+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

MicroRNA profiling of antler stem cells in potentiated and dormant states and their potential roles in antler regeneration



MicroRNA profiling of antler stem cells in potentiated and dormant states and their potential roles in antler regeneration



Molecular Genetics and Genomics 291(2): 943-955



MicroRNAs (miRNAs) can effectively regulate gene expression at the post-transcriptional level and play a critical role in tissue growth, development and regeneration. Our previous studies showed that antler regeneration is a stem cell-based process and antler stem cells reside in the periosteum of a pedicle, the permanent bony protuberance, from which antler regeneration takes place. Antlers are the only mammalian organ that can fully regenerate and hence provide a unique opportunity to identify miRNAs that are involved in organ regeneration. In the present study, we used next generation sequencing technology sequenced miRNAs of the stem cells derived from either the potentiated or the dormant pedicle periosteum. A population of both conserved and 20 deer-specific miRNAs was identified. These conserved miRNAs were derived from 453 homologous hairpin precursors across 88 animal species, and were further grouped into 167 miRNA families. Among them, the miR-296 is embryonic stem cell-specific. The potentiation process resulted in the significant regulation (>±2 Fold, q value <0.05) of conserved miRNAs; 8 miRNA transcripts were down- and 6 up-regulated. Several GO biology processes and the Wnt, MAPK and TGF-beta signaling pathways were found to be up-regulated as part of antlerogenic stem cell potentiation process. This research has identified miRNAs that are associated either with the dormant or the potentiated antler stem cells and identified some target miRNAs for further research into their role played in mammalian organ regeneration.

(PDF emailed within 0-6 h: $19.90)

Accession: 058308358

Download citation: RISBibTeXText

PMID: 26738876

DOI: 10.1007/s00438-015-1158-8


Related references

iTRAQ-Based Quantitative Proteomic Analysis of the Potentiated and Dormant Antler Stem Cells. International Journal of Molecular Sciences 17(11): -, 2016

Stem cells responsible for deer antler regeneration are unable to recapitulate the process of first antler development-revealed through intradermal and subcutaneous tissue transplantation. Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution 314(7): 552-570, 2014

Antler regeneration studies with antler removal axial tomography and angiography. Bubenik, G A And A B Bubenik (Ed ) Horns, Pronghorns, And Antlers: Evolution, Morphology, Physiology, And Social Significance Xii+562p Springer-Verlag New York, Inc : New York, New York, Usa; Berlin, West Germany Illus 313-338, 1990

Full-length transcriptome and microRNA sequencing reveal the specific gene-regulation network of velvet antler in sika deer with extremely different velvet antler weight. Molecular Genetics and Genomics 2018, 2018

Biochemical studies on antler cornus cervi parvum part 1 a comparative study on chemical components of antler old antler shark backbone cartilage and whale nasal cartilage. Korean Biochemical Journal 8(2): 89-107, 1975

Stimulation of osteoblastic differentiation and mineralization in MC3T3-E1 cells by antler and fermented antler using Cordyceps militaris. Journal of Ethnopharmacology 133(2): 710-717, 2011

Differential effects of the PI3K/AKT pathway on antler stem cells for generation and regeneration of antlers in vitro. Frontiers in Bioscience 23: 1848-1863, 2019

Quantitative proteomic analysis of deer antler stem cells as a model of mammalian organ regeneration. Journal of Proteomics 2019, 2019

Identification of microRNA-18a as a novel regulator of the insulin-like growth factor-1 in the proliferation and regeneration of deer antler. Biotechnology Letters 36(4): 703-710, 2014

Single-cell transcriptome provides novel insights into antler stem cells, a cell type capable of mammalian organ regeneration. Functional & Integrative Genomics 2019, 2019

Deer antler regeneration: a stem cell-based epimorphic process. Birth Defects Research. Part C, Embryo Today 96(1): 51-62, 2012

Hard fallow deer antler: A living bone till antler casting?. Anatomical Record 255(1): 69-77, May 1, 1999

Recent advances in studies of antler development and neuroendocrine regulation of the antler cycle. Unknown, 1987

The effect of different diets on antler growth during the antler growing period in sika deers. Scientia Agricultura Sinica, 204: 87-92, 1987

Deformation, dewatering, and decollement development in the Antler foreland basin during the Antler Orogeny. Geology (Boulder) 21(11): 1035-1038, 1993