+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Identification of functional consequence of a novel selection signature in CYP11b1 gene for milk fat content in Bubalus bubalis



Identification of functional consequence of a novel selection signature in CYP11b1 gene for milk fat content in Bubalus bubalis



Meta Gene 6: 85-90



Genomic selection for traits of economic importance is an emerging approach carrying tremendous potentials. Many of polygenic traits as milk fat, protein and yield have been characterize at genomic level and important selection signatures have been identified. Cytochrome P450 enzymes are potential loci for affecting many of dairy capabilities. Present study was conducted for genomic dissection of CYP11b1 gene in riverine buffaloes and seven genetic variations were identified. Out of these, one novel polymorphism (p.A313T) was found well associated with milk fat %age. AB genotyped buffaloes were found to have higher milk fat %age (8.9%) for this loci. p.A313T was further validated at larger data set by restriction digestion using CviAII enzyme. Functional consequences of this locus were also predicted by studying three dimensional structure of CYP11b1 protein. For this purpose, 3D protein model was predicted by homology modeling, secondary structural attributes were determined, signal peptide was predicted and a transmembrane helix was also identified. One of polymorphism (p.Y205L) was found in the vicinity of functionally significant F-G loop region, which is the part of protein gets attached to the inner mitochondrial membrane. But this variation could not be associated and needs further investigation. p.A30V, a popular selection marker in cattle, was found in buffaloes as well but could not be associated and might need further confirmation on larger data set. Results of this study illustrate the impending potential of this gene in determining dairy capabilities of buffaloes and might have a role in selection of superior dairy buffaloes.

(PDF emailed within 0-6 h: $19.90)

Accession: 058039318

Download citation: RISBibTeXText

PMID: 26629413

DOI: 10.1016/j.mgene.2015.09.002


Related references

A novel selection signature in stearoyl-coenzyme A desaturase (SCD) gene for enhanced milk fat content in Bubalus bubalis. Tropical Animal Health and Production 48(7): 1343-1349, 2017

Identification of polymorphism in fatty acid binding protein 3 (FABP3) gene and its association with milk fat traits in riverine buffalo (Bubalus bubalis). Tropical Animal Health and Production 48(4): 849-853, 2016

Polymorphism in the A2M gene associated with high-quality milk in Murrah buffaloes (Bubalus bubalis). Genetics and Molecular Research 15(2), 2017

Identification of genetic variation in NOD-like receptor 2 gene and influence of polymorphism on gene structure and function in buffalo (Bubalus bubalis). Research in Veterinary Science 115: 43-50, 2017

QUALITY OF REFRIGERATED RAW MILK FROM BUFFALO COWS (Bubalus bubalis bubalis) IN DIFFERENT FARMS AND SEASONS IN BRAZIL. Ciência Animal Brasileira 18(0): e41815-e41815, 2017

Effect of DNA polymorphism in Butyrophilin gene on milk yield in Murrah buffalo (Bubalus bubalis). Indian Journal of Animal Sciences 81(10): 1068-1072, 2011

Association of ss-casein gene polymorphism with milk production traits in buffalo (Bubalus bubalis). Indian Journal of Animal Sciences 77(8): 752-754, 2007

PCR-SSCP analysis of MDGI gene and its association with milk production traits in river buffalo (Bubalus bubalis). Research in Veterinary Science 115: 307-309, 2017

PCR-SSCP analysis of leptin gene and its association with milk production traits in river buffalo (Bubalus bubalis). Tropical Animal Health and Production 44(7): 1587-1592, 2013

Molecular Cloning, Identification, and Expression Patterns of Myostatin Gene in Water Buffalo (Bubalus Bubalis). Animal Biotechnology 29(1): 26-33, 2017

Association analysis of polymorphism in thyroglobulin gene promoter with milk production traits in riverine buffalo (Bubalus bubalis). Meta Gene 5: 157-161, 2015

Short communication: Variable number of tandem repeat polymorphisms in DGAT1 gene of buffaloes (Bubalus bubalis) is associated with milk constituents. Journal of Dairy Science 98(5): 3492-3495, 2016

Identification of a MaeI RFLP in the insulin-like growth factor-1 (IGF1) gene of swamp buffaloes (Bubalus b. bubalis kerebau). Animal Genetics 31(1): 70-71, 2000

Genetic parameters for milk yield of Bubalus bubalis using unadjusted and adjusted milk production for days in milk. Italian Journal of Animal Science 6: 310-313, 2007

Potential functional gene diversity involved in methanogenesis and methanogenic community structure in Indian buffalo (Bubalus bubalis) rumen. Journal of Applied Genetics 56(3): 411-426, 2015