+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Interaction of nitric oxide and reactive oxygen species and associated regulation of root growth in wheat seedlings under zinc stress

Interaction of nitric oxide and reactive oxygen species and associated regulation of root growth in wheat seedlings under zinc stress

Ecotoxicology and Environmental Safety 113: 95-102

The inhibition of root growth was investigated in wheat seedlings exposed to 3mM zinc (Zn). Zn treatment with or without 250 µM 2-phenyl-4,4,5,5,-tetrame-thylimidazoline-3-oxide-1-oxyl (PTIO) or 10 µM diphenylene iodonium (DPI) significantly inhibited growth, increased malondialdehyde content and lowered cell viability in roots. The most prominent changes of these three parameters at Zn+DPI treatment could be partly blocked by high PTIO concentration (1mM). The production of nitric oxide (NO) and hydrogen peroxide (H2O2) influenced each other under different treatments, with the highest NO level and the highest H2O2 accumulation in Zn+DPI-treated roots. Compared with Zn-stressed roots, catalase, soluble peroxidase (POD), ascorbate peroxidase and superoxide dismutase decreased in Zn+DPI-treated roots, suggesting that ROS generation from plasma membrane (PM) NADPH oxidase was associated with the regulation of antioxidant enzyme activities. Additionally, Zn-treated roots exhibited significant decreases in cell wall-bound POD, diamine oxidase and polyamine oxidase activities. Our results suggested that Zn-induced effects on root growth resulted from NO interaction with H2O2 and that Zn+DPI-induced strongest inhibition could be explained by the highest increase in the endogenous NO content and the reduction of extracellular ROS production.

(PDF emailed within 0-6 h: $19.90)

Accession: 058135111

Download citation: RISBibTeXText

PMID: 25485957

DOI: 10.1016/j.ecoenv.2014.11.030

Related references

Interaction between reactive oxygen species and nitric oxide in drought-induced abscisic acid synthesis in root tips of wheat seedlings. Australian Journal of Plant Physiology 28(10): 1055-1061, 2001

The roles of ABA, reactive oxygen species and nitric oxide in root growth during osmotic stress in wheat: comparison of a tolerant and a sensitive variety. Acta Biologica Hungarica 61 Suppl: 189-196, 2011

Evidence for the involvement of nitric oxide and reactive oxygen species in osmotic stress tolerance of wheat seedlings: inverse correlation between leaf abscisic acid accumulation and leaf water loss. Plant Growth Regulation 42(1): 61-68, 2004

Effects of nitric oxide on root growth and absorption in wheat seedlings in response to water stress. 2008

Effects of nitric oxide on the germination of wheat seeds and its reactive oxygen species metabolisms under osmotic stress. Acta Botanica Sinica 45(8): 901-905, 2003

The important roles of reactive oxygen species in the relationship between ethylene and polyamines in leaves of spring wheat seedlings under root osmotic stress. Plant Science 166(2): 303-315, 2004

Reactive oxygen species and nitric oxide are involved in polyamine-induced growth inhibition in wheat plants. Protoplasma, 2018

Participation of Nitric Oxide in 24-Epibrassinolide-Induced Heat Resistance of Wheat Coleoptiles: Functional Interactions of Nitric Oxide with Reactive Oxygen Species and Ca Ions. Russian Journal of Plant Physiology 65(2): 177-185, 2018

Both the stimulation and inhibition of root hair growth induced by extracellular nucleotides in Arabidopsis are mediated by nitric oxide and reactive oxygen species. Plant Molecular Biology 74(4-5): 423-435, 2010

Ethylene and reactive oxygen species are involved in root aerenchyma formation and adaptation of wheat seedlings to oxygen-deficient conditions. Journal of Experimental Botany 65(1): 261-273, 2014

Interaction of nitric oxide and reactive oxygen species on rat diaphragm contractility. Acta Physiologica Scandinavica 169(3): 229-236, 2000

Interaction between nitric oxide, reactive oxygen intermediates, and peroxynitrite in the regulation of 5-lipoxygenase metabolism. Biochimica et Biophysica Acta 1584(2-3): 81-90, 10 October, 2002

Interplay of reactive oxygen species and nitric oxide: nitric oxide coordinates reactive oxygen species homeostasis. Plant Physiology 167(4): 1209-1210, 2016

The relationship between reactive oxygen species and nitric oxide in ultraviolet-B-induced ethylene production in leaves of maize seedlings. Environmental and experimental botany 57(1-2): 51-61, 2006

Regulation of cytochrome bd expression in the obligate aerobe Azotobacter vinelandii by CydR (Fnr). Sensitivity to oxygen, reactive oxygen species, and nitric oxide. Journal of Biological Chemistry 275(7): 4679-4686, 2000