+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Pixel Grafting: An Evolution of Mincing for Transplantation of Full-Thickness Wounds

Pixel Grafting: An Evolution of Mincing for Transplantation of Full-Thickness Wounds

Plastic and Reconstructive Surgery 137(1): 92e-99e

Split-thickness skin grafting is the gold standard for treatment of major skin loss. This technique is limited by donor-site availability in large burn injuries. With micrografting, a technique where split-thickness skin graft is minced into 0.8 × 0.8-mm pieces, the authors have demonstrated an expansion ratio of 1:100 and healing comparable to that achieved with split-thickness skin grafting. In this study, the authors explore the regenerative potential of a skin graft by cutting split-thickness skin grafts to pixel size (0.3 × 0.3 mm) grafts. Wound healing was studied in full-thickness wounds in a porcine model by creating an incubator-like microenvironment using polyurethane wound chambers. Multiple wound healing parameters were used to study the outcome of pixel grafting and compare it to micrografting and nontransplanted wounds. The authors' results show that 0.3 × 0.3-mm pixel grafts remain viable and contribute to skin regeneration. The pixel graft-transplanted wounds demonstrated a faster reepithelialization rate, decreased wound contraction, and increased mechanical stability compared with nontransplanted wounds. The reepithelialization rates of the wounds were significantly increased with pixel grafting at day 6 after wounding compared with micrografting. Among the other wound healing parameters, there were no significant differences between wounds transplanted with pixel grafts and micrografts. Pixel grafting technique would address the most commonly encountered limitations of the split-thickness skin graft with the possibility of an even larger expansion ratio than micrografting. This technique is simple and fast and can be conducted in the operating room or in the clinic.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 058559526

Download citation: RISBibTeXText

PMID: 26710065

DOI: 10.1097/prs.0000000000001871

Related references

Keratinocyte grafting: a new means of transplantation for full-thickness wounds. Journal of Dermatologic Surgery and Oncology 14(1): 75-78, 1988

Keratinocyte Grafting: A New Means of Transplantation for Full-Thickness Wounds. The Journal of Dermatologic Surgery and Oncology 14(1): 75-78, 1988

Dermal substitutes for full-thickness wounds in a one-stage grafting model. Wound Repair and Regeneration 1(4): 244-252, 1993

Significance of dermis for grafting cultured keratinocytes on full thickness wounds. British Journal of Dermatology 127(4): 425-426, 1992

Expeditious care of full-thickness burn wounds by surgical excision and grafting. Annals of Surgery 125(1): 1-22, 2018

Experiences with the surgical excision and skin grafting of extensive full thickness burn wounds. Harlem Hospital Bulletin 2(4): 159-173, 1950

Development of the mechanical properties of engineered skin substitutes after grafting to full-thickness wounds. Journal of Biomechanical Engineering 136(5): 051008, 2015

Full-thickness skin grafting to cover equine wounds caused by laceration or tumor resection. Veterinary Surgery 39(6): 708-714, 2010

Identification of Merkel cells associated with neurons in engineered skin substitutes after grafting to full thickness wounds. Plos One 14(3): E0213325, 2019

Dual grafting of autologous epidermal cells and skin allografts in the full-thickness burn wounds. Zhonghua Zheng Xing Shao Shang Wai Ke Za Zhi 4(1): 25-26, 1988

An animal model to study the significance of dermis for grafting cultured keratinocytes on full thickness wounds. Burns. 20(Suppl. 1): S57-S60, 1994

Primary grafting research of tissue engineered oral mucosa lamina propria on skin full thickness wounds. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 20(2): 172-176, 2006

Accelerated epithelialization and improved wound healing metrics in porcine full-thickness wounds transplanted with full-thickness skin micrografts. Wound Repair and Regeneration 25(5): 816-827, 2017

Hyperpigmentation after surgery for a deep dermal burn of the dorsum of the hand: partial-thickness debridement followed by medium split-thickness skin grafting vs full-thickness debridement followed by thick split-thickness skin grafting. Burns and Trauma 4: 9, 2016

Skin regenerated from cultured epithelial autografts on full-thickness burn wounds from 6 days to 5 years after grafting. A light, electron microscopic and immunohistochemical study. Laboratory Investigation; a Journal of Technical Methods and Pathology 60(5): 600-612, 1989