+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

In vitro release and In vivo biocompatibility studies of biomimetic multilayered alginate-chitosan/β-TCP scaffold for osteochondral tissue

In vitro release and In vivo biocompatibility studies of biomimetic multilayered alginate-chitosan/β-TCP scaffold for osteochondral tissue

Journal of Biomaterials Science. Polymer Edition 27(5): 431-440

Biomimetic three-layered monolithic scaffold (TLS) intended for the treatment of osteocondral defects was prepared by using alginate, chitosan and β-tricalcium phosphate (β-TCP) to study drug release behavior of the alternative drug delivery system and to investigate the therapeutic efficacy of the scaffold. Dexamethasone sodium phosphate (Dex) as a model drug was incorporated into the scaffold by solvent sorption method and in vitro release studies were conducted. In addition, the scaffold was implanted into the defects formed in the trochlea of Sprague-Dawley rats to assess the healing potential of the TLS on the osteochondral defect against reference Maioregen® comparatively. The release studies showed that after an initial burst at 3rd h, dexamethasone is released slowly during a 72-h period. In vivo studies indicated that the TLS has good tissue biocompatibility and biodegradation rate and showed better results during osteochondral healing process compared to the reference. All results demonstrated that the alginate-chitosan/β-TCP scaffold could be evaluated as a good candidate for osteochondral tissue applications.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 058086949

Download citation: RISBibTeXText

PMID: 26764607

DOI: 10.1080/09205063.2016.1140501

Related references

Biocompatibility of biomimetic multilayered alginate-chitosan/β-TCP scaffold for osteochondral tissue. International Journal of Biological Macromolecules 79: 363-369, 2015

Preparation and biocompatibility of a novel biomimetic osteochondral scaffold: collagen-chitosan/nano-hydroxyapatite-collagen-polylactic acid. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 26(8): 1001-1006, 2012

In vitro generation of a multilayered osteochondral construct with an osteochondral interface using rabbit bone marrow stromal cells and a silk peptide-based scaffold. Journal of Tissue Engineering and Regenerative Medicine 10(4): 284-293, 2016

Evaluation of a biomimetic poly(ε-caprolactone)/β-tricalcium phosphate multispiral scaffold for bone tissue engineering: in vitro and in vivo studies. Biointerphases 9(2): 029011, 2014

Morphogenetically active scaffold for osteochondral repair (polyphosphate/alginate/N,O-carboxymethyl chitosan). European Cells and Materials 31: 174-190, 2016

Biocompatibility study of a silk fibroin-chitosan scaffold with adipose tissue-derived stem cells in vitro. Experimental and Therapeutic Medicine 6(2): 513-518, 2013

A novel porous collagen scaffold around an implantable biosensor for improving biocompatibility. I. In vitro/in vivo stability of the scaffold and in vitro sensitivity of the glucose sensor with scaffold. Journal of Biomedical Materials Research. Part a 87(1): 136-146, 2008

Carbon nanofiber amalgamated 3D poly-ε-caprolactone scaffold functionalized porous-nanoarchitectures for human meniscal tissue engineering: In vitro and in vivo biocompatibility studies. Nanomedicine 14(7): 2247-2258, 2018

Fabrication and in vivo osteogenesis of biomimetic poly(propylene carbonate) scaffold with nanofibrous chitosan network in macropores for bone tissue engineering. Journal of Materials Science. Materials in Medicine 23(2): 517-525, 2012

Construction of a fluorescent nanostructured chitosan-hydroxyapatite scaffold by nanocrystallon induced biomimetic mineralization and its cell biocompatibility. Acs Applied Materials and Interfaces 3(5): 1692-1701, 2011

In vitro biocompatibility and release of curcumin from curcumin microcomplex-loaded chitosan scaffold. Journal of Microencapsulation 32(4): 364-371, 2015

A new bi-layered scaffold for osteochondral tissue regeneration: In vitro and in vivo preclinical investigations. Materials Science and Engineering. C Materials for Biological Applications 70(Pt 1): 101-111, 2017

Novel hydroxyapatite/chitosan bilayered scaffold for osteochondral tissue-engineering applications: Scaffold design and its performance when seeded with goat bone marrow stromal cells. Biomaterials 27(36): 6123-6137, 2006

Evaluation of adenoviral vascular endothelial growth factor-activated chitosan/hydroxyapatite scaffold for engineering vascularized bone tissue using human osteoblasts: In vitro and in vivo studies. Journal of Biomaterials Applications 29(5): 748-760, 2014

Novel chitosan hydrogel formed by ethylene glycol chitosan, 1,6-diisocyanatohexan and polyethylene glycol-400 for tissue engineering scaffold: in vitro and in vivo evaluation. Journal of Materials Science. Materials in Medicine 25(8): 1903-1913, 2014