+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Microfluidic Array Chip with Parallel Channels for Fast Preparation of Sample Droplet Array

Microfluidic Array Chip with Parallel Channels for Fast Preparation of Sample Droplet Array

Journal of Nanoscience and Nanotechnology 16(6): 6294-6298

We present a parallel microfluidic array chip for the fast preparation of microdroplets to provide droplet arrays of a sample using the hydrodynamics of immiscible fluids as they flow in a microchamber and bypass channel. The microfluidic chip has eight channels that are parallel from the inlet to the outlet of the device. Each channel has 13 microchambers and bypass channels to trap the droplets from the sample plugs. Because samples can be arrayed simultaneously in eight parallel channels, the arrays of sample droplets can form quickly. After filling the microchannel and chambers with the sample, oil is injected into the device. As the oil fills the microchannel, owing to the difference in the flow resistance between the microchamber and bypass channel, the oil flows through the bypass channel, trapping the sample in the microchamber. A total of 104 microdroplets of an aqueous sample could be successfully trapped in 8 x 13 arrays of microchambers using one simple injection of sequential plugs of mineral oil, the aqueous sample, and mineral oil with surfactant. Sample droplets were successfully formed at all chambers at flow rates of less than 0.06 mL/h, and a trapping efficiency of greater than 90% was achieved at 0.15 mL/h.

(PDF emailed within 0-6 h: $19.90)

Accession: 058313166

Download citation: RISBibTeXText

PMID: 27427705

DOI: 10.1166/jnn.2016.12120

Related references

Parallel separation of multiple samples with negative pressure sample injection on a 3-D microfluidic array chip. Electrophoresis 28(8): 1281-1288, 2007

Droplet Size-Aware and Error-Correcting Sample Preparation Using Micro-Electrode-Dot-Array Digital Microfluidic Biochips. IEEE Transactions on Biomedical Circuits and Systems 11(6): 1380-1391, 2017

A multifunctional microfluidic droplet-array chip for analysis by electrospray ionization mass spectrometry. Lab on A Chip 13(10): 1876-1882, 2013

On-chip analysis, indexing and screening for chemical producing bacteria in a microfluidic static droplet array. Lab on A Chip 16(10): 1909-1916, 2016

Development of micropump-actuated negative pressure pinched injection for parallel electrophoresis on array microfluidic chip. Electrophoresis 30(17): 3053-3057, 2009

High-throughput sample introduction for droplet-based screening with an on-chip integrated sampling probe and slotted-vial array. Lab on A Chip 10(21): 2864-2868, 2010

Improved microfluidic chip-based sequential-injection trapped-droplet array liquid-liquid extraction system for determination of aluminium. Talanta 77(1): 269-272, 2008

A programmable microfluidic static droplet array for droplet generation, transportation, fusion, storage, and retrieval. Lab on A Chip 15(18): 3677-3686, 2016

Fluorescent sensor array in a microfluidic chip. Analytical and Bioanalytical Chemistry 390(1): 307-315, 2007

Microfluidic droplet-array liquid-liquid chromatography based on droplet trapping technique. Lab on A Chip 12(21): 4350-4354, 2013

Luminescent nanomaterials for droplet tracking in a microfluidic trapping array. Analytical and Bioanalytical Chemistry 2018: -, 2018

A rotating microfluidic array chip for staining assays. Talanta 81(4-5): 1203-1208, 2010

Parallel RNA extraction using magnetic beads and a droplet array. Lab on A Chip 15(4): 1059-1065, 2016

Workshop, Cost-Effective and Streamlined Fabrications of Re-Usable World-To-Chip Connectors for Handling Sample of Limited Volume and for Assembling Chip Array. Sensors 18(12), 2018

Optimizing Multiplexed Detections of Diabetes Antibodies via Quantitative Microfluidic Droplet Array. Small: -, 2017