+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Identification of human papillomavirus-16 E6 variation in cervical cancer and their impact on T and B cell epitopes



Identification of human papillomavirus-16 E6 variation in cervical cancer and their impact on T and B cell epitopes



Journal of Virological Methods 218: 51-58



The infection with high-risk human papillomavirus (HR-HPV) is the most important risk factor for development of cervical cancer. The intra-type variations of HPV have different biological and pathological consequences with respect to disease progression. In the present study, six major Indian variants were experimentally identified in E6 gene of HPV-16 and showed their impact on immunogenicity by in silico methods. Four different phylogenetic lineages were observed in sequences including European (E) prototype, European variant, Asian and American Asian variant classes and complete absence of African phylogenetic lineages. On the prediction of B- and T-cell epitopes, 18 and 23 potent epitopes for MHC-II alleles, 10 potent MHC-I and 15 B-cell epitopes in each reference and variant sequence were identified. Interestingly, the presence of variation H78Y and L83V result in creation of four new epitopes for the HLA-DQA1*0101/DQB1*0501. Out of 15 B-cell predicted epitopes, three most potent epitopes were identified in both reference and variant sequence. Notably the amino acid stretch from amino acid 16-60 and 76-94 are very important for the immunological properties of E6 protein because these regions contain majority of the predicted epitopes. In future, this could control the cervical cancer by targeting these amino acid stretches for the development of HPV-16 vaccine.

(PDF emailed within 0-6 h: $19.90)

Accession: 058039589

Download citation: RISBibTeXText

PMID: 25800725

DOI: 10.1016/j.jviromet.2015.03.008


Related references

A public t helper epitope of the e7 transforming protein of human papillomavirus 16 provides cognate help for several e7 b cell epitopes from cervical cancer associated human papillomavirus genotypes. Proceedings of the National Academy of Sciences of the United States of America 88(13): 5887-5891, 1991

Human Papillomavirus Genome-Wide Identification of T-Cell Epitopes for Peptide Vaccine Development Against Cervical Cancer: An Integration of Computational Analysis and Experimental Assay. Journal of Computational Biology 22(10): 962-974, 2016

Identification of novel epitopes from human papillomavirus type 18 E7 that can sensitize PBMCs of multiple HLA class I against human cervical cancer. Journal of Translational Medicine 12(): 229-229, 2015

Identification of novel immunogenic human leukocyte antigen-A 2402-binding epitopes of human papillomavirus type 16 E7 for immunotherapy against human cervical cancer. Cancer 118(8): 2173-2183, 2012

Identification of the impact on T- and B- cell epitopes of human papillomavirus type-16 E6 and E7 variant in Southwest China. Immunology Letters 181(): 26-30, 2016

Natural T-helper immunity against human papillomavirus type 16 (HPV16) E7-derived peptide epitopes in patients with HPV16-positive cervical lesions: Identification of 3 human leukocyte antigen class II-restricted epitopes. International Journal of Cancer 91(5): 612-618, 2001

Transcription of human papillomavirus type 16 early genes in a cervical cancer and a cancer-derived cell line and identification of the E7 protein. Proceedings of the National Academy of Sciences of the United States of America 83(13): 4680-4684, 1986

Comparative analysis of cervical cancer in women and in a human papillomavirus-transgenic mouse model: identification of minichromosome maintenance protein 7 as an informative biomarker for human cervical cancer. Cancer Research 63(23): 8173-8180, 2003

Paucity of functional CTL epitopes in the E7 oncoprotein of cervical cancer associated human papillomavirus type 16. Immunology and Cell Biology 81(1): 1-7, 2003

T-helper epitopes identified within the E6 transforming protein of cervical cancer-associated human papillomavirus type 16. Viral Immunology 12(4): 297-312, 2000

T-helper epitopes of the E7 transforming protein of cervical cancer associated human papillomavirus type 18 (HPV18). Virus Research 36(1): 1-13, 1995

Association of serum antibodies against defined epitopes of human papillomavirus L1, E2, and E7 antigens and of HPV DNA with incident cervical cancer. Cancer Detection and Prevention 19(5): 381-393, 1995