+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Identification of protein receptors for coronaviruses by mass spectrometry

Identification of protein receptors for coronaviruses by mass spectrometry

Methods in Molecular Biology 1282: 165-182

As obligate intracellular parasites, viruses need to cross the plasma membrane and deliver their genome inside the cell. This step is initiated by the recognition of receptors present on the host cell surface. Receptors can be major determinants of tropism, host range, and pathogenesis. Identifying virus receptors can give clues to these aspects and can lead to the design of intervention strategies. Interfering with receptor recognition is an attractive antiviral therapy, since it occurs before the viral genome has reached the relative safe haven within the cell. This chapter describes the use of an immunoprecipitation approach with Fc-tagged viral spike proteins followed by mass spectrometry to identify and characterize the receptor for the Middle East respiratory syndrome coronavirus. This technique can be adapted to identify other viral receptors.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 058040800

Download citation: RISBibTeXText

PMID: 25720480

Related references

23rd Sanibel Conference on mass spectrometry: from Fragmentation Mechanisms to Sequencing: Tandem Mass Spectrometry Based Peptide and Protein Identification. Journal of the American Society for Mass Spectrometry 23(4): 575-576, 2012

Coxiella burnetii whole cell lysate protein identification by mass spectrometry and tandem mass spectrometry. Annals of the new York Academy of Sciences 1063: 115-122, 2005

Identification of the 'wrong' active pharmaceutical ingredient in a counterfeit Halfan drug product using accurate mass electrospray ionisation mass spectrometry, accurate mass tandem mass spectrometry and liquid chromatography/mass spectrometry. Rapid Communications in Mass Spectrometry 17(3): 215-221, 2003

Tracing of surfactants in the biological wastewater treatment process and the identification of their metabolites by flow injection-mass spectrometry and liquid chromatography-mass spectrometry and -tandem mass spectrometry. Journal of Chromatography A 926(1): 127-150, 10 August, 2001

Improvements in protein identification confidence and proteome coverage for human liver proteome study by coupling a parallel mass spectrometry/mass spectrometry analysis with multi-dimensional chromatography separation. Analytica Chimica Acta 566(2): 147-156, 2006

Identification of two-dimensionally separated human cerebrospinal fluid proteins by N-terminal sequencing, matrix-assisted laser desorption/ionization - mass spectrometry, nanoliquid chromatography-electrospray ionization-time of flight-mass spectrometry, and tandem mass spectrometry. Electrophoresis 21(11): 2266-2283, 2000

Comparison of gas chromatography-pulsed flame photometric detection-mass spectrometry, automated mass spectral deconvolution and identification system and gas chromatography-tandem mass spectrometry as tools for trace level detection and identification. Journal of Chromatography, A 868(2): 229-247, 2000

Influence of the protein staining in the fast ultrasonic sample treatment for protein identification through peptide mass fingerprint and matrix-assisted laser desorption ionization time of flight mass spectrometry. Journal of Proteome Research 7(5): 2097-2106, 2008

Identification of alkyloxypolyethoxycarboxylates in raw and drinking water by mass spectrometry mass spectrometry and mass determination using fast atom bombardment and nonionic surfactants as internal standards. Analytical Chemistry 63(19): 2095-2099, 1991

A mass spectrometry-based proteomic approach for identification of serine/threonine-phosphorylated proteins by enrichment with phospho-specific antibodies: Identification of a novel protein, Frigg, as a protein kinase a substrate. Molecular & Cellular Proteomics 1(7): 517-527, 2002

Mass processing--an improved technique for protein identification with mass spectrometry data. Journal of Biomolecular Techniques 15(4): 230-237, 2004

Peptide mass fingerprinting: protein identification using MALDI-TOF mass spectrometry. Methods in Molecular Biology 310: 227-240, 2005