+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Poly(A) binding protein 1 enhances cap-independent translation initiation of neurovirulence factor from avian herpesvirus



Poly(A) binding protein 1 enhances cap-independent translation initiation of neurovirulence factor from avian herpesvirus



Plos One 9(12): E114466



Poly(A) binding protein 1 (PABP1) plays a central role in mRNA translation and stability and is a target by many viruses in diverse manners. We report a novel viral translational control strategy involving the recruitment of PABP1 to the 5' leader internal ribosome entry site (5L IRES) of an immediate-early (IE) bicistronic mRNA that encodes the neurovirulence protein (pp14) from the avian herpesvirus Marek's disease virus serotype 1 (MDV1). We provide evidence for the interaction between an internal poly(A) sequence within the 5L IRES and PABP1 which may occur concomitantly with the recruitment of PABP1 to the poly(A) tail. RNA interference and reverse genetic mutagenesis results show that a subset of virally encoded-microRNAs (miRNAs) targets the inhibitor of PABP1, known as paip2, and therefore plays an indirect role in PABP1 recruitment strategy by increasing the available pool of active PABP1. We propose a model that may offer a mechanistic explanation for the cap-independent enhancement of the activity of the 5L IRES by recruitment of a bona fide initiation protein to the 5' end of the message and that is, from the affinity binding data, still compatible with the formation of 'closed loop' structure of mRNA.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 058569131

Download citation: RISBibTeXText

PMID: 25503397

DOI: 10.1371/journal.pone.0114466


Related references

Cellular stress in Xenopus kidney cells enhances the phosphorylation of eukaryotic translation initiation factor 4E and the association of eIF4F with poly -binding protein. Biochemical Journal 342(3): 519-526, Sept 15, 1999

Cellular stress in xenopus kidney cells enhances the phosphorylation of eukaryotic translation initiation factor (eIF)4E and the association of eIF4F with poly(A)-binding protein. Biochemical Journal 342 Pt 3: 519-526, 1999

Poly(A)-binding protein-interacting protein 1 binds to eukaryotic translation initiation factor 3 to stimulate translation. Molecular and Cellular Biology 28(21): 6658-6667, 2008

Translation initiation on mRNAs bound by nuclear cap-binding protein complex CBP80/20 requires interaction between CBP80/20-dependent translation initiation factor and eukaryotic translation initiation factor 3g. Journal of Biological Chemistry 287(22): 18500-9, 2012

Interference with interaction between eukaryotic translation initiation factor 4G and poly(A)-binding protein in Xenopus oocytes leads to inhibition of polyadenylated mRNA translation and oocyte maturation. Journal of Biochemistry 130(6): 737-740, 2001

Translation initiation factor 4B homodimerization, RNA binding, and interaction with Poly(A)-binding protein are enhanced by zinc. Journal of Biological Chemistry 283(52): 36140-36153, 2008

Eukaryotic initiation factor 4G-poly(A) binding protein interaction is required for poly(A) tail-mediated stimulation of picornavirus internal ribosome entry segment-driven translation but not for X-mediated stimulation of hepatitis C virus translation. Molecular and Cellular Biology 21(13): 4097-4109, 2001

Competitive and noncompetitive binding of eIF4B, eIF4A, and the poly(A) binding protein to wheat translation initiation factor eIFiso4G. Biochemistry 49(38): 8251-8265, 2010

Translational silencing of ceruloplasmin requires the essential elements of mRNA circularization: poly(A) tail, poly(A)-binding protein, and eukaryotic translation initiation factor 4G. Molecular and Cellular Biology 21(19): 6440-6449, 2001

Association of the yeast poly(A) tail binding protein with translation initiation factor eIF-4G. Embo Journal 15(24): 7168-7177, 1996

Wheat germ poly binding protein enhances the binding affinity of eukaryotic initiation factor 4F and 4F for cap analogues. Biochemistry 37(7): 1910-1916, Feb 17, 1998