+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Oxidant effects and toxicity of Croton campestris in Drosophila melanogaster



Oxidant effects and toxicity of Croton campestris in Drosophila melanogaster



Pharmaceutical Biology 54(12): 3068-3077



Croton campestris A.St.-Hil. (Euphorbiaceae) is a species native to Northeast Brazil used by traditional communities for the treatment of a variety of health problems. However, potential toxicological effects of this plant are unknown. The potential toxicity of the hydroalcoholic extract of C. campestris leaves on Drosophila melanogaster insect model, additionally with phytochemical constitution and cellular mechanisms mediating the action of extract were analysed in this study. Constituents of the extract were evaluated by HPLC. In vitro antioxidant potential of extract was analysed by DPPH, ABTS and FRAP. Flies injected culture medium mixed with extract (0.1-50 mg/mL) for 72 h. After, ROS production was evaluated by DCF-DA oxidation. Phosphorylation of MAPK signalling pathway was investigated by Western blotting method. Activity of antioxidant enzymes was analysed in homogenates. Major components of the extract include quercetin (38.11 ± 0.06 mg/g), caffeic acid (20.06 ± 0.17 mg/g) and kaempferol (15.45 ± 0.05 mg/g). Consumption of the extract impaired locomotor performance and induced fly death of flies (LC50 of 26.51 mg/mL). Augmented ROS formation and SOD, CAT and GST activity were observed from 0.1 mg/mL. JNK and p38 kinases phosphorylation was modulated and Paraquat-induced toxicity was augmented by extract. Our data show important toxicological effects of C. campestris leading to increased mortality and impaired locomotor performance accompanied by induction of cell stress markers in flies. The study draws attention to the indiscriminate use of plant extracts.

(PDF emailed within 0-6 h: $19.90)

Accession: 058488147

Download citation: RISBibTeXText

PMID: 27417881

DOI: 10.1080/13880209.2016.1207089


Related references

Comparative effects of environmental ethanol on Drosophila melanogaster and Drosophila simulans adults, including geographic differences in Drosophila melanogaster. Genetic studies of Drosophila populations: proceedings of Kioloa conference edited by JB Gibson and JG Oakeshott: 7, 1981

Effects of cadmium on drosophila melanogaster toxicity proteins and transfer rna. Toxicology & Applied Pharmacology 60(2): 368-378, 1981

Acute and chronic toxicity effects of silver nanoparticles (NPs) on Drosophila melanogaster. Environmental Science and Technology 45(11): 4974-4979, 2011

Protective effects of alpha-ketoglutarate against aluminum toxicity in Drosophila melanogaster. Comparative Biochemistry and Physiology. Toxicology and Pharmacology 217: 41-53, 2018

Genetic effects of ethylene dibromide in Drosophila melanogaster Air pollutants, toxicity. Environmental science research5(25): 291-300, 1982

Effects of mycotoxin pretreatment on aflatoxin B1 post-treatment toxicity in Drosophila melanogaster (Diptera). Journal of Invertebrate Pathology 41(3): 321-327, 1983

Evaluation of toxicity and genotoxic effects of spinosad and deltamethrin in Drosophila melanogaster and Bactrocera oleae. Pest Management Science 67(12): 1534-1540, 2012

Effects of differential alcohol dehydrogenase activity on the developmental toxicity of ethanol in Drosophila melanogaster. Teratology 36(3): 329-334, 1987

Relationships within the drosophila melanogaster species subgroup of the genus drosophila sophophora part 1 inversion polymorphisms in drosophila melanogaster and drosophila simulans. Proceedings of the Royal Society of London Series B Biological Sciences 193(1111): 137-157, 1976

Differential effects of arsenite and arsenate to Drosophila melanogaster in a combined adult/developmental toxicity assay. Bulletin of Environmental Contamination and Toxicology 42(2): 276-282, 1989

Toxicity of coumarins synthesized by Pechmann-Duisberg condensation against Drosophila melanogaster larvae and antibacterial effects. Food and Chemical Toxicology 109(Pt 2): 1118-1124, 2017

The protective effects of Rhodiola crenulata extracts on Drosophila melanogaster gut immunity induced by bacteria and SDS toxicity. PhytoTherapy Research 28(12): 1861-1866, 2015

Antimicrobial peptides increase tolerance to oxidant stress in Drosophila melanogaster. Journal of Biological Chemistry 286(8): 6211-6218, 2011

Lengthening of the average life of drosophila melanogaster by the addition of anti oxidant compounds to the culture medium. Revista Sociedad Mexicana De Historia Natural: 1-214, 1972

Label-free distribution of anti-amyloid D-AIP in Drosophila melanogaster: prevention of Ab42-induced toxicity without side effects in transgenic flies. Journal of Neurochemistry 2019, 2019