+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Interleukin-15-transferred cytokine-induced killer cells elevated anti-tumor activity in a gastric tumor-bearing nude mice model

Interleukin-15-transferred cytokine-induced killer cells elevated anti-tumor activity in a gastric tumor-bearing nude mice model

Cell Biology International 40(2): 204-213

Gastric cancer is the second leading cause of cancer-related mortality worldwide. Adoptive cell therapy (ACT) for gastric cancer is a novel therapy modality. However, the therapeutic effectiveness in vivo is still limited. The objective of this study was to assess the value of interleukin-15 (IL-15)-transferred cytokine-induced killer (CIK) cells in ACT for gastric cancer. IL-15-IRES-TK retroviral vector was constructed and transferred into the CIK cells. A gastric tumor-bearing nude mice model was constructed by subcutaneously injecting gastric cancer cells, BGC-823. Gastric tumor-bearing nude mice were randomly divided into three groups (five mice each group) and injected with physiological saline, CIK cells, and IL-15-IRES-TK-transfected CIK cells for 2 weeks, respectively. IL-15-IRES-TK-transferred CIK cells were prepared successfully and flow cytometry (FCM) analysis indicated that the transfection rate reached 85.7% after 5 days culture. In vivo experiment, we found that CIK cells retarded tumor growth by reducing tumor volume and tumor weight, as well as increasing tumor inhibition rate. Furthermore, IL-15-IRES-TK-transferred CIK cells showed a much stronger inhibition on tumor growth than CIK cells alone. Tumor morphology observation and growth indexes also showed that IL-15-transfected CIK cells had stronger cytotoxicity to tumor tissue than CIK cells. IL-15-IRES-TK transfection could elevate the effects of CIK cells to gastric carcinoma. The engineered CIK cells carrying IL-15-IRES-TK may be used in the ACT for gastric carcinoma, but prudent clinical trial is still indispensable.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 058138738

Download citation: RISBibTeXText

PMID: 26503216

DOI: 10.1002/cbin.10553

Related references

Evaluation of in vivo labeled cytotoxic T lymphocytes and cytokine-induced killer cells migration and distribution of subcutaneous gastric tumor model in nude mice. Zhonghua Yi Xue Za Zhi 90(6): 403-406, 2010

Influence of different ex vivo cell culture methods on the proliferation and anti-tumor activity of cytokine-induced killer cells from gastric cancer patients. Oncotargets and Therapy 11: 2657-2672, 2018

Inhibition of human pancreatic tumor growth by cytokine-induced killer cells in nude mouse xenograft model. Immune Network 12(6): 247-252, 2012

Characteristics of tumor infiltration by adoptively transferred and endogenous natural-killer cells in a syngeneic rat model: Implications for the mechanism behind anti-tumor responses. International Journal of Cancer 78(6): 783-789, 1998

Combination treatment with irritant and recombinant interleukin 2 in the peritoneal cavity for evoking effective anti-tumor activity: generation of lymphokine-activated killer cells and tumor-specific killer cells. Japanese Journal of Cancer Research 81(4): 416-424, 1990

Immunomagnetic selection or irradiation eliminates alloreactive cells but also reduces anti-tumor potential of cytokine-induced killer cells: implications for unmanipulated cytokine-induced killer cell infusion. CytoTherapy 16(6): 835-844, 2014

Effect of anti-asthma Chinese medicine Chuankezhi on the anti-tumor activity of cytokine-induced killer cells. Chinese Journal of Cancer 32(10): 553-560, 2013

The proliferation, phenotype change and anti-tumor activity of cytokine induced killer cells. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 21(5): 583-586, 2005

Cisplatin pretreatment enhances anti-tumor activity of cytokine-induced killer cells. World Journal of Gastroenterology 17(25): 3002-3011, 2011

The enhancement of cyclophosphamides anti-tumor activity by interleukin-1-alpha in RIF-1 tumor bearing C3H/HeJ mice. Proceedings of the American Association for Cancer Research Annual Meeting 35: 297, 1994

Enhancement of anti-tumor immunity by lipoteichoic acid-related molecule isolated from OK-432, a streptococcal agent, in athymic nude mice bearing human salivary adenocarcinoma: role of natural killer cells. Anticancer Research 22(6a): 3229-3239, 2003

Traffic and tumor localization of adoptively transferred IL2-activated natural killer cells in B16 melanoma bearing mice. Proceedings of the American Association for Cancer Research Annual Meeting 42: 329, 2001

Anti-tumor activity of ex vivo expanded cytokine-induced killer cells against human hepatocellular carcinoma. International Immunopharmacology 7(13): 1793-1801, 2007

Anti tumor activity of yeast cell wall against syngeneic tumor and its effect on mitogenic response of spleen cells from tumor bearing mice. Yakugaku Zasshi 100(6): 619-624, 1980

Experimental studies and clinical investigations on natural killer (NK) activity--NK activity in tumor bearing nude mice and in patients with cancer of digestive organs. Igaku Kenkyu. Acta Medica 54(3): 301-316, 1984