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Loop-Mediated Isothermal Amplification (LAMP) for Detection of Phytoplasmas in the Field

Loop-Mediated Isothermal Amplification (LAMP) for Detection of Phytoplasmas in the Field

Methods in Molecular Biology 1302: 99-111

Loop-mediated isothermal amplification (LAMP) is a nucleic acid-based detection method with many applications. This chapter details its use for detection of phytoplasma diseases, combining a rapid 2-min DNA extraction method with real-time LAMP product detection such that the entire procedure can be undertaken and completed in the field within 40 min. Furthermore, the assays include an anneal curve validation step to guard against false positives and an assay for host plant DNA to guard against false negatives.

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Accession: 058238599

Download citation: RISBibTeXText

PMID: 25981249

DOI: 10.1007/978-1-4939-2620-6_8

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