+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Nanoparticle-Laden Contact Lens for Controlled Ocular Delivery of Prednisolone: Formulation Optimization Using Statistical Experimental Design



Nanoparticle-Laden Contact Lens for Controlled Ocular Delivery of Prednisolone: Formulation Optimization Using Statistical Experimental Design



Pharmaceutics 8(2):



Human eye is one of the most accessible organs in the body, nonetheless, its physiology and associated precorneal factors such as nasolacrimal drainage, blinking, tear film, tear turnover, and induced lacrimation has significantly decreased the residence time of any foreign substances including pharmaceutical dosage forms. Soft contact lenses are promising delivery devices that can sustain the drug release and prolong residence time by acting as a geometric barrier to drug diffusion to tear fluid. This study investigates experimental parameters such as composition of polymer mixtures, stabilizer and the amount of active pharmaceutical ingredient on the preparation of a polymeric drug delivery system for the topical ocular administration of Prednisolone. To achieve this goal, prednisolone-loaded poly (lactic-co-glycolic acid) (PLGA) nanoparticles were prepared by single emulsion solvent evaporation method. Prednisolone was quantified using a validated high performance liquid chromatography (HPLC) method. Nanoparticle size was mostly affected by the amount of co-polymer (PLGA) used whereas drug load was mostly affected by amount of prednisolone (API) used. Longer homogenization time along with higher amount of API yielded the smallest size nanoparticles. The nanoparticles prepared had an average particle size of 347.1 ± 11.9 nm with a polydispersity index of 0.081. The nanoparticles were then incorporated in the contact lens mixture before preparing them. Clear and transparent contact lenses were successfully prepared. When the nanoparticle (NP)-loaded contact lenses were compared with control contact lenses (unloaded NP contact lenses), a decrease in hydration by 2% (31.2% ± 1.25% hydration for the 0.2 g loaded NP contact lenses) and light transmission by 8% (unloaded NP contact lenses 94.5% NP 0.2 g incorporated contact lenses 86.23%). The wettability of the contact lenses remained within the desired value (<90 °C) even upon incorporation of the NP. NP alone and NP-loaded contact lenses both displayed a slow in vitro drug release of drug over 24 h; where 42.3% and 10.8% prednisolone release were achieved, respectively. Contact lenses can be used as a medicated device to sustain ocular drug delivery and improve patient compliance; nonetheless, patients and healthcare professionals' acceptability and perceptions of the new formulations entail further investigations.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 058376808

Download citation: RISBibTeXText

PMID: 27104555

DOI: 10.3390/pharmaceutics8020014


Related references

Formulation and optimization of levofloxacin loaded chitosan nanoparticle for ocular delivery: In-vitro characterization, ocular tolerance and antibacterial activity. International Journal of Biological Macromolecules 108: 650-659, 2018

Formulation and optimization of solid lipid nanoparticle formulation for pulmonary delivery of budesonide using Taguchi and Box-Behnken design. Research in Pharmaceutical Sciences 10(1): 17-33, 2015

Formulation and Optimization of a New Cationic Lipid-Modified PLGA Nanoparticle as Delivery System for Mycobacterium tuberculosis HspX/EsxS Fusion Protein: An Experimental Design. Iranian Journal of Pharmaceutical Research 18(1): 446-458, 2019

Optimization and formulation design of gels of Diclofenac and Curcumin for transdermal drug delivery by Box-Behnken statistical design. Journal of Pharmaceutical Sciences 100(2): 580-593, 2011

Formulation design and optimization of novel soft glycerosomes for enhanced topical delivery of celecoxib and cupferron by Box-Behnken statistical design. Drug Development and Industrial Pharmacy 44(11): 1871-1884, 2018

Development and optimization of solid lipid nanoparticle formulation for ophthalmic delivery of chloramphenicol using a Box-Behnken design. International Journal of Nanomedicine 6: 683-692, 2011

Role of simplex lattice statistical design in the formulation and optimization of microemulsions for transdermal delivery. Biological and Pharmaceutical Bulletin 37(12): 1948-1957, 2014

Role of Simplex Lattice Statistical Design in the Formulation and Optimization of Microemulsions for Transdermal Delivery. Biological & Pharmaceutical Bulletin 37(12): 1948-1957, 2014

Sustained ocular delivery of Dorzolamide-HCl via proniosomal gel formulation: in-vitro characterization, statistical optimization, and in-vivo pharmacodynamic evaluation in rabbits. Drug Delivery 25(1): 1340-1349, 2018

Use of experimental design in the formulation development of a novel contact lens care product. Pharmaceutical Research (New York) 12(9 Suppl. ): S178, 1995

Formulation, characterization and optimization of valsartan self-microemulsifying drug delivery system using statistical design of experiment. Chemical and Pharmaceutical Bulletin 60(11): 1409-1418, 2012

Formulation, Characterization and Optimization of Valsartan Self-Microemulsifying Drug Delivery System Using Statistical Design of Experiment. Chemical & Pharmaceutical Bulletin 60(11): 1409-1418, 2012

Part I: Development and optimization of solid-lipid nanoparticles using Box-Behnken statistical design for ocular delivery of gatifloxacin. Journal of Biomedical Materials Research. Part a 101(6): 1813-1827, 2013

Formulation optimization of gastroretentive drug delivery system for allopurinol using experimental design. Expert Opinion on Drug Delivery 12(4): 513-524, 2015

A stimulus-responsive, in situ-forming, nanoparticle-laden hydrogel for ocular drug delivery. Drug Delivery and Translational Research 8(3): 484-495, 2018