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Oxaliplatin mediated inhibition of human hepatocellular cancer cell proliferation is via the regulation of signal transduction pathways



Oxaliplatin mediated inhibition of human hepatocellular cancer cell proliferation is via the regulation of signal transduction pathways



Journal of Clinical Oncology 23(16_suppl): 4270-4270



NlmCategory="UNASSIGNED">4270 Oxaliplatin has been shown to have efficacy in colorectal cancer both in the in vitro and in vivo setting. In this particular study, the preclinical assessment of Oxaliplatin in treating HCC was further explored in two human hepatocellular carcinoma cell lines, HEP3B and HEPG2. The IC 50 was achieved at approximately 1μM in HEPG2 and 2.5μM in HEP3B. Using flow cytometry, we demonstrated accumulation of cells in the SubG1 phase which is consistent with apoptosis. After demonstrating these phenotypic changes, to further explore the mechanism related with cytotoxicity, the cell cycle regulatory proteins were examined. Cyclin A, D, E expression as detected by western blot decreased after treatment with oxaliplatin. Expression of Hypoxia induced Factor (HIF) was similarly inhibited, along with the signal transducer and activator of transcription factor (STAT-1) and phosphorylated Akt. It was unclear as to the effect of oxaliplatin on STAT-3 and ERK expression. Through its ability to regulate signal transduction pathways, specifically cell cycle and apoptosis, oxaliplatin is able to exert its cytotoxic effects in HCC. No significant financial relationships to disclose.

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Accession: 058488008

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PMID: 27945045


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