EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Occurrence of Coxiella burnetii and Chlamydiales species in abortions of domestic ruminants and in wild ruminants in Hungary, Central Europe


Journal of Veterinary Diagnostic Investigation 27(2): 206-210
Occurrence of Coxiella burnetii and Chlamydiales species in abortions of domestic ruminants and in wild ruminants in Hungary, Central Europe
Coxiella burnetii and certain members of the Chlamydiales order are zoonotic, intracellular, Gram-negative bacteria, with abortigenic potential in ruminants. These pathogens have a broad host range and worldwide geographical distribution. The current study aimed to reveal the importance of C. burnetii and Chlamydiales spp. in abortions in domestic ruminants and their occurrence in wild ruminants with real-time polymerase chain reaction (qPCR) assays, histology, and immunohistochemical staining (IHC). From the 111 abortion cases of domestic ruminants examined, C. burnetii was detected in 33 placenta samples (cattle, n = 22; sheep, n = 10; goat, n = 1), and members of the Chlamydiales order were detected in 32 placenta samples (cattle, n = 14; sheep, n = 16; goat, n = 2) using qPCR. Coinfection with both C. burnetii and Chlamydiales spp. were identified in 12 cases (cattle, n = 3; sheep, n = 8; goat, n = 1) out of the qPCR-positive samples. The presence of the relevant antigen was confirmed by IHC in 20 cases (C. burnetii, n = 2, in sheep; Chlamydiaceae, n = 17, in sheep [n = 15] and goat [n = 2]; and both pathogens in 1 sheep). Coxiella burnetii was identified in 2.2% (2/91) of the wild ruminants, but the samples were negative by IHC. Uncultured Chlamydiales spp. were detected in 4.4% (4/91) of the placenta samples by qPCR. In conclusion, Q fever is widespread among domestic ruminants in Hungary, and, in several cases, C. burnetii was implicated as the primary cause of abortions. Waddlia chondrophila, Parachlamydia spp., and uncultured Chlamydiales spp. were present only sporadically in samples from cattle and wild ruminants.

(PDF same-day service: $19.90)

Accession: 058448585

PMID: 25776545

DOI: 10.1177/1040638714563566



Related references

Genotyping of Coxiella burnetii from domestic ruminants and human in Hungary: indication of various genotypes. Bmc Veterinary Research 10(): 107-107, 2015

Evaluation of diagnosis techniques used for non-brucellosis spontaneous abortions in Ruminants. Identification of Chlamydia spp., Coxiella burnetii et Toxoplasma gondii in Deux-Sevres and Vienne on a series of 150 cattle, sheep and goat abortions. Rencontres Autour des Recherches sur les Ruminants 11: 317-320, 2004

Coxiella burnetii DNA detected in domestic ruminants and wildlife from Portugal. Veterinary Microbiology 180(1-2): 136-141, 2016

Genotyping of Coxiella burnetii from domestic ruminants in northern Spain. Bmc Veterinary Research 8: 241-241, 2013

Coxiella burnetii Infections in domestic ruminants in Canary Islands (Spain). Transboundary and Emerging Diseases 57(6): 464-464, 2011

Prevalence of Coxiella burnetii infection in domestic ruminants: a critical review. Veterinary Microbiology 149(1-2): 1-16, 2011

Seroprevalence of Coxiella burnetii in selected populations of domestic ruminants in Newfoundland. Canadian Veterinary Journal 43(5): 363-364, 2002

Diagnosis by PCR of Coxiella burnetii in aborted fetuses of domestic ruminants in Portugal. Veterinary Record 164(12): 373-374, 2009

Coxiella burnetii in humans, domestic ruminants, and ticks in rural western Kenya. American Journal of Tropical Medicine and Hygiene 88(3): 513-518, 2013

Seroprevalence of Coxiella burnetii in domestic ruminants in Gran Canaria Island, Spain. Transboundary and Emerging Diseases 57(1-2): 66-67, 2010