+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Identification of genomic regions involved in tolerance to drought stress and drought stress induced leaf senescence in juvenile barley



Identification of genomic regions involved in tolerance to drought stress and drought stress induced leaf senescence in juvenile barley



Bmc Plant Biology 15: 125



Premature leaf senescence induced by external stress conditions, e.g. drought stress, is a main factor for yield losses in barley. Research in drought stress tolerance has become more important as due to climate change the number of drought periods will increase and tolerance to drought stress has become a goal of high interest in barley breeding. Therefore, the aim is to identify quantitative trait loci (QTL) involved in drought stress induced leaf senescence and drought stress tolerance in early developmental stages of barley (Hordeum vulgare L.) by applying genome wide association studies (GWAS) on a set of 156 winter barley genotypes. After a four weeks stress period (BBCH 33) leaf colour as an indicator of leaf senescence, electron transport rate at photosystem II, content of free proline, content of soluble sugars, osmolality and the aboveground biomass indicative for drought stress response were determined in the control and stress variant in greenhouse pot experiments. Significant phenotypic variation was observed for all traits analysed. Heritabilities ranged between 0.27 for osmolality and 0.61 for leaf colour in stress treatment and significant effects of genotype, treatment and genotype x treatment were estimated for most traits analysed. Based on these phenotypic data and 3,212 polymorphic single nucleotide polymorphisms (SNP) with a minor allele frequency >5% derived from the Illumina 9 k iSelect SNP Chip, 181 QTL were detected for all traits analysed. Major QTLs for drought stress and leaf senescence were located on chromosome 5H and 2H. BlastX search for associated marker sequences revealed that respective SNPs are in some cases located in proteins related to drought stress or leaf senescence, e.g. nucleotide pyrophosphatase (AVP1) or serine/ threonin protein kinase (SAPK9). GWAS resulted in the identification of many QTLs involved in drought stress and leaf senescence of which two major QTLs for drought stress and leaf senescence were located on chromosome 5H and 2H. Results may be the basis to incorporate breeding for tolerance to drought stress or leaf senescence in barley breeding via marker based selection procedures.

(PDF emailed within 0-6 h: $19.90)

Accession: 058039467

Download citation: RISBibTeXText

PMID: 25998066

DOI: 10.1186/s12870-015-0524-3


Related references

Expression profiling of genes involved in drought stress and leaf senescence in juvenile barley. Bmc Plant Biology 16: 3, 2016

Identification of a drought responsive gene encoding a nuclear protein involved in drought and freezing stress tolerance inArabidopsis. Biologia Plantarum 60(1): 105-112, 2016

A Potential Role of Flag Leaf Potassium in Conferring Tolerance to Drought-Induced Leaf Senescence in Barley. Frontiers in Plant Science 7: 206, 2016

Knockdown of WHIRLY1 Affects Drought Stress-Induced Leaf Senescence and Histone Modifications of the Senescence-Associated Gene HvS40. Plants 5(3), 2016

The putative auxin efflux carrier OsPIN3t is involved in the drought stress response and drought tolerance. Plant Journal 72(5): 805-816, 2013

Reduced nitric oxide levels during drought stress promote drought tolerance in barley and is associated with elevated polyamine biosynthesis. Scientific Reports 7, 2017

Identification and functional expression of the pepper pathogen-induced gene, CAPIP2, involved in disease resistance and drought and salt stress tolerance. Plant Molecular Biology 62(1-2): 151-164, 2006

Leaf optical properties during and after drought stress in triticale and maize genotypes differing in drought tolerance. Acta Physiologiae Plantarum 32(3): 433-442, 2010

Evaluation of juvenile drought stress tolerance and genotyping by sequencing with wild barley introgression lines. Molecular Breeding 34(3): 1475-1495, 2014

Analysis of Drought-Induced Proteomic and Metabolomic Changes in Barley (Hordeum vulgare L.) Leaves and Roots Unravels Some Aspects of Biochemical Mechanisms Involved in Drought Tolerance. Frontiers in Plant Science 7: 1108, 2016

Identification of drought tolerance determinants by genetic analysis of root response to drought stress and abscisic Acid. Plant Physiology 142(3): 1065-1074, 2006

The ER luminal binding protein (BiP) mediates an increase in drought tolerance in soybean and delays drought-induced leaf senescence in soybean and tobacco. Journal of Experimental Botany 60(2): 533-546, 2008

Identification of quantitative trait loci associated with drought tolerance traits in rice (Oryza sativa L.) under PEG and field drought stress. Euphytica 214(4): 74, 2018

Differentially expressed genes between drought-tolerant and drought-sensitive barley genotypes in response to drought stress during the reproductive stage. Journal of Experimental Botany 60(12): 3531-3544, 2009

Characterization of Barley Leaf Tolerance to Drought Stress by Chlorophyll Fluorescence and Electron Paramagnetic Resonance Studies. Journal of Agronomy and Crop Science 201(3): 228-240, 2015