+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Immunohistochemical evaluation of experimental Vagococcus salmoninarum infection in rainbow trout (Oncorhynchus mykiss, Walbaum 1792)



Immunohistochemical evaluation of experimental Vagococcus salmoninarum infection in rainbow trout (Oncorhynchus mykiss, Walbaum 1792)



Journal of Fish Diseases 39(5): 547-553



The aim of this study was to investigate the pathogenesis and histopathological and immunohistochemical findings in rainbow trout (Oncorhynchus mykiss) following experimental vagococcosis. For this purpose, 60 rainbow trout were used. The experimental study used the pathogen Vagococcus salmoninarum. The fish were intraperitoneally (IP) administered with an inoculate containing 0.1 mL of the bacteria, resulting in a dose of 1.2 × 10(9) cfu mL(-1) per fish. For histopathological observations, tissue samples were taken from fish that died during the experiment and fish that survived until the end of the trial (60th day). All the tissue samples were immunohistochemically stained by the avidin-biotin-peroxidase complex and immunofluorescence methods using polyclonal antibody to detect V. salmoninarum antigens. In immunoperoxidase staining, positive reactions to bacterial antigens were most commonly seen in the kidney, heart and liver. In the immunofluorescence analysis, the distribution of antigens in the tissue and organs was similar to that observed with the immunoperoxidase staining. The results reveal an important correlation between histochemical and immunohistochemical staining in demonstrating the distribution of V. salmoninarum antigens in the affected tissues.

(PDF emailed within 0-6 h: $19.90)

Accession: 058052396

Download citation: RISBibTeXText

PMID: 26095881

DOI: 10.1111/jfd.12386


Related references

Isolation of Vagococcus salmoninarum in rainbow trout, Oncorhynchus mykiss (Walbaum), broodstocks: characterization of the pathogen. Veterinary Research Communications 29(7): 553-562, 2005

Experimental Lactococcus garvieae infection in rainbow trout, Oncorhynchus mykiss, Walbaum 1792: a comparative histopathological and immunohistochemical study. Journal of Fish Diseases 37(5): 481-495, 2014

Examinations of rainbow trout Oncorhynchus mykiss Walbaum, 1792 with using ultrasonography Ultrasonografi ile Gokkusagi Alabaliginin Oncorhynchus mykiss Walbaum, 1792 incelenmesi. Su Urunleri Dergisi, 201-2: 211-216, 2003

Vagococcus salmoninarum, a bacterium of pathological significance in rainbow trout Oncorhynchus mykiss farming. Diseases of Aquatic Organisms 30(3): 199-208, Sept 30, 1997

The relationship and the fecundity between the egg size and the body weight of rainbow trout Oncorhynchus mykiss Walbaum, 1792 and brook trout Salvelinus fontinalis Mitchil, 1814 Gokkusagi Alabaligi Oncorhynchus mykiss Walbaum, 1792 ve Kaynak Alabaligi Salvelinus fontinalis Mitchil, 1814nin Yumurta Capi ile Vucut Buyuklugu Arasindaki Iliski ve Yumurta Verimleri. Su Urunleri Dergisi. 2005( ) Temmuz-Aralik; 223-4: 435-438, 2006

Comparative single-dose pharmacokinetics of orally administered florfenicol in rainbow trout (Oncorhynchus mykiss, Walbaum, 1792) at health and experimental infection with Streptococcus iniae or Lactococcus garvieae. Journal of Veterinary Pharmacology and Therapeutics 2018, 2018

In vitro evaluation of gut contractile response to histamine in rainbow trout (Oncorhynchus mykiss Walbaum, 1792). Research in Veterinary Science 84(1): 126-131, 2007

Statistical evaluation of the effect of heat shock parameters on the effectiveness of triploidy induction in rainbow trout (Oncorhynchus mykiss Walbaum, 1792). Bulletin Vyzkumny Ustav Rybarsky a Hydrobiologicky Vodnany 32(1): 32-38, 1996

Sequential development of the immune response in rainbow trout [Oncorhynchus mykiss (Walbaum, 1792)] to experimental plerocercoid infections of Diphyllobothrium dendriticum (Nitzsch, 1824). Parasitology 104 Pt 1: 169-178, 1992

Sequential development of the immune response in rainbow trout oncorhynchus mykiss walbaum 1792 to experimental plerocercoid infections of diphyllobothrium dendriticum nitzsch 1824. Parasitology 104(1): 169-178, 1992

Acute toxicity of m-cresol on the fry rainbow trout (Oncorhynchus mykiss Walbaum, 1792). Fresenius Environmental Bulletin 14(6): 536-538, 2005

Morphological parameters of the vertebral column in the rainbow trout Oncorhynchus mykiss Teleostei, Salmoniform Descripteurs morphologiques de la zonation de la colonne vertebrale chez la truite arc-en-ciel Oncorhynchus mykiss Walbaum, 1792 Teleostei, Salmoniforme. Annales des Sciences Naturelles Zoologie et Biologie Animale. juillet-septembre; 203: 87-97, 1999

Growth of rainbow trout oncorhynchus mykiss walbaum 1792 related to egg size and temperature. Dana 9: 31-38, 1991

Molecular characterization of rainbow trout,Oncorhynchus mykiss(Walbaum, 1792) stocks in India. Journal of Genetics 94(2 Supplement): 13-18, 2015

Evaluation of Bacillus subtilis Effect as Probiotic on Hematological Parameters of Rainbow Trout, Oncorhynchus mykiss Walbaum Following Experimental Infection with Streptococcus iniae. 2012