+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Identification of immediate early response protein 2 as a regulator of angiogenesis through the modulation of endothelial cell motility and adhesion

Identification of immediate early response protein 2 as a regulator of angiogenesis through the modulation of endothelial cell motility and adhesion

International Journal of Molecular Medicine 36(4): 1104-1110

Human immediate early response 2 (IER2) has been characterized as a putative nuclear protein that functions as a transcription factor or transcriptional co‑activator in the regulation of cellular responses, and may be involved in the regulation of tumor progression and metastasis. Data from our previous gene expression profile of the human microvascular endothelial cells during capillary morphogenesis showed a significant alteration of IER2 expression, suggesting that IER2 may participate in the regulation of the endothelial cell morphogenesis and angiogenesis. The aim of the present study was to investigate the role of IER2 in cell motility, cell‑matrix adhesion and in vitro capillary‑like structures formation of the human umbilical vein endothelium cells (HUVECs). IER2 was constitutively expressed in HUVECs, and lentiviral‑mediated depletion of IER2 significantly reduced the cell motility, cell‑matrix adhesion and capillary‑like structures formation of HUVECs. Results also showed that depletion and overexpression of IER2 altered the actin cytoskeleton rearrangement in HUVECs. Furthermore, results from western blot analysis showed that the activity of the focal adhesion kinase (FAK) can be regulated by IER2. These results indicated that IER2 regulates endothelial cell motility, adhesion on collagen type I matrix and the capillary tube formation, as the result of the regulation of the actin cytoskeleton rearrangement presumably via a FAK‑dependent mechanism.

(PDF emailed within 0-6 h: $19.90)

Accession: 058039621

Download citation: RISBibTeXText

PMID: 26260137

DOI: 10.3892/ijmm.2015.2310

Related references

Identification of protein kinase D2 as a pivotal regulator of endothelial cell proliferation, migration, and angiogenesis. Journal of Biological Chemistry 284(2): 799-806, 2008

Identification of the early response gene EDG-3/MAD-3/I-kappa-B-alpha as a regulator of endothelial cell growth. FASEB Journal 8(7): A1462, 1994

Platelet thrombospondin modulates endothelial cell adhesion, motility, and growth: a potential angiogenesis regulatory factor. Journal of Cell Biology 111(2): 765-772, 1990

Effect of focal adhesion proteins on endothelial cell adhesion, motility and orientation response to cyclic strain. Annals of Biomedical Engineering 38(1): 208-222, 2010

Role of platelet-endothelial cell adhesion molecule and the protein tyrosine phosphatase SHP2 in endothelial cell motility and morphology. FASEB Journal 16(4): A209, March 20, 2002

Chemical structure of flavonols in relation to modulation of angiogenesis and immune-endothelial cell adhesion. Journal of Nutritional Biochemistry 17(3): 165-176, 2005

Platelet endothelial cell adhesion molecule-1 modulates endothelial cell motility through the small G-protein Rho. Faseb Journal 17(11): 1458-1469, 2003

Immediate early response protein 2 regulates hepatocellular carcinoma cell adhesion and motility via integrin β1-mediated signaling pathway. Oncology Reports: -, 2016

Modulation of endothelial cell adhesion by hevin, an acidic protein associated with high endothelial venules. Journal of Biological Chemistry 271(8): 4511-4517, 1996

BMPER is an endothelial cell regulator and controls bone morphogenetic protein-4-dependent angiogenesis. Circulation Research 103(8): 804-812, 2008

Identification of RhoGAP22 as an Akt-dependent regulator of cell motility in response to insulin. Molecular and Cellular Biology 31(23): 4789-4800, 2011

The correlation of epithelial cell adhesion molecule E-cadherin , autocrine motility factor receptor AMFR and cell molecular adhesion regulator CMAR expression with in vitro cell migration in endometrial carcinoma cell lines. Proceedings of the American Association for Cancer Research Annual Meeting 38(0): 289, 1997

Modulation of endothelial cell proliferation, adhesion, and motility by recombinant heparin-binding domain and synthetic peptides from the type I repeats of thrombospondin. Journal of Cellular Biochemistry 53(1): 74-84, 1993

Modulation of expression of endothelial intercellular adhesion molecule-1, platelet-endothelial cell adhesion molecule-1, and vascular cell adhesion molecule-1 in aortic arch lesions of apolipoprotein E-deficient compared with wild-type mice. Arteriosclerosis, Thrombosis, and Vascular Biology 20(10): 2288-2296, 2000