+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn

+ Translate
+ Recently Requested

Olive Recombinant Hydroperoxide Lyase, an Efficient Biocatalyst for Synthesis of Green Leaf Volatiles



Olive Recombinant Hydroperoxide Lyase, an Efficient Biocatalyst for Synthesis of Green Leaf Volatiles



Applied Biochemistry and Biotechnology 179(4): 671-683



Volatile C6-aldehydes are the main contributors to the characteristic odor of plants known as "green note" and are widely used by the flavor industry. Biotechnological processes were developed to fulfill the high demand in C6-aldehydes in natural flavorants and odorants. Recombinant hydroperoxide lyases (HPLs) constitute an interesting alternative to overcome drawbacks arising from the use of HPL from plant extracts. Thus, olive recombinant 13-HPL was assayed as biocatalysts to produce C6-aldehydes. Firstly, a cDNA encoding for olive HPL of Leccino variety was isolated and cloned in pQE-30 expression vector. In order to improve the enzyme solubility, its chloroplast transit peptide was deleted. Both enzymes (HPL wild type and HPL deleted) were expressed into Escherichia coli strain M15, purified, characterized, and then used for bioconversion of 13-hydroperoxides of linoleic and linolenic acids. Aldehydes produced were extracted, then identified and quantified using gas chromatography and mass spectrometry. Recombinant HPL wild type (HPLwt) allowed producing 5.61 mM of hexanal and 4.39 mM of 3Z-hexenal, corresponding to high conversion yields of 93.5 and 73 %, respectively. Using HPL deleted (HPLdel) instead of HPLwt failed to obtain greater quantities of hexanal or 3Z-hexenal. No undesirable products were formed, and no isomerization of 3Z-hexenal in 2E-hexenal occurred. The olive recombinant HPLwt appears to be a promising efficient biocatalyst for the production of C6-aldehydes.

(PDF emailed within 0-6 h: $19.90)

Accession: 058452597

Download citation: RISBibTeXText

PMID: 26961190

DOI: 10.1007/s12010-016-2023-x


Related references

Green leaf volatiles: hydroperoxide lyase pathway of oxylipin metabolism. Current Opinion in Plant Biology 9(3): 274-280, 2006

CYP74B24 is the 13-hydroperoxide lyase involved in biosynthesis of green leaf volatiles in tea (Camellia sinensis). Plant Physiology and Biochemistry 98: 112-118, 2016

Enzymatic synthesis of green notes with hydroperoxide-lyase from olive leaves and alcohol-dehydrogenase from yeast in liquid/gas reactor. Process Biochemistry 44(10): 1122-1127, 2009

C6-volatiles in homogenates from green leaves: localization of hydroperoxide lyase activity. Lebensmittel Wissenschaft Technologie = Food science technology9(2): 152-155, 1986

6 carbon volatiles in homogenates from green leaves localization of hydroperoxide lyase activity. Lebensmittel-Wissenschaft & Technologie 19(2): 152-155, 1986

A Tea Hydroperoxide Lyase Gene, CsiHPL1, Regulates Tomato Defense Response Against Prodenia Litura (Fabricius) and Alternaria Alternata f. sp Lycopersici by Modulating Green Leaf Volatiles (GLVs) Release and Jasmonic Acid (JA) Gene Expression. Plant Molecular Biology Reporter 32(1): 62-69, 2014

Activation and Stabilization of Olive Recombinant 13-Hydroperoxide Lyase Using Selected Additives. Applied Biochemistry and Biotechnology 182(3): 1000-1013, 2016

A simple and efficient system for green note compound biogenesis by use of certain lipoxygenase and hydroperoxide lyase sources. Journal of Agricultural and Food Chemistry 53(17): 6877-6882, 2005

Transcriptome analysis of Arabidopsis thaliana treated with green leaf volatiles: possible role of green leaf volatiles as self-made damage-associated molecular patterns. Journal of Pesticide Science 43(3): 207-213, 2018

Thermal stability of lipoxygenase and hydroperoxide lyase from olive fruit and repercussion on olive oil aroma biosynthesis. Journal of Agricultural and Food Chemistry 55(15): 6309-6313, 2007

Inactivation of tea leaf hydroperoxide lyase by fatty acid hydroperoxide. Journal of Agricultural & Food Chemistry 40(2): 175-178, 1992

Isolation, expression, and characterization of a 13-hydroperoxide lyase gene from olive fruit related to the biosynthesis of the main virgin olive oil aroma compounds. Journal of Agricultural and Food Chemistry 58(9): 5649-5657, 2010

Simple, Efficient, and Green Method for Synthesis of Trisubstituted Electrophilic Alkenes Using Lipase as a Biocatalyst. Synthetic Communications 42(3): 412-423, 2012

Synthesis of green note aroma compounds by biotransformation of fatty acids using yeast cells coexpressing lipoxygenase and hydroperoxide lyase. Applied Microbiology and Biotechnology 93(1): 159-168, 2012

Hydroperoxide lyase from olive (Olea europaea) fruits. Plant Science Limerick 143(1): 19-26, 1999