+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Preparation, characterization and application of a reversed phase liquid chromatography/hydrophilic interaction chromatography mixed-mode C18-DTT stationary phase



Preparation, characterization and application of a reversed phase liquid chromatography/hydrophilic interaction chromatography mixed-mode C18-DTT stationary phase



Talanta 146: 442-451



A mixed-mode chromatographic stationary phase, C18-DTT (dithiothreitol) silica (SiO2) was prepared through "thiol-ene" click chemistry. The obtained material was characterized by fourier transform infrared spectroscope, nitrogen adsorption analysis and contact angle analysis. Chromatographic performance of the C18-DTT was systemically evaluated by studying the effect of acetonitrile content, pH, buffer concentration of the mobile phase and column temperature. It was demonstrated that the novel stationary phase possessed reversed phase liquid chromatography (RPLC)/hydrophilic interaction liquid chromatography (HILIC) mixed-mode property. The stop-flow test revealed that C18-DTT exhibited excellent compatibility with 100% aqueous mobile phase. Additionally, the stability and column-to-column reproducibility of the C18-DTT material were satisfactory, with relative standard deviations of retention factor of the tested analytes (verapamil, fenbufen, guanine, tetrandrine and nicotinic acid) in the range of 1.82-3.72% and 0.85-1.93%, respectively. Finally, the application of C18-DTT column was demonstrated in the separation of non-steroidal anti-inflammatory drugs, aromatic carboxylic acids, alkaloids, nucleo-analytes and polycyclic aromatic hydrocarbons. It had great resolving power in the analysis of various compounds in HILIC and RPLC chromatographic conditions and was a promising RPLC/HILIC mixed-mode stationary phase.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 058609090

Download citation: RISBibTeXText

PMID: 26695288

DOI: 10.1016/j.talanta.2015.09.009


Related references

Preparation of a Monolithic Column with Mixed-Mode Stationary Phase of Reversed-Phase/Hydrophilic-Interaction for Capillary Liquid Chromatography. Journal of Separation Science 2018, 2018

Synthesis, characterization, and application of a novel multifunctional stationary phase for hydrophilic interaction/reversed phase mixed-mode chromatography. Talanta 174: 703-714, 2018

Preparation and characterization of surfactin-modified silica stationary phase for reversed-phase and hydrophilic interaction liquid chromatography. Journal of Chromatography. a 1371: 257-260, 2015

Preparation of pH-responsive stationary phase for reversed-phase liquid chromatography and hydrophilic interaction chromatography. Journal of Chromatography. a 1212(1-2): 61-67, 2008

Preparation and chromatographic evaluation of a newly designed steviol glycoside modified-silica stationary phase in hydrophilic interaction liquid chromatography and reversed phase liquid chromatography. Journal of Chromatography. a 1388: 110-118, 2015

Chromatographic evaluation of a newly designed peptide-silica stationary phase in reverse phase liquid chromatography and hydrophilic interaction liquid chromatography: mixed mode behavior. Journal of Chromatography. a 1266: 43-52, 2013

Imidazolium embedded C8 based stationary phase for simultaneous reversed-phase/hydrophilic interaction mixed-mode chromatography. Journal of Chromatography. a 1400: 107-116, 2015

Core-Shell Metal-Organic Frameworks As The Mixed-Mode Stationary Phase For Hydrophilic Interaction/Reversed-Phase Chromatography. Acs Applied Materials and Interfaces 2019, 2019

Imidazolium-embedded iodoacetamide-functionalized silica-based stationary phase for hydrophilic interaction/reversed-phase mixed-mode chromatography. Journal of Separation Science 39(18): 3498-3504, 2016

Novel imidazolium-embedded N,N-dimethylaminopropyl-functionalized silica-based stationary phase for hydrophilic interaction/reversed-phase mixed-mode chromatography. Analytical and Bioanalytical Chemistry 407(30): 8989-8997, 2016

New poly(ionic liquid)-grafted silica multi-mode stationary phase for anion-exchange/reversed-phase/hydrophilic interaction liquid chromatography. Analyst 137(11): 2553-2555, 2012

Mixed mode hydrophilic and ionic interaction chromatography rivals reversed phase liquid chromatography for the separation of peptides. Journal of Chromatography 594(1-2): 75-86, 1992

Tetra-proline modified calix[4]arene bonded silica stationary phase for simultaneous reversed-phase/hydrophilic interaction mixed-mode chromatography. Journal of Separation Science 2019, 2019

Preparation and characterization of vinylimidazole-based polymer monolithic stationary phases for reversed-phase and hydrophilic interaction capillary liquid chromatography. Talanta 187: 73-82, 2018

Preparation of a mixed-mode hydrophilic interaction/anion-exchange polymeric monolithic stationary phase for capillary liquid chromatography of polar analytes. Journal of Chromatography. a 1216(5): 801-806, 2009