+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Differential microRNA expression profiles and bioinformatics analysis between young and aging spontaneously hypertensive rats



Differential microRNA expression profiles and bioinformatics analysis between young and aging spontaneously hypertensive rats



International Journal of Molecular Medicine 41(3): 1584-1594



MicroRNAs (miRNAs/miRs) serve a role as important regulators in cardiac hypertrophy. The present study aimed to reveal the differential expression profile of miRNAs between young and aging spontaneously hypertensive rats (SHRs) and studied the functional annotation of predicted targets. Briefly, 3‑month‑old and 12‑month‑old SHRs (n=3/group) were subjected to echocardiography, histopathological analysis and dihydroethidium staining. Subsequently, small RNA sequencing and data processing was conducted to identify the differentially expressed miRNAs between these two groups. Eight significantly upregulated miRNAs were validated by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR), followed by in silico target gene prediction. Functional annotation analysis of the predicted targets was performed using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. As a result, significantly impaired left ventricular diastolic function was detected in the 12‑month‑old SHRs, alongside increased myocyte cross‑sectional area and percentage area of fibrosis, elevated reactive oxygen species production and reduced microvessel density (P<0.05). Compared with the 3‑month‑old SHRs, 21 miRNAs were significantly upregulated and five miRNAs were downregulated in 12‑month‑old rats (P<0.05). Eight upregulated, remodeling‑associated miRNAs, including rno‑miR‑132‑3p, rno‑miR‑182, rno‑miR‑208b‑3p, rno‑miR‑212‑3p, rno‑miR‑214‑3p, rno‑miR‑218a‑5p, rno‑miR‑221‑3p and rno‑miR‑222‑3p, underwent bioinformatics analysis. The target genes were significantly enriched in 688 GO terms and 39 KEGG pathways, including regulation of peptidyl‑tyrosine phosphorylation, regulation of protein serine/threonine kinase activity, adrenergic signaling in cardiomyocytes, ErbB signaling pathway, mTOR signaling pathway, FoxO signaling pathway, Ras signaling pathway, insulin secretion, adipocytokine signaling pathway, HIF‑1 signaling pathway, Rap1 signaling pathway, VEGF signaling pathway and TNF signaling pathway. Collectively, the present study identified a dysregulated miRNA profile in aging SHRs, which targeted numerous signaling pathways associated with cardiac hypertrophy, autophagy, insulin metabolism, angiogenesis and inflammatory response.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 058421962

Download citation: RISBibTeXText

PMID: 29328372

DOI: 10.3892/ijmm.2018.3370


Related references

Altered hippocampal microRNA expression profiles in neonatal rats caused by sevoflurane anesthesia: MicroRNA profiling and bioinformatics target analysis. Experimental and Therapeutic Medicine 12(3): 1299-1310, 2016

Changes in gene expression profiles of left ventricles in aging normotensive and spontaneously hypertensive rats. American Journal of Hypertension 15(4 Part 2): 169A, 2002

Differential gene expression profiles in spontaneously hypertensive rats induced by administration of enalapril and nifedipine. International Journal of Molecular Medicine 31(1): 179-187, 2013

Gene Expression and MicroRNA Expression Analysis in Small Arteries of Spontaneously Hypertensive Rats. Evidence for ER Stress. Plos one 10(9): E0137027, 2015

Altered biogenesis of microRNA-1 is associated with cardiac dysfunction in aging of spontaneously hypertensive rats. Molecular and Cellular Biochemistry 459(1-2): 73-82, 2019

Genetically altered brain amino acid metabolism in spontaneously hypertensive rats: a study by using young spontaneously hypertensive rats and renal hypertensive rats. Clinical and Experimental Hypertension. Part A, Theory and Practice 12(7): 1191-1201, 1990

Analysis of genes causing hypertension and stroke in spontaneously hypertensive rats: gene expression profiles in the brain. International Journal of Molecular Medicine 33(4): 887-896, 2014

Differential expression of nNOS mRNA and protein in the nucleus tractus solitarii of young and aged Wistar-Kyoto and spontaneously hypertensive rats. Journal of Hypertension 23(9): 1683-1690, 2005

Genetic analysis of genes causing hypertension and stroke in spontaneously hypertensive rats: Gene expression profiles in the kidneys. International Journal of Molecular Medicine 36(3): 712-724, 2015

Differential renal gene expression in prehypertensive and hypertensive spontaneously hypertensive rats. American Journal of Physiology. Renal Physiology 289(3): F552-F561, 2005

Aging and microRNA expression in human skeletal muscle: a microarray and bioinformatics analysis. Physiological Genomics 43(10): 595-603, 2011

Downregulation of soluble guanylyl cyclase in young and aging spontaneously hypertensive rats. Circulation Research 85(6): 534-541, 1999

Identification of microRNA-mRNA interactions in atrial fibrillation using microarray expression profiles and bioinformatics analysis. Molecular Medicine Reports 13(6): 4535-4540, 2016

Differential platelet function in stroke prone spontaneously hypertensive rats and spontaneously hypertensive rats. Japanese Heart Journal 23(3): 392, 1982

Expression of atrial natriuretic factor gene in hearts from neonates of spontaneously hypertensive rats and stroke-prone spontaneously hypertensive rats. Journal of Hypertension. Supplement 6(4): S276-S278, 1988