+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Mining expressed sequence tags of rapeseed (Brassica napus L.) to predict the drought responsive regulatory network



Mining expressed sequence tags of rapeseed (Brassica napus L.) to predict the drought responsive regulatory network



Physiology and Molecular Biology of Plants 21(3): 329-340



It is of great significance to understand the regulatory mechanisms by which plants deal with drought stress. Two EST libraries derived from rapeseed (Brassica napus) leaves in non-stressed and drought stress conditions were analyzed in order to obtain the transcriptomic landscape of drought-exposed B. napus plants, and also to identify and characterize significant drought responsive regulatory genes and microRNAs. The functional ontology analysis revealed a substantial shift in the B. napus transcriptome to govern cellular drought responsiveness via different stress-activated mechanisms. The activity of transcription factor and protein kinase modules generally increased in response to drought stress. The 26 regulatory genes consisting of 17 transcription factor genes, eight protein kinase genes and one protein phosphatase gene were identified showing significant alterations in their expressions in response to drought stress. We also found the six microRNAs which were differentially expressed during drought stress supporting the involvement of a post-transcriptional level of regulation for B. napus drought response. The drought responsive regulatory network shed light on the significance of some regulatory components involved in biosynthesis and signaling of various plant hormones (abscisic acid, auxin and brassinosteroids), ubiquitin proteasome system, and signaling through Reactive Oxygen Species (ROS). Our findings suggested a complex and multi-level regulatory system modulating response to drought stress in B. napus.

(PDF emailed within 0-6 h: $19.90)

Accession: 058321408

Download citation: RISBibTeXText

PMID: 26261397

DOI: 10.1007/s12298-015-0311-5


Related references

Expressed sequence tags from the defense of Brassica napus to Leptosphaeria maculans. Molecular Plant Pathology On line: ( 0301 Fristensky), 1999

Identification of drought stress-responsive genes from drought-tolerant groundnut cultivar Arachis hypogaea L cv K-134 through analysis of subtracted expressed sequence tags. Acta Physiologiae Plantarum 34(1): 361-377, 2012

Analysis of expressed sequence tags in Brassica napus cotyledons damaged by crucifer flea beetle feeding. Genome 55(2): 118-133, 2012

Variation between and within species of rapeseed brassica campestris and brassica napus in response to drought stress part 2 growth and development under natural drought stresses. Australian Journal of Agricultural Research 29(3): 479-490, 1978

Development and application of single nucleotide polymorphism markers in the polyploid Brassica napus by 454 sequencing of expressed sequence tags. Plant Breeding 131(2): 293-299, 2012

Generation of expressed sequence tags of random root cDNA clones of Brassica napus by single-run partial sequencing. Plant Physiology 103(2): 359-370, 1993

Differential display-mediated identification of three drought-responsive expressed sequence tags in tea [Camellia sinensis (L.) O. Kuntze. Journal of Biosciences 30(2): 231-235, 2005

Generation and analysis of expressed sequence tags (ESTs) of Camelina sativa to mine drought stress-responsive genes. Biochemical and Biophysical Research Communications 467(1): 83-93, 2016

Genetic analysis of drought stress response in rapeseed brassica campestris and brassica napus 3. physiological characters. Euphytica 28(3): 755-760, 1979

Variation between and within species of rapeseed brassica campestris and brassica napus in response to drought stress part 1 sensitivity at different stages of development. Australian Journal of Agricultural Research 29(3): 469-478, 1978

Variation between and within species of rapeseed brassica campestris and brassica napus in response to drought stress part 3 physiological and physicochemical characters. Australian Journal of Agricultural Research 29(3): 491-502, 1978

The use of physiological and physico chemical parameters as selection criteria in breeding for drought resistance in rapeseed (Brassica campestris and Brassica napus). Plant Breeding Papers Int Congr of the Society for the Advancement of Breeding Researches in Asia and Oceania: d (v 1) 3, 1977

Genetic analysis of drought stress response in rapeseed brassica campestris and brassica napus part 2 yield improvement and the application of selection indices. Euphytica 28(1): 169-177, 1979

Intercropping of indian rapeseed brassica napus var napus and gobhi sarson brassica napus ssp oleifera var annua with different planting patterns. Indian Journal of Agricultural Sciences 59(6): 349-353, 1989

Carbohydrate Dynamics in Leaves of Rapeseed (Brassica napus) Under Drought. Journal of Agronomy and Crop Science 198(3): 207-217, 2012