+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Identification of keratan sulfate disaccharide at C-3 position of glucuronate of chondroitin sulfate from Mactra chinensis



Identification of keratan sulfate disaccharide at C-3 position of glucuronate of chondroitin sulfate from Mactra chinensis



Biochemical Journal 473(22): 4145-4158



Glycosaminoglycans (GAGs), including chondroitin sulfate (CS), dermatan sulfate, heparin, heparan sulfate and keratan sulfate (KS) are linear sulfated repeating disaccharide sequences containing hexosamine and uronic acid [or galactose (Gal) in the case of KS]. Among the GAGs, CS shows structural variations, such as sulfation patterns and fucosylation, which are responsible for their physiological functions through CS interaction with CS-binding proteins. Here, we solved the structure of KS-branched CS-E derived from a clam, Mactra chinensis KS disaccharide [d-GlcNAc6S-(1→3)-β-d-Gal-(1→] was attached to the C-3 position of GlcA, and consecutive KS-branched disaccharide sequences were found in a CS chain. KS-branched polysaccharides clearly exhibited resistance to degradation by chondroitinase ABC or ACII (at low concentrations) compared with typical CS structures. Furthermore, KS-branched polysaccharides stimulated neurite outgrowth of hippocampal neurons. These results strongly suggest that M. chinensis is a rich source of KS-branched CS, and it has important biological activities.

(PDF emailed within 0-6 h: $19.90)

Accession: 058039722

Download citation: RISBibTeXText

PMID: 27647934

DOI: 10.1042/BCJ20160655


Related references

Immunolocalization of keratan sulfate, chondroitin-4-sulfate, and chondroitin-6-sulfate in periprosthetic breast capsules exhibiting synovial metaplasia. Plastic and Reconstructive Surgery 98(1): 78-82, 1996

1 dimensional thin layer cellulose chromatographic separation of dermatan sulfate heparin sulfate chondroitin sulfate and keratan sulfate. Taiwan yixuehui zazhi 83(8): 737-742, 1984

Identification of an O-glycosidic mannose-linked sialylated tetrasaccharide and keratan sulfate oligosaccharides in the chondroitin sulfate proteoglycan of brain. Journal of Biological Chemistry 261(18): 8237-8242, 1986

Isolation and characterization of developmentally regulated chondroitin sulfate and chondroitin/keratan sulfate proteoglycans of brain identified with monoclonal antibodies. Journal of Biological Chemistry 266(22): 14785-14801, 1991

Chondroitin sulfate and chondroitin/keratan sulfate proteoglycans of nervous tissue: Developmental changes of neurocan and phosphacan. Journal of Neurochemistry 65(5): 2327-2337, 1995

Biosynthesis of keratan sulfate, chondroitin sulfate and dermatan sulfate proteoglycans. Postepy Biochemii 50(2): 170-181, 2004

Quantitative analysis of glycosaminoglycans, chondroitin/dermatan sulfate, hyaluronic acid, heparan sulfate, and keratan sulfate by liquid chromatography-electrospray ionization-tandem mass spectrometry. Analytical Biochemistry 467: 62-74, 2015

Chemical sulfation of preparations of chondroitin 4 sulfate and 6 sulfate and dermatan sulfate preparation of chondroitin sulfate e like materials from chondroitin 4 sulfate. Carbohydrate Research 158: 183-190, 1986

Novel tetrasaccharides isolated from squid cartilage chondroitin sulfate E contain unusual sulfated disaccharide units GlcA(3-O-sulfate)b1-3GalNAc(6-O-sulfate) or GlcA(3-O-sulfate)b1-3GalNAc(4,6-O-disulfate). The Journal of Biological Chemistry 272: 656-65, 1997

Isolation and characterization of a novel chondroitin sulfate from squid liver integument rich in N-acetylgalactosamine(4,6-disulfate) and glucuronate(3-sulfate) residues. Carbohydrate Research 344(12): 1526-1532, 2009

Detection of keratan sulfate by immunological methods in commercial chondroitin sulfate preparations. Carbohydrate Polymers 99: 547-552, 2014

Immunohistochemical staining for chondroitin sulfate and keratan sulfate an evaluation of two monoclonal antibodies. Histochemistry 95(6): 585-590, 1991

New micromethods for analysis of heterogeneity in chondroitin sulfate and keratan sulfate from cartilage proteoglycans. Glycobiology 3(5): 534, 1993

Developmental distribution of chondroitin sulfate and keratan sulfate in macaque placentas. FASEB Journal 8(4-5): A872, 1994

Keratan sulfate a functional substitute for chondroitin sulfate in o 2 deficient tissues. Pathologie Biologie 37(6): 742-745, 1989