+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Intronic promoter-mediated feedback loop regulates bean PvSR2 gene expression

Intronic promoter-mediated feedback loop regulates bean PvSR2 gene expression

Biochemical and Biophysical Research Communications 463(4): 1097-1101

Intronic promoter usage is a widespread regulatory mechanism for increasing the diversity of gene expression regulation. However, its trans-acting mechanisms that modulate host gene expression are poorly characterized. We previously showed that bean (Phaseolus vulgaris) stress-related gene 2 (PvSR2) produces a metal-responsive element (MRE)-binding transcription factor (PvMTF-1) by using an intronic promoter. Interestingly, a functional MRE resides within the upstream PvSR2 promoter, implying that PvMTF-1 is likely to regulate its host gene expression via this MRE. Through DNA-protein interaction studies combined with an analysis of expression of PvMTF-1 in bean leaves, we now report that PvMTF-1 binds to this MRE and thereby up-regulates PvSR2 expression, thus constituting a positive regulatory feedback loop. Our findings provide evidence for a trans-acting mechanism of an intronic promoter in host gene expression, hence, adding a new layer of complexity in intronic promoter-mediated gene regulation. This study may also contribute to the understanding of the intron-mediated regulation of gene expression in trans.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 058154893

Download citation: RISBibTeXText

PMID: 26079876

DOI: 10.1016/j.bbrc.2015.06.064

Related references

The bean PvSR2 gene produces two transcripts by alternative promoter usage. Biochemical and Biophysical Research Communications 356(1): 273-278, 2007

A complex intronic enhancer regulates expression of the CFTR gene by direct interaction with the promoter. Journal of Cellular and Molecular Medicine 13(4): 680-692, 2009

Cloning and expression analysis of the heavy-metal responsive gene PvSR2 from bean. Plant Science 161(4): 783-790, 2001

A negative feedback loop at the nuclear periphery regulates GAL gene expression. Molecular Biology of the Cell 23(7): 1367-1375, 2012

The exosome regulates circadian gene expression in a posttranscriptional negative feedback loop. Cell 138(6): 1236-1246, 2009

PIL1 participates in a negative feedback loop that regulates its own gene expression in response to shade. Molecular Plant 7(10): 1582-1585, 2014

Twist Regulates Cytokine Gene Expression through a Negative Feedback Loop that Represses NF-kB Activity. Cell 112(2): 9-80, 2003

Twist regulates cytokine gene expression through a negative feedback loop that represses NF-kappaB activity. Cell 112(2): 169-180, 2003

A novel pineal-specific product of the oligopeptide transporter PepT1 gene: circadian expression mediated by cAMP activation of an intronic promoter. Journal of Biological Chemistry 280(17): 16851-16860, 2005

A double feedback loop mediated by microRNA-23a/27a/24-2 regulates M1 versus M2 macrophage polarization and thus regulates cancer progression. Oncotarget 7(12): 13502-13519, 2016

A feedback loop regulates the switch from one sigma factor to the next in the cascade controlling Bacillus subtilis mother cell gene expression. Journal of Bacteriology 179(19): 6138-6144, 1997

A feedback loop consisting of microRNA 23a/27a and the β-like globin suppressors KLF3 and SP1 regulates globin gene expression. Molecular and Cellular Biology 33(20): 3994-4007, 2013

Zn stress-induced inhibition of bean PvSR2-GUS fusion gene splicing is gene-specific in transgenic tobacco. Journal of Plant Physiology 166(11): 1223-1227, 2009

Intronic miR-301 feedback regulates its host gene, ska2, in A549 cells by targeting MEOX2 to affect ERK/CREB pathways. Biochemical and Biophysical Research Communications 396(4): 978-982, 2010

Sp1 regulates chromatin looping between an intronic enhancer and distal promoter of the human heme oxygenase-1 gene in renal cells. Journal of Biological Chemistry 285(22): 16476-16486, 2010