+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

In vivo genotoxicity assessment of acrylamide and glycidyl methacrylate



In vivo genotoxicity assessment of acrylamide and glycidyl methacrylate



Food and Chemical Toxicology 87: 120-127



Acrylamide (ACR) and glycidyl methacrylate (GMA) are structurally related compounds used for making polymers with various properties. Both chemicals can be present in food either as a byproduct of processing or a constituent of packaging. We performed a comprehensive evaluation of ACR and GMA genotoxicity in Fisher 344 rats using repeated gavage administrations. Clastogenicity was measured by scoring micronucleated (MN) erythrocytes from peripheral blood, DNA damage in liver, bone marrow and kidneys was measured using the Comet assay, and gene mutation was measured using the red blood cell (RBC) and reticulocyte Pig-a assay. A limited histopathology evaluation was performed in order to determine levels of cytotoxicity. Doses of up to 20 mg/kg/day of ACR and up to 250 mg/kg/day of GMA were used. ACR treatment resulted in DNA damage in the liver, but not in the bone marrow. While ACR was not a clastogen, it was a weak (equivocal) mutagen in the cells of bone marrow. GMA caused DNA damage in the cells of bone marrow, liver and kidney, and induced MN reticulocytes and Pig-a mutant RBCs in a dose-dependent manner. Collectively, our data suggest that both compounds are in vivo genotoxins, but the genotoxicity of ACR is tissue specific.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 058088027

Download citation: RISBibTeXText

PMID: 26686995

DOI: 10.1016/j.fct.2015.12.006


Related references

Genotoxicity of glycidyl methacrylate. Zhongguo Yaolixue Yu Dulixue Zazhi 4(3): 225-228, 1990

Cytotoxicity and genotoxicity of glycidyl methacrylate. Chemico-Biological Interactions 180(1): 69-78, 2009

Studies of the genotoxicity of glycidyl methacrylate (GMA). Biomedical and Environmental Sciences 3(3): 281-289, 1990

Method of screening substances with a glycidyl methacrylate covered styrene-glycidyl methacrylate polymer. Official Gazette of the United States Patent and Trademark Office Patents 1280(2), 2004

Synthesis of a novel superabsorbent hydrogel by copolymerization of acrylamide and cashew gum modified with glycidyl methacrylate. Carbohydrate Polymers 61(4): 464-471, 2005

Adsorption of Ag(I) on glycidyl methacrylate/N,N-methylene bis-acrylamide chelating resins with embedded iron oxide. Separation and Purification Technology 48(3): 281-287, 2006

Dose-response modeling of in vivo genotoxicity data for use in risk assessment: some approaches illustrated by an analysis of acrylamide. Regulatory Toxicology and Pharmacology 41(1): 6-27, 2005

Formation of glycidyl methacrylate-DNA adducts in vivo. Biomedical and Environmental Sciences 12(2): 95-102, 1999

A murine glaucoma model induced by rapid in vivo photopolymerization of hyaluronic acid glycidyl methacrylate. Plos one 13(6): E0196529, 2018

Preparation and Properties of Cryogel Based on Poly(2-hydroxyethyl methacrylate- co-glycidyl methacrylate). Langmuir 35(9): 3284-3294, 2019

Immobilization of enzymes on 2-hydroxyethyl methacrylate and glycidyl methacrylate copolymer brushes. Chemistry An Asian Journal 9(8): 2132-2139, 2014

Studies on novel radiopaque methyl methacrylate: glycidyl methacrylate based polymer for biomedical applications. Journal of Materials Science. Materials in Medicine 20(Suppl. 1): S243, 2009

Immobilization of amylo glucosidase ec 3.2.1.3 on poly glycidyl methacrylate co ethylene di methacrylate carrier and its derivatives. Biotechnology and Bioengineering 20(9): 1319-1328, 1978

Immobilization of horse liver alcohol dehydrogenase ec 1.1.1.1 on co polymers of glycidyl methacrylate and ethylene di methacrylate. Biotechnology and Bioengineering 24(4): 837-846, 1982

Functionalized cellulose with hydroxyethyl methacrylate and glycidyl methacrylate for metal ions and dye adsorption applications. International Journal of Biological Macromolecules 134: 704-721, 2019