+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Prediction of the antiglycation activity of polysaccharides from Benincasa hispida using a response surface methodology



Prediction of the antiglycation activity of polysaccharides from Benincasa hispida using a response surface methodology



Carbohydrate Polymers 151: 358-363



Benincasa hispida is a popular vegetable in China. Our previous experiments suggested that polysaccharides isolated from B. hispida fruits (PBH) have antiglycation effect and DPPH free radical scavenging activity. Ultrasonic treatments can be used to extract polysaccharides from Benincasa hispida (PBH). The aim of this study was to investigate the correlation between the ultrasonic treatment conditions and the antiglycation activity of PBH. A mathematical model was generated with an artificial neural network (ANN) toolbox from MATLAB to analyze the effects of ultrasonic treatment conditions on antiglycation activity. The response surface plots showed relationships between ultrasonic extraction conditions and bioactivity. The R(2) value of the model was 0.9919, which suggested good fitness of the neural network. The application of genetic algorithms showed that the optimal ultrasonic extraction conditions resulted in the highest antiglycation activity for PBH. These were 150W, 46°C, and 35min. These conditions produced a predicted antiglycation activity of 41.2%; the actual activity was 40.9% under optimal conditions. This is very close to the predicted value. The experimental data indicated that the PBH possessed both antiglycation and antioxidant activities. The maximum actual value of antiglycation was 101.7% that of the positive control, and the PBH inhibited the DPPH free radicals with an EC50 value of 0.98mg/mL. This is 66.2% that of ascorbic acid. These results explained the observations that B. hispida can decrease glucose levels in diabetic patients. The experimental results also showed that the ANN could be used for optimization and prediction.

(PDF emailed within 0-6 h: $19.90)

Accession: 058596310

Download citation: RISBibTeXText

PMID: 27474577

DOI: 10.1016/j.carbpol.2016.05.079


Related references

Optimization of ultrasound-assisted extraction of crude oil from winter melon (Benincasa hispida) seed using response surface methodology and evaluation of its antioxidant activity, total phenolic content and fatty acid composition. Molecules 17(10): 11748-11762, 2013

Cell wall polysaccharides from chalkumra (Benincasa hispida) fruit. I. Isolation and characterization of pectins. Journal of agricultural and food chemistry 2 52(11): 3556-3562, 2004

Cell wall polysaccharides from chalkumra (Benincasa hispida) fruit. Part I. Isolation and characterization of pectins. Journal of Agricultural and Food Chemistry 52(11): 3556-3562, 2004

Structural characterisation of hemicellulosic polysaccharides from Benincasa hispida using specific enzyme hydrolysis, ion exchange chromatography and MALDI-TOF mass spectroscopy. Carbohydrate Polymers 59(2): 231-238, 2005

Antioxidant activity of benincasa hispida seeds. 2012

Optimization of hydroxyl radical scavenging activity of exo-polysaccharides from Inonotus obliquus in submerged fermentation using response surface methodology. Journal of Microbiology and Biotechnology 20(4): 835-843, 2010

Nootropic activity of methanol extract of Benincasa hispida fruit. Indian Journal of Pharmacology 35(2): 130, April, 2003

Anti-convulsant activity of Benincasa hispida fruit, methanol extract. Journal of Natural Remedies 4(2): 195-198, 2004

Investigation of anxiolytic-like effect of antidepressant activity of Benincasa hispida, methanol extract. Indian Journal of Pharmacology 35(2): 129-130, April, 2003

Antidepressant-like activity of Benincasa hispida fruits in mice: Possible involvement of monoaminergic and GABAergic systems. Journal of Pharmacology and Pharmacotherapeutics 3(1): 60-62, 2012

Antinociceptive and anti-pyretic activity of Benincasa hispida (thunb.) cogn. in Wistar albino rats. Pakistan Journal of Pharmaceutical Sciences 22(3): 287-290, 2009

Dose dependent activity of Benincasa hispida on colchicine induced experimental rat model of Alzheimers disease. Ijp: 4, 237-244, 2008

Determination of the presence of ribosome inactivating protein activity in selected medicinal plants and purification of a new RIP from Benincasa hispida. Plant Biology (Rockville) : 65, 1998

Supercritical carbon dioxide extraction of seed oil from winter melon (Benincasa hispida) and its antioxidant activity and fatty acid composition. Molecules 18(1): 997-1014, 2013

Purification, antioxidant activity and antiglycation of polysaccharides from Polygonum multiflorum Thunb. Carbohydrate Polymers 99: 765-773, 2014