+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Occurrence of Cryptosporidium spp. and Giardia spp. in a public water-treatment system, Paraná, Southern Brazil



Occurrence of Cryptosporidium spp. and Giardia spp. in a public water-treatment system, Paraná, Southern Brazil



Revista Brasileira de Parasitologia Veterinaria 24(3): 303-308



The purpose of this study was to investigate the occurrence of Cryptosporidium spp. and Giardia spp. in a public water-treatment system. Samples of raw and treated water were collected and concentrated using the membrane filtration technique. Direct Immunofluorescence Test was performed on the samples. DNA extraction using a commercial kit was performed and the DNA extracted was submitted to a nested-PCR reaction (n-PCR) and sequencing. In the immunofluorescence, 2/24 (8.33%) samples of raw water were positive for Giardia spp.. In n-PCR and sequencing, 2/24 (8.33%) samples of raw water were positive for Giardia spp., and 2/24 (8.33%) samples were positive for Cryptosporidium spp.. The sequencing showed Cryptosporidium parvum and Giardia duodenalis DNA. In raw water, there was moderate correlation among turbidity, color and Cryptosporidium spp. and between turbidity and Giardia spp.. The presence of these protozoans in the water indicates the need for monitoring for water-treatment companies.

(PDF emailed within 0-6 h: $19.90)

Accession: 058448588

Download citation: RISBibTeXText

PMID: 26291147

DOI: 10.1590/S1984-29612015051


Related references

Giardia sp. cysts and Cryptosporidium spp. oocysts dynamics in southeast Brazil: occurrence in surface water and removal in water treatment processes. Water Science and Technology: Water Supply 4(2): 15-22, 2004

Occurrence of oocysts of Cryptosporidium spp. and cysts of Giardia spp. at different stages of water treatment, in Campinas, Sao Paulo, Brazil. Higiene Alimentar 18(118): 52-59, 2004

Investigation of Cryptosporidium spp. and Giardia spp. in a public water-treatment system. Zoonoses and Public Health 56(5): 221-228, 2008

WATER FROM DIFFERENT SOURCES USED FOR THE IRRIGATION OF VEGETABLES TO BE MARKETED: RESEARCH ON Cryptosporidium spp., Giardia spp., AND COLIFORMS IN PARANA, BRAZIL. Revista do Instituto de Medicina Tropical de Sao Paulo 57(4): 333-336, 2016

Occurrence of Cryptosporidium oocysts and Giardia cysts in raw water from the Atibaia River, Campinas, Brazil. Revista do Instituto de Medicina Tropical de Sao Paulo 43(2): 109-111, 2001

Assessing the infection risk of Giardia and Cryptosporidium in public drinking water delivered by surface water systems in Sao Paulo State, Brazil. Science of the Total Environment 442: 389-396, 2013

Occurrence and removal of Giardia spp. cysts and Cryptosporidium spp. oocysts from a municipal wastewater treatment plant in Brazil. Environmental Technology 38(10): 1245-1254, 2016

Occurrence of Giardia cysts and Cryptosporidium oocysts in raw and treated water from two water treatment plants in Selangor, Malaysia. Water Research 31(12): 3132-3136, 1997

Occurrence of Cryptosporidium oocysts and Giardia cysts in the Nakdong River and their removal during water treatment. Journal of Water and Health 5(1): 163-169, 2007

Polymerase chain reaction and nested-PCR approaches for detecting Cryptosporidium in water catchments of water treatment plants in Curitiba, State of Paraná, Brazil. Revista Da Sociedade Brasileira de Medicina Tropical 46(3): 270-276, 2014

Occurrence of Cryptosporidium, Giardia, and Cyclospora in influent and effluent water at wastewater treatment plants in Arizona. Science of the Total Environment 484: 129-136, 2015

Morphometry and cysts counts of Giardia per gram of feces CPG in ovines in Cidade Gaucha - Parana - Brazil Morfometria e cistos por grama de fezes CPG de Giardia em ovinos de Cidade Gaucha - Parana - Brazil. Revista UNIMAR. Agosto; 162: 281-287, 1994

Comparing the efficacy of chlorine, chlorine dioxide, and ozone in the inactivation of Cryptosporidium parvum in water from Parana State, Southern Brazil. Applied Biochemistry and Biotechnology 151(2-3): 464-473, 2008

Morphometry and cysts counting of Giardia per gram of feces CPG in dairy cattle in northwest of Parana - Brazil Morfometria e contagem de cistos por grama de fezes CPG de Giardia em bovinos de leite no noroeste do Parana - Brasil. Revista UNIMAR. Setembro; 183: 607-615, 1996

Techniques for detecting Giardia and Cryptosporidium in water samples a review Revision de tecnicas para detectar Giardia y Cryptosporidium en muestras de agua. Boletin del Centro de Investigaciones Biologicas Universidad del Zulia, 323: 213-244, 1998