+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Oxidative damage and histopathological changes in lung of rat chronically exposed to nicotine alone or associated to ethanol

Oxidative damage and histopathological changes in lung of rat chronically exposed to nicotine alone or associated to ethanol

Pathologie-Biologie 63(6): 258-267

Smoking is the most important preventable risk factor of chronic obstructive pulmonary disease and lung cancer. This study was designed to investigate oxidative damage and histopathological changes in lung tissue of rats chronically exposed to nicotine alone or supplemented with ethanol. Twenty-four male Wistar rats divided into three groups were used for the study. The nicotine group received nicotine (2.5mg/kg/day); the nicotine-ethanol group was given simultaneously same dose of nicotine plus ethanol (0.2g/kg/day), while the control group was administered only normal saline (1 ml/kg/day). The treatment was administered by subcutaneous injection once daily for a period of 18 weeks. Chronic nicotine administration alone or combined to ethanol caused a significant increase in malondialdehyde (MDA) level, superoxide dismutase (SOD) activity and catalase (CAT) activity in lung tissue compared to control rats suggesting an oxidative damage. However, these increases were mostly prominent in nicotine group. The histopathological examination of lung tissue of rats in both treated groups revealed many alterations in the pulmonary structures such as emphysema change (disappearance of the alveolar septa, increased irregularity and size of air sacs) and marked lymphocytic infiltration in perivascular and interstitial areas. However, the changes characterized in the nicotine group (pulmonary congestion, hemorrhage into alveoli and interstitial areas, edema) were more drastic than those observed in the nicotine-ethanol group, and they can be attributed to a significant degree of capillary endothelial permeability and microvascular leak. Conversely, the ethanol supplementation caused an appearance of fatty change and fibrosis in pulmonary tissue essentially due to a metabolism of ethanol. Finally, the lung damage illustrated in nicotine group was more severe than that observed in the nicotine-ethanol group. We conclude that the combined administration of nicotine and ethanol may moderate the effect of nicotine administered independently by counteractive interactions between these two drugs.

(PDF emailed within 0-6 h: $19.90)

Accession: 058488714

Download citation: RISBibTeXText

PMID: 26586280

DOI: 10.1016/j.patbio.2015.10.001

Related references

Changes in endocannabinoid contents in the brain of rats chronically exposed to nicotine, ethanol or cocaine. Brain Research 954(1): 73-81, 1 November, 2002

Oxidative damage in substantia nigra and striatum of rats chronically exposed to ozone. Journal of Chemical Neuroanatomy 31(2): 114-123, 2005

Taurine Ameliorates Renal Oxidative Damage and Thyroid Dysfunction in Rats Chronically Exposed to Fluoride. Biological Trace Element Research (): -, 2016

Mitochondrial oxidative damage and myocardial fibrosis in rats chronically intoxicated with moderate doses of ethanol. Toxicology Letters (Shannon) 123(2-3): 209-216, September 15th, 2001

Oxidative damage, biochemical and histopathological alterations in rats exposed to chlorpyrifos and the antioxidant role of zinc. Pesticide Biochemistry And Physiology: 1, 14-23, 2010

Oxidative stress, progressive damage in the substantia nigra and plasma dopamine oxidation, in rats chronically exposed to ozone. Toxicology Letters 197(3): 193-200, 2010

Oxidative damage and antioxidant status in the lungs and bronchoalveolar lavage fluid of rats exposed chronically to cigarette smoke. Journal of Biochemical Toxicology 10(1): 11-17, 1995

Close association of intestinal autofluorescence with the formation of severe oxidative damage in intestine of nematodes chronically exposed to Al(2)O(3)-nanoparticle. Environmental Toxicology and Pharmacology 32(2): 233-241, 2012

Oxidative damage to proteins and DNA in rats exposed to cadmium and/or ethanol. Chemico-Biological Interactions 180(1): 31-38, 2009

Acquisition of nicotine discrimination and discriminative stimulus effects of nicotine in rats chronically exposed to caffeine. Journal of Pharmacology and Experimental Therapeutics 288(3): 1053-1073, 1999

Oxidative stress and mitochondrial damage precedes gastric cell death exposed to ethanol. Gastroenterology 112(4 SUPPL ): A149, 1997

Histopathological, oxidative damage, biochemical, and genotoxicity alterations in hepatic rats exposed to deltamethrin: modulatory effects of garlic (Allium sativum). Canadian Journal of Physiology and Pharmacology 94(6): 571-578, 2016

Dimethoate induced oxidative damage and histopathological changes in lung of adult rats: modulatory effects of selenium and/or vitamin E. Biomedical and Environmental Sciences 25(3): 340-351, 2012

Evaluation of Direct-Oxidative DNA Damage on Human Lung Epithelial Cells Exposed to Urban Airborne Particulate Matter. Water, Air & Soil Pollution: Focus 9(1-2): 69-77, 2009

Association between oxidative DNA damage and the expression of 8-oxoguanine DNA glycosylase 1 in lung epithelial cells of neonatal rats exposed to hyperoxia. Molecular Medicine Reports 11(6): 4079-4086, 2015