+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Preparative isolation and purification of xanthohumol from hops (Humulus lupulus L.) by high-speed counter-current chromatography



Preparative isolation and purification of xanthohumol from hops (Humulus lupulus L.) by high-speed counter-current chromatography



Food Chemistry 132(1): 619-623



Xanthohumol (XN) and related prenylflavonoids are the main bioactive components of hops (Humulus lupulus L.). The current work is to investigate the use of high-speed counter-current chromatography (HSCCC) in search for high isolation of xanthohumol from hops. A solvent system consisted of n-hexane-ethyl acetate-methanol-water at a volume ratio of 5:5:4:3 was employed. The results demonstrated that the constructed method could be well applied for the isolation of xanthohumol from hops extract. After HSCCC isolation procedure, the purity of xanthohumol was over 95% assayed by HPLC and the yield of extraction was 93.60%. The chemical structure identification of xanthohumol was carried out by UV, (1)H NMR and (13)C NMR. The present results demonstrated that xanthohumol could be efficiently obtained using a single HSCCC step from H. lupulus L. extract.

(PDF emailed within 0-6 h: $19.90)

Accession: 058609251

Download citation: RISBibTeXText

PMID: 26434340

DOI: 10.1016/j.foodchem.2011.10.098


Related references

Preparative isolation and purification of four compounds from Cistanches deserticola Y.C. Ma by high-speed counter-current chromatography. Molecules 17(7): 8276-8284, 2012

Preparative isolation and purification of astaxanthin from the microalga Chlorococcum sp. by high-speed counter-current chromatography. Journal of Chromatography. A 925(1-2): 133-137, 2001

Preparative isolation and purification of psoralen and isopsoralen from Psoralea corylifolia by high-speed counter-current chromatography. Journal of Chromatography, A 1057(1/2): 225-228, 2004

Preparative isolation and purification of polymethoxylated flavones from Tangerine peel using high-speed counter-current chromatography. Journal of Chromatography, A 1090(1/2): 188-192, 2005

Preparative isolation and purification of indigo and indirubin from Folium isatidis by high-speed counter-current chromatography. Phytochemical Analysis 23(6): 637-641, 2013

Preparative isolation and purification of four flavonoids from the petals of Nelumbo nucifera by high-speed counter-current chromatography. Phytochemical Analysis 21(3): 268-272, 2010

Preparative isolation and purification of coumarins from Cnidium monnieri (L.) Cusson by high-speed counter-current chromatography. Journal of Chromatography, A 1055(1/2): 71-76, 2004

Preparative isolation and purification of coumarins from Peucedanum praeruptorum Dunn by high-speed counter-current chromatography. Journal of Chromatography, A 1057(1/2): 89-94, 2004

Preparative isolation and purification of anthocyanins from purple sweet potato by high-speed counter-current chromatography. Journal of Separation Science 32(12): 2146-2151, 2009

Preparative isolation and purification of two amides from Mallotus lianus Croiz by high-speed counter-current chromatography. Journal of Separation Science 31(22): 3930-3935, 2009

Preparative isolation and purification of harpagoside from Scrophularia ningpoensis hemsley by high-speed counter-current chromatography. Phytochemical Analysis 17(6): 406-408, 2006