+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Mesenchymal stem cells: Identification, phenotypic characterization, biological properties and potential for regenerative medicine through biomaterial micro-engineering of their niche



Mesenchymal stem cells: Identification, phenotypic characterization, biological properties and potential for regenerative medicine through biomaterial micro-engineering of their niche



Methods 99: 62-68



Mesenchymal stem cells (MSCs) are multipotent stem cells. Although they were originally identified in bone marrow and described as 'marrow stromal cells', they have since been identified in many other anatomical locations in the body. MSCs can be isolated from bone marrow, adipose tissue, umbilical cord and other tissues but the richest tissue source of MSCs is fat. Since they are adherent to plastic, they may be expanded in vitro. MSCs have a distinct morphology and express a specific set of CD (cluster of differentiation) molecules. The phenotypic pattern for the identification of MSCs cells requires expression of CD73, CD90, and CD105 and lack of CD34, CD45, and HLA-DR antigens. Under appropriate micro-environmental conditions MSCs can proliferate and give rise to other cell types. Therefore, they are ideally suited for the treatment of systemic inflammatory and autoimmune conditions. They have also been implicated as key players in regenerating injured tissue following injury and trauma. MSC populations isolated from adipose tissue may also contain regulatory T (Treg) cells, which have the capacity for modulating the immune system. The immunoregulatory and regenerative properties of MSCs make them ideal for use as therapeutic agents in vivo. In this paper we review the literature on the identification, phenotypic characterization and biological properties of MSCs and discuss their potential for applications in cell therapy and regenerative medicine. We also discuss strategies for biomaterial micro-engineering of the stem cell niche.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 058295220

Download citation: RISBibTeXText

PMID: 26384580

DOI: 10.1016/j.ymeth.2015.09.016


Related references

Mesenchymal stem cells in the umbilical cord: phenotypic characterization, secretome and applications in central nervous system regenerative medicine. Current Stem Cell Research and Therapy 6(3): 221-228, 2011

Mesenchymal Stem Cells in the Umbilical Cord: Phenotypic Characterization, Secretome and Applications in Central Nervous System Regenerative Medicine. Current Stem Cell Research & Therapy 6(3): 221-228, 2011

Characterization and differentiation potential of rabbit mesenchymal stem cells for translational regenerative medicine. In Vitro Cellular and Developmental Biology. Animal 50(3): 251-260, 2014

Fetal mesenchymal stem cells: isolation, properties and potential use in perinatology and regenerative medicine. Bjog 116(2): 166-172, 2009

Morpho-functional characterization of human mesenchymal stem cells from umbilical cord blood for potential uses in regenerative medicine. Stem Cells and Development 18(2): 293-305, 2009

Genetic Engineering of Mesenchymal Stem Cells for Regenerative Medicine. Stem Cells and Development 24(19): 2219-2242, 2015

Mesenchymal stem cells for tissue engineering and regenerative medicine. Biomedical Materials 1(2): 63-71, 2006

Identification, characterization and biological significance of very small embryonic-like stem cells (VSELs) in regenerative medicine. Histology and Histopathology 27(7): 827-833, 2012

Mesenchymal Stromal/Stem Cells in Regenerative Medicine and Tissue Engineering. Stem Cells International 2018: 8031718, 2018

Engineering mesenchymal stem cells for regenerative medicine and drug delivery. Methods 84: 3, 2015

Adult mesenchymal stem cells for tissue engineering versus regenerative medicine. Journal of Cellular Physiology 213(2): 341-347, 2007

Mesenchymal stem cells and progenitor cells in connective tissue engineering and regenerative medicine: is there a future for transplantation?. Langenbeck's Archives of Surgery 396(4): 489-497, 2011

Therapeutic potential of mesenchymal stem cells in regenerative medicine. Stem Cells International 2013: 496218, 2013

Characterization and use of Equine Bone Marrow Mesenchymal Stem Cells in Equine Cartilage Engineering. Study of their Hyaline Cartilage Forming Potential when Cultured under Hypoxia within a Biomaterial in the Presence of BMP-2 and TGF-ß1. Stem Cell Reviews and Reports 13(5): 611-630, 2017

Amniotic membrane and its epithelial and mesenchymal stem cells as an appropriate source for skin tissue engineering and regenerative medicine. Artificial Cells Nanomedicine and Biotechnology 46(Sup2): 431-440, 2018