+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Identification of miRNAs Responsive to Botrytis cinerea in Herbaceous Peony (Paeonia lactiflora Pall.) by High-Throughput Sequencing



Identification of miRNAs Responsive to Botrytis cinerea in Herbaceous Peony (Paeonia lactiflora Pall.) by High-Throughput Sequencing



Genes 6(3): 918-934



Herbaceous peony (Paeonia lactiflora Pall.), one of the world's most important ornamental plants, is highly susceptible to Botrytis cinerea, and improving resistance to this pathogenic fungus is a problem yet to be solved. MicroRNAs (miRNAs) play an essential role in resistance to B. cinerea, but until now, no studies have been reported concerning miRNAs induction in P. lactiflora. Here, we constructed and sequenced two small RNA (sRNA) libraries from two B. cinerea-infected P. lactiflora cultivars ("Zifengyu" and "Dafugui") with significantly different levels of resistance to B. cinerea, using the Illumina HiSeq 2000 platform. From the raw reads generated, 4,592,881 and 5,809,796 sRNAs were obtained, and 280 and 306 miRNAs were identified from "Zifengyu" and "Dafugui", respectively. A total of 237 conserved and 7 novel sequences of miRNAs were differentially expressed between the two cultivars, and we predicted and annotated their potential target genes. Subsequently, 7 differentially expressed candidate miRNAs were screened according to their target genes annotated in KEGG pathways, and the expression patterns of miRNAs and corresponding target genes were elucidated. We found that miR5254, miR165a-3p, miR3897-3p and miR6450a might be involved in the P. lactiflora response to B. cinerea infection. These results provide insight into the molecular mechanisms responsible for resistance to B. cinerea in P. lactiflora.

(PDF emailed within 0-6 h: $19.90)

Accession: 058039968

Download citation: RISBibTeXText

PMID: 26393656

DOI: 10.3390/genes6030918


Related references

Digital Gene Expression Analysis to Screen Disease Resistance-Relevant Genes from Leaves of Herbaceous Peony (Paeonia lactiflora Pall.) Infected by Botrytis cinerea. Plos One 10(7): E0133305, 2016

Molecular identification of Botrytis cinerea, Botrytis paeoniae and Botrytis pseudocinerea associated with gray mould disease in peonies (Paeonia lactiflora Pall.) in Southern Chile. Revista Iberoamericana de Micologia 33(1): 43-47, 2016

Combination of Transcriptome sequencing and iTRAQ proteome reveal the molecular mechanisms determining petal shape in Herbaceous Peony (Paeonia lactiflora Pall.). Bioscience Reports 2018, 2018

Transcriptome sequencing of a chimaera reveals coordinated expression of anthocyanin biosynthetic genes mediating yellow formation in herbaceous peony (Paeonia lactiflora Pall.). Bmc Genomics 15: 689, 2015

Micropropagation of herbaceous peony Paeonia lactiflora Pall. Scientia Horticulturae 148(none), 2012

Polyembryony of herbaceous peony seeds (Paeonia lactiflora Pall.). Korean Journal of Breeding 36(5): 316-319, 2004

Age-associated methylation change of CHI promoter in herbaceous peony ( Paeonia lactiflora Pall). Bioscience Reports 38(5), 2018

Nutritional evaluation of herbaceous peony (Paeonia lactiflora Pall.) petals. Emirates Journal of Food and Agriculture 29(7): 518-531, 2017

Cryopreservation of somatic embryos of the herbaceous peony (Paeonia lactiflora Pall.) by air drying. Cryobiology 53(1): 69-74, 2006

Evaluation of the herbaceous peony paeonia lactiflora pall. for commercial pot plant production. Hortscience 23(3 SECT 2): 781, 1988

Cryopreservation of dormant herbaceous peony (Paeonia lactiflora pall.) shoot-tips by desiccation. Cryo Letters 28(3): 207-213, 2007

Cryopreservation of zygotic embryos of herbaceous peony (Paeonia lactiflora Pall.) by encapsulation-dehydration. Korean Journal of Crop Science 49(4): 354-357, 2004

Seasonal patterns of starch and sugar accumulation in herbaceous peony (Paeonia lactiflora Pall.). Journal of Horticultural Science and Biotechnology 82(3): 365-370, 2007

Herbaceous peony (Paeonia lactiflora Pall.) as an alternative source of oleanolic and ursolic acids. International Journal of Molecular Sciences 12(1): 655-667, 2011

Nano-silver modifies the vase life of cut herbaceous peony (Paeonia lactiflora Pall.) flowers. Protoplasma 255(4): 1001-1013, 2018