+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Optimization of multimodal spectral imaging for assessment of resection margins during Mohs micrographic surgery for basal cell carcinoma



Optimization of multimodal spectral imaging for assessment of resection margins during Mohs micrographic surgery for basal cell carcinoma



Biomedical Optics Express 6(1): 98-111



Multimodal spectral imaging (MSI) based on auto-fluorescence imaging and Raman micro-spectroscopy was used to detect basal cell carcinoma (BCC) in tissue specimens excised during Mohs micrographic surgery. In this study, the MSI algorithm was optimized to maximize the diagnosis accuracy while minimizing the number of Raman spectra: the segmentation of the auto-fluorescence images was optimized according to the type of BCC, sampling points for Raman spectroscopy were generated based on auto-fluorescence intensity variance and segment area, additional Raman spectra were acquired when performance of the segmentation algorithm was sub-optimal. The results indicate that accurate diagnosis can be achieved with a sampling density of ~2,000 Raman spectra/cm(2), based on sampling points generated by the MSI algorithms. The key benefit of MSI is that diagnosis of BCC is obtained based on intrinsic chemical contrast of the tissue, within time scales similar to frozen-section histopathology, but without requiring laborious sample preparation and subjective interpretation of stained frozen-sections.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 058468510

Download citation: RISBibTeXText

PMID: 25657878

DOI: 10.1364/boe.6.000098


Related references

Test Characteristics of High-Resolution Ultrasound in the Preoperative Assessment of Margins of Basal Cell and Squamous Cell Carcinoma in Patients Undergoing Mohs Micrographic Surgery. Dermatologic Surgery 35(1): 9-16, 2009

Test characteristics of high-resolution ultrasound in the preoperative assessment of margins of basal cell and squamous cell carcinoma in patients undergoing Mohs micrographic surgery. Dermatologic Surgery 35(1): 9, 2009

Inserting ex vivo fluorescence confocal microscopy perioperatively in Mohs micrographic surgery expedites bedside assessment of excision margins in recurrent basal cell carcinoma. Dermatology 227(1): 89-92, 2013

The efficacy of curettage in delineating margins of basal cell carcinoma before Mohs micrographic surgery. Dermatologic Surgery 29(9): 899-903, 2003

Photodynamic Therapy Followed by Mohs Micrographic Surgery Compared to Mohs Micrographic Surgery Alone for the Treatment of Basal Cell Carcinoma: Results of a Pilot Single-Blinded Randomised Controlled Trial. Journal of Cutaneous and Aesthetic Surgery 8(2): 88-91, 2015

Management of periocular basal cell carcinoma Mohs micrographic surgery versus radiotherapy I Mohs micrographic surgery. Survey of Ophthalmology 38(2): 193-203, 1993

Use of a perimeter technique with Mohs micrographic surgery in the resection of a giant basal cell carcinoma. Journal of Cutaneous Medicine and Surgery 16(6): 465-468, 2012

Use of a Perimeter Technique with Mohs Micrographic Surgery in the Resection of a Giant Basal Cell Carcinoma. Journal of Cutaneous Medicine and Surgery 16(6): 465-468, 2012

Optical coherence tomography-based optimization of mohs micrographic surgery of Basal cell carcinoma: a pilot study. Dermatologic Surgery 39(4): 627-633, 2013

Subclinical infiltration of Basal cell carcinoma in asian patients: assessment after mohs micrographic surgery. Annals of Dermatology 23(3): 276-281, 2011

Mohs micrographic surgery for repeat excision of basal cell carcinomas on the head with positive margins. Actas Dermo-Sifiliograficas 102(10): 797-804, 2011

Bispectral fluorescence imaging of aggressive basal cell carcinoma combined with histopathological mapping: a preliminary study indicating a possible adjunct to Mohs micrographic surgery. British Journal of Dermatology 154(2): 305-309, 2006

Mohs' micrographic surgery for basal cell carcinoma. Clinical and Experimental Dermatology 24(2): 130-133, 1999

Mohs micrographic surgery for Basal cell carcinoma of the face. Archives of Dermatology 145(12): 1428-1430, 2009

Mohs micrographic surgery for the basal cell carcinoma treatment. Virchows Archiv 439(3): 367, 2001