+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Improved in vitro evaluation of novel antimicrobials: potential synergy between human plasma and antibacterial peptidomimetics, AMPs and antibiotics against human pathogenic bacteria

Improved in vitro evaluation of novel antimicrobials: potential synergy between human plasma and antibacterial peptidomimetics, AMPs and antibiotics against human pathogenic bacteria

Research in Microbiology 167(2): 72-82

Stable peptidomimetics mimicking natural antimicrobial peptides (AMPs) have emerged as a promising class of potential novel antibiotics. In the present study, we aimed at determining whether the antibacterial activity of two α-peptide/β-peptoid peptidomimetics against a range of bacterial pathogens was affected by conditions mimicking in vivo settings. Their activity was enhanced to an unexpected degree in the presence of human blood plasma for thirteen pathogenic Gram-positive and Gram-negative bacteria. MIC values typically decreased 2- to 16-fold in the presence of a human plasma concentration that alone did not damage the cell membrane. Hence, MIC and MBC data collected in these settings appear to represent a more appropriate basis for in vivo experiments preceding clinical trials. In fact, concentrations of peptidomimetics and peptide antibiotics (e.g. polymyxin B) required for in vivo treatments might be lower than traditionally deduced from MICs determined in laboratory media. Thus, antibiotics previously considered too toxic could be developed into usable last-resort drugs, due to ensuing lowered risk of side effects. In contrast, the activity of the compounds was significantly decreased in heat-inactivated plasma. We hypothesize that synergistic interactions with complement proteins and/or clotting factors most likely are involved.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 058072041

Download citation: RISBibTeXText

PMID: 26499211

DOI: 10.1016/j.resmic.2015.10.002

Related references

In vitro antibacterial potential of some Vitex species against human pathogenic bacteria. Asian Pacific Journal of Tropical Medicine 4(8): 645-648, 2011

In vitro antibacterial potential of alkaloids of Samanea saman (Jacq.) Merr. against Xanthomonas and human pathogenic bacteria. 2008

Antibacterial potential of phytochemicals alone or in combination with antimicrobials against fish pathogenic bacteria. Journal of Applied Microbiology 125(3): 655-665, 2018

Antibacterial activity of some important standard antibiotics against human and plant pathogenic bacteria. Current Agriculture 28(1/2): 75-78, 2004

Evaluation of In-vitro Antimicrobial Potential of Daphne retusa Hemsl. Against Human Pathogenic Bacteria and Fungi. Current Topics in Medicinal Chemistry 18(9): 779-786, 2018

Antibacterial potential of some indigenous plants against human pathogenic bacteria. Environment and Ecology 24S(Special 2): 403-405, 2006

Antibacterial potential of Antarctic lichens against human pathogenic Gram-positive bacteria. PhytoTherapy Research 22(9): 1269-1271, 2008

In vitro susceptibility of new generation of antibacterial antibiotics against pathogenic bacteria. Hindustan Antibiotics Bulletin 41(1-4): 41-44, 2002

Evaluation of biological value and appraisal of polyphenols and glucosinolates from organic baby-leaf salads as antioxidants and antimicrobials against important human pathogenic bacteria. Molecules 18(4): 4651-4668, 2013

Augmentation of the antibacterial activities of Pt5-derived antimicrobial peptides (AMPs) by amino acid substitutions: Design of novel AMPs against MDR bacteria. Fish and Shellfish Immunology 77: 100-111, 2018

Antibacterial and Biofilm Modulating Potential of Ferulic Acid-Grafted Chitosan against Human Pathogenic Bacteria. International Journal of Molecular Sciences 19(8):, 2018

Antibacterial potential of Elettaria cardamomum (Chotti elaichi) against human pathogenic bacteria, Staphylococcus aureus. Journal of Eco Physiology 3(3/4): 135-137, 2000

Antibacterial evaluation and phytochemical analysis of Betula utilis D Don against some human pathogenic bacteria. World Journal Of Agricultural Sciences: 5, 661-664, 2008

Evaluation of antibacterial activity of some traditionally used medicinal plants against human pathogenic bacteria. Biomed Research International 2015: 265425, 2015

In-vitro screening of Malaysian honey from different floral sources for antibacterial activity on human pathogenic bacteria. African Journal of Traditional Complementary and Alternative Medicines 11(2): 315-318, 2014