+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Incorporation of spike and membrane glycoproteins into coronavirus virions



Incorporation of spike and membrane glycoproteins into coronavirus virions



Viruses 7(4): 1700-1725



The envelopes of coronaviruses (CoVs) contain primarily three proteins; the two major glycoproteins spike (S) and membrane (M), and envelope (E), a non-glycosylated protein. Unlike other enveloped viruses, CoVs bud and assemble at the endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC). For efficient virion assembly, these proteins must be targeted to the budding site and to interact with each other or the ribonucleoprotein. Thus, the efficient incorporation of viral envelope proteins into CoV virions depends on protein trafficking and protein-protein interactions near the ERGIC. The goal of this review is to summarize recent findings on the mechanism of incorporation of the M and S glycoproteins into the CoV virion, focusing on protein trafficking and protein-protein interactions.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 058094519

Download citation: RISBibTeXText

PMID: 25855243

DOI: 10.3390/v7041700


Related references

A single amino acid mutation in the spike protein of coronavirus infectious bronchitis virus hampers its maturation and incorporation into virions at the nonpermissive temperature. Virology 326(2): 288-298, 2004

Deficient incorporation of spike protein into virions contributes to the lack of infectivity following establishment of a persistent, non-productive infection in oligodendroglial cell culture by murine coronavirus. Virology 409(1): 121-131, 2011

A conditional-lethal murine coronavirus mutant that fails to incorporate the spike glycoprotein into assembled virions. Virus Research. 39(2-3): 261-276, 1995

The spike protein of transmissible gastroenteritis coronavirus controls the tropism of pseudorecombinant virions engineered using synthetic minigenomes. Advances in Experimental Medicine and Biology 440: 207-214, 1998

Sialic acid binding activity of transmissible gastroenteritis coronavirus affects sedimentation behavior of virions and solubilized glycoproteins. Journal of Virology 75(2): 844-849, 2001

Reasoning of spike glycoproteins being more vulnerable to mutations among 158 coronavirus proteins from different species. Journal of Molecular Modeling 11(1): 8, 2005

LSECtin interacts with filovirus glycoproteins and the spike protein of SARS coronavirus. Virology 340(2): 224-236, 2005

Primary structures of hemagglutinin-esterase and spike glycoproteins of murine coronavirus DVIM. Virus Genes 17(2): 123-128, 1998

Role of HIV-1 matrix protein in selectively incorporation of viral Env and cellular glycoproteins into mature virions. ELEVENTH INTERNATIONAL CONFERENCE ON AIDS [Author] Eleventh International Conference on AIDS, Vol Two One world: One hope : 217, 1996

Electrophoretic pattern of plasma membrane proteins and glycoproteins of chicken liver and hepatoma mc 29 carbon 14 glucosamine and carbon 14 fucose incorporation in membrane glycoproteins. Obshta i Sravnitelna Patologiya 18: 33-42, 1985

Genetic analysis of determinants for spike glycoprotein assembly into murine coronavirus virions: distinct roles for charge-rich and cysteine-rich regions of the endodomain. Journal of Virology 78(18): 9904-9917, 2004

Studies on glycoproteins. V. Incorporation of glucosamine into membrane glycoproteins of phytohemagglutinin-stimulated lymphocytes. Journal of Biological Chemistry 245(21): 5827-5832, 1970

Transmissible gastroenteritis coronavirus genome packaging signal is located at the 5' end of the genome and promotes viral RNA incorporation into virions in a replication-independent process. Journal of Virology 87(21): 11579-11590, 2013

SARS coronavirus spike polypeptide DNA vaccine priming with recombinant spike polypeptide from Escherichia coli as booster induces high titer of neutralizing antibody against SARS coronavirus. Vaccine 23(42): 4959-4968, 2005