+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Non-Invasive Ultrasound Liver Ablation Using Histotripsy: Chronic Study in an In Vivo Rodent Model



Non-Invasive Ultrasound Liver Ablation Using Histotripsy: Chronic Study in an In Vivo Rodent Model



Ultrasound in Medicine and Biology 42(8): 1890-1902



Hepatocellular carcinoma, or liver cancer, has the fastest growing incidence among cancers in the United States. Current liver ablation methods are thermal-based and share limitations due to the heat sink effect from the blood flow through the highly vascular liver. Recently, our group has investigated histotripsy as a non-invasive liver cancer ablation method. Histotripsy is a non-thermal ultrasonic ablation method that fractionates tissue through the control of acoustic cavitation. Previous experiments in an in vivo porcine model show that histotripsy can create well-confined lesions in the liver through ribcage obstruction without damaging the overlying ribs and other tissues. Histotripsy can also completely fractionate liver tissue surrounding major vessels while preserving the vessels. In this study, we investigate the long-term effects of histotripsy liver ablation in a rodent model. We hypothesize that the fractionated histotripsy lesion will be resorbed by the liver, resulting in effective tissue healing. To test this hypothesis, the livers of 20 healthy rats were treated with histotripsy using an 8-element 1-MHz histotripsy transducer. Rats were euthanized after 0, 3, 7, 14 and 28 days (n = 4). In vivo and post mortem results showed histotripsy lesions were successfully generated through the intact abdomen in all 20 rats. Magnetic resonance imaging found primarily negative contrast on day 0, positive contrast on day 3 and rapid normalization of signal intensity thereafter (i.e., signal amplitude returned to baseline levels seen in healthy liver tissue). Histologically, lesions were completely fractionated into an acellular homogenate. The lesions had a maximum cross-sectional area of 17.2 ± 1.9 mm(2) and sharp boundaries between the lesion and the healthy surrounding tissue after treatment. As the animals recovered after treatment, the histotripsy tissue homogenate was almost completely replaced by regenerated liver parenchyma, resulting in a small fibrous lesion (<1 mm(2) maximum cross-section) remaining after 28 d. The results of this study suggest that histotripsy has potential as a non-invasive liver ablation method for effective tissue removal.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 058413794

Download citation: RISBibTeXText

PMID: 27140521

DOI: 10.1016/j.ultrasmedbio.2016.03.018


Related references

Image-guided non-invasive ultrasound liver ablation using histotripsy: feasibility study in an in vivo porcine model. Ultrasound in Medicine and Biology 39(8): 1398-1409, 2013

Non-Invasive Liver Ablation Using Histotripsy: Preclinical Safety Study in an In Vivo Porcine Model. Ultrasound in Medicine and Biology 43(6): 1237-1251, 2017

Histotripsy: minimally invasive technology for prostatic tissue ablation in an in vivo canine model. Urology 72(3): 682-686, 2008

Histotripsy for Non-Invasive Ablation of Hepatocellular Carcinoma (HCC) Tumor in a Subcutaneous Xenograft Murine Model. Conference Proceedings 2018: 6064-6067, 2018

Developmental impact and lesion maturation of histotripsy-mediated non-invasive tissue ablation in a fetal sheep model. Ultrasound in Medicine and Biology 39(6): 1047-1055, 2013

Mp100-02 Boiling Histotripsy Ablation Of Renal Carcinoma In A Chronic Rat Model. The Journal of Urology 197(4): e1329-e1330, 2017

Ultrasound interstitial mini invasive probes for thermal ablation in liver: feasibility study in vivo. Journal de Radiologie 88(11 Pt 2): 1817-1822, 2007

Morphologic and functional changes in nontumorous liver tissue after radiofrequency ablation in an in vivo model: comparison of 18F-FDG PET/CT, MRI, ultrasound, and CT. Journal of Nuclear Medicine 48(11): 1836-1844, 2007

Non-invasive, Rapid Ablation of Tissue Volume Using Histotripsy. Ultrasound in Medicine and Biology 43(12): 2834-2847, 2017

Microwave ablation energy delivery: influence of power pulsing on ablation results in an ex vivo and in vivo liver model. Medical Physics 41(12): 123301, 2014

Nanodroplet-mediated histotripsy for image-guided targeted ultrasound cell ablation. Theranostics 3(11): 851-864, 2013

Multipolar RFA of the liver: Influence of intrahepatic vessels on ablation zones and appropriateness of CECT in detecting ablation dimensions - Results of an in-vivo porcine liver model. Clinical Hemorheology and Microcirculation 70(4): 467-476, 2018

Pulsed cavitational ultrasound: a noninvasive technology for controlled tissue ablation (histotripsy) in the rabbit kidney. Journal of Urology 175(2): 734-738, 2006

Mp39-14 Boiling Histotripsy Ablation Of In Vivo Renal Carcinoma In The Eker Rat. The Journal of Urology 193(4): e459-e460, 2015

Ultrasound and Contrast-Enhanced Ultrasound for Evaluation of Irreversible Electroporation Ablation: In Vivo Proof of Concept in Normal Porcine Liver. Ultrasound in Medicine and Biology 42(11): 2639-2649, 2016