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In vitro and in vivo methodologies for studying the Sigma 54-dependent transcription



In vitro and in vivo methodologies for studying the Sigma 54-dependent transcription



Methods in Molecular Biology 1276: 53-79



Here we describe approaches and methods to assaying in vitro the major variant bacterial sigma factor, Sigma 54 (σ(54)), in a purified system. We include the complete transcription system, binding interactions between σ54 and its activators, as well as the self-assembly and the critical ATPase activity of the cognate activators which serve to remodel the closed promoter complexes. We also present in vivo methodologies that are used to study the impact of physiological processes, metabolic states, global signalling networks, and cellular architecture on the control of σ(54)-dependent gene expression.

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Accession: 058085426

Download citation: RISBibTeXText

PMID: 25665558

DOI: 10.1007/978-1-4939-2392-2_4



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