+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Lycopene protects against atrazine-induced hepatic ionic homeostasis disturbance by modulating ion-transporting ATPases



Lycopene protects against atrazine-induced hepatic ionic homeostasis disturbance by modulating ion-transporting ATPases



Journal of Nutritional Biochemistry 27: 249-256



The aim of this study was to evaluate the possible chemoprotective role of lycopene (LYC) against atrazine (ATR)-induced ionic disorder and hepatotoxicity in mice. Male kunming mice were treated with LYC (5mg/kg) and/or ATR (50mg/kg or 200mg/kg) by lavage administration for 21days. Ionic disorder was assessed by determining the Na(+), K(+) and Ca(2+) content and the alteration in ATP enzymes (ATPases) including Na(+)-K(+)-ATPase, Ca(2+)-ATPase, Mg(2+)-ATPase and Ca(2+)-Mg(2+)-ATPase and the mRNA levels of ATPase's subunits in liver. ATR caused the increases of alanine aminotransferase and aspartate aminotransferase activities and histological changes. LYC pretreatment significantly protected liver against ATR-caused alternation. The significant effect of ATR and LYC on the K(+) and Mg(2+) content in liver was not observed, but ATR increased hepatic Na(+)-K(+)-ATPase activity and decreased Mg(2+)-ATPase and Ca(2+)-Mg(2+)-ATPase activity. The mRNA expressions of Na(+)-K(+)-ATPase subunits were regulated significantly by ATR. A significant increase of Ca(2+) content and seven down-regulated mRNA expressions of Ca(2+)-ATPase subunits and a decrease of Ca(2+)-ATPase activity were observed in the ATR-treated mice. Notably, LYC modulated these ATR-induced alterations of ATPase activity and mRNA expression of their subunits. These results suggest that ATR presents hepatotoxicity via regulating hepatic ATPase's activities and their subunit transcriptions and inducing ionic disorder. LYC protects liver against ATR-induced hepatotoxicity, significantly. LYC modulated hepatic ionic homeostasis disturbance via regulation of ATPase activities and their subunits' (1a1, 1b3, 1b4 and 2b4) transcriptions. In summary, these effects play a critical role of LYC-mediated chemoprevention against ATR-induced hepatotoxicity.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 058247368

Download citation: RISBibTeXText

PMID: 26476475

DOI: 10.1016/j.jnutbio.2015.09.009


Related references

Transport stress induces weight loss and heart injury in chicks: disruption of ionic homeostasis via modulating ion transporting ATPases. Oncotarget 8(15): 24142-24153, 2017

Atrazine-induced environmental nephrosis was mitigated by lycopene via modulating nuclear xenobiotic receptors-mediated response. Journal of Nutritional Biochemistry 51: 80-90, 2018

Lycopene protects against atrazine-induced hepatotoxicity through modifications of cytochrome P450 enzyme system in microsomes. Experimental and Toxicologic Pathology 68(4): 223-231, 2016

Role of SH Groups in the Functioning of Ca2+-Transporting ATPases Regulating Ca2+Homeostasis and Exocytosis. Neurophysiology 34(1): 5-12, 2002

Ionic homeostasis disturbance is involved in tomato cell death induced by NaCl and salicylic acid. In Vitro Cellular & Developmental Biology - Plant 48(3): 377-382, 2012

An inhibitor of c-Jun NH2-terminal kinase, SP600125, protects mice from D-galactosamine/lipopolysaccharide-induced hepatic failure by modulating BH3-only proteins. Life Sciences 80(14): 1335-1344, 2007

Heterologous expression of the metal-binding domains of human copper-transporting ATPases (P1-ATPases). Annals of the new York Academy of Sciences 834: 155-157, 1997

Isosteviol Protects Free Fatty Acid- and High Fat Diet-Induced Hepatic Injury via Modulating PKC-β/p66Shc/ROS and Endoplasmic Reticulum Stress Pathways. Antioxidants and Redox Signaling 30(17): 1949-1968, 2019

Tropisetron Protects Against Acetaminophen-Induced Liver Injury via Suppressing Hepatic Oxidative Stress and Modulating the Activation of JNK/ERK MAPK Pathways. Biomed Research International 2016: 1952947, 2016

Dietary supplementation with laminarin, a fermentable marine beta (1-3) glucan, protects against hepatotoxicity induced by LPS in rat by modulating immune response in the hepatic tissue. International Immunopharmacology 7(12): 1497-1506, 2007

Proton-transporting and calcium ion-transporting ATPases of Entamoeba histolytica. Parasitology Today 11(11): 417-420, 1995

Sequence of a Menkes-type Cu-transporting ATPase from rat C6 glioma cells: comparison of the rat protein with other mammalian Cu-transporting ATPases. Molecular and Cellular Biochemistry 181(1-2): 49-61, 1998

The amphiphilic drug-induced tryptophan fluorescence change of ion-transporting ATPases. Biochimica et Biophysica Acta 1188(3): 220-226, 1994

A novel mechanism underlies atrazine toxicity in quails (Coturnix Coturnix coturnix): triggering ionic disorder via disruption of ATPases. Oncotarget 7(51): 83880-83892, 2016

Disturbance of ionic homeostasis in different in vitro models of retinal ischemia. ARVO Annual Meeting Abstract Search & Program Planner : Abstract No 2937, 2003