+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Multiplexed Microsphere Suspension Array-Based Immunoassays

Multiplexed Microsphere Suspension Array-Based Immunoassays

Methods in Molecular Biology 1318: 107-118

ELISA is an extremely powerful tool to detect analytes because of its sensitivity, selectivity, reproducibility and ease of use. Here we describe sandwich immunoassays performed in suspension on spectrally unique microspheres developed by Luminex. Luminex assays offer the benefit of multiplex analysis of large numbers of analytes in a single reaction. Because the microspheres are spectrally unique, many microspheres, each attached to various antibodies, can be added to a single sample. Luminex instruments can distinguish each microsphere and detect the intensity of a reporter signal for each microsphere. Results are reported in Median Fluorescent Intensities for each analyte. Luminex assays can be used to detect up to 500 analytes in a high-throughput format. Luminex refers to this technology as xMAP(®). Here we describe a routine protocol for a Luminex immunoassay. Other Luminex assays would have to be optimized for specific conditions according to their use.

(PDF emailed within 0-6 h: $19.90)

Accession: 058362544

Download citation: RISBibTeXText

PMID: 26160569

DOI: 10.1007/978-1-4939-2742-5_11

Related references

Suspension array with shape-coded silica nanotubes for multiplexed immunoassays. Analytical Chemistry 79(14): 5257-5263, 2007

Multiplexed microsphere-based flow cytometric immunoassays. Current Protocols in Cytometry Chapter 13: Unit13.1-Unit13.1, 2008

Multiplexed Microsphere Suspension-Array Assay for Urine Mitochondrial DNA Typing by C-Stretch Length in Hypervariable Regions. Journal of Clinical Medicine Research 10(7): 552-561, 2018

Multiplexed microsphere-based flow cytometric immunoassays for human cytokines. Journal of Immunological Methods 279(1-2): 277-285, August, 2003

Detection of fumonisin b1 and ochratoxin a in grain products using microsphere-based fluid array immunoassays. Toxins 2(2): 297-309, 2010

Analysis of Plasma Cytokine and Chemokine Profiles in Patients with and without Tuberculosis by Liquid Array-Based Multiplexed Immunoassays. Plos One 11(2): E0148885-E0148885, 2016

Multiplexed magnetic microsphere immunoassays for detection of pathogens in foods. Sensing and Instrumentation for Food Quality and Safety 4(2): 73-81, 2010

A Microsphere-Based Suspension Array for Blood Group Molecular Typing: An Update. Transfusion Medicine and HemoTherapy 37(6): 336-338, 2010

A bead-based suspension array for the multiplexed detection of begomoviruses and their whitefly vectors. Journal of Virological Methods 198: 86-94, 2014

Interference in microsphere flow cytometric multiplexed immunoassays for human cytokine estimation. Cytokine 36(3-4): 180-188, 2007

Development of a multiplexed bead-based suspension array for the detection and discrimination of pospiviroid plant pathogens. Plos One 9(1): E84743-E84743, 2014

Utilizing the Luminex Magnetic Bead-Based Suspension Array for Rapid Multiplexed Phosphoprotein Quantification. Methods in Molecular Biology 1636: 119-131, 2017

Aptamer fluorescence signal recovery screening for multiplex mycotoxins in cereal samples based on photonic crystal microsphere suspension array. Sensors and Actuators B: Chemical 248: 351-358, 2017

TNT detection using multiplexed liquid array displacement immunoassays. Analytical Chemistry 78(7): 2279-2285, 2006

Exploring target-specific primer extension in combination with a bead-based suspension array for multiplexed detection and typing using Streptococcus suis as a model pathogen. Journal of Veterinary Diagnostic Investigation 1040638717730384-1040638717730384, 2017