification of Salt Tolerance-related microRNAs and Their Targets in Maize (iZea mays L.) Using High-throughput Sequencing and Degradome Analysis. Frontiers in Plant Science 8: 864-864, 2017

Ontogenic changes in seed weight and carbohydrate composition as related to growth of cucumber (Cucumis sativus L.) fruit. Scientia Horticulturae 63(3/4): 155-165, 1995

Identification and functional characterization of APRR2 controlling green immature fruit color in cucumber (Cucumis sativus L.). Plant Growth Regulation 83(2): 233-243, 2017