+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Pro-inflammatory caspase-1 activation during the immune response in cells from rainbow trout Oncorhynchus mykiss (Walbaum 1792) challenged with pathogen-associated molecular patterns



Pro-inflammatory caspase-1 activation during the immune response in cells from rainbow trout Oncorhynchus mykiss (Walbaum 1792) challenged with pathogen-associated molecular patterns



Journal of Fish Diseases 38(11): 993-1003



In response to pathogens, the higher vertebrate innate immune system activates pro-inflammatory caspase-1 which is responsible for the processing and secretion of several important cytokines involved in the host's defence against infection. To date, caspase-1 has been described in few teleost fish, and its activity has been demonstrated through substrate cleavage and inhibition by pharmacological agents. In this study, the detection of the active form of caspase-1 during the immune response in salmonid fish is described, where two antibodies were produced. These antibodies differentially recognize the structural epitopes of the inactive pro-caspase-1 and the processed active form of the caspase. Firstly, caspase-1 activation was demonstrated in vitro by ELISA, Western blotting and immunocytochemistry in rainbow trout macrophages exposed to different pathogen-associated molecular patterns plus the pathogen Aeromonas hydrophila. This activity was clearly abrogated by a caspase inhibitor and seems to be unrelated to IL-1β secretion. Caspase-1 activation was then validated in vivo in gill cells from fish challenged with Aeromonas salmonicida. These results represent the first demonstration of caspase-1 activation in salmonids, and the first evidence of the putative regulatory role which this protease plays in inflammatory response in this fish group, as described for some other teleosts and mammals.

(PDF emailed within 0-6 h: $19.90)

Accession: 058627889

Download citation: RISBibTeXText

PMID: 25477241

DOI: 10.1111/jfd.12315


Related references

Examinations of rainbow trout Oncorhynchus mykiss Walbaum, 1792 with using ultrasonography Ultrasonografi ile Gokkusagi Alabaliginin Oncorhynchus mykiss Walbaum, 1792 incelenmesi. Su Urunleri Dergisi, 201-2: 211-216, 2003

Inflammatory response of rainbow trout Oncorhynchus mykiss (Walbaum, 1792) larvae against Ichthyophthirius multifiliis. Fish and Shellfish Immunology 34(2): 521-528, 2013

The Effect of Kefir as a Dietary Supplement on Nonspecific Immune Response and Disease Resistance in Juvenile Rainbow Trout, Oncorhynchus mykiss (Walbaum 1792). Journal of the World Aquaculture Society 48(2): 248-256, 2017

Sequential development of the immune response in rainbow trout oncorhynchus mykiss walbaum 1792 to experimental plerocercoid infections of diphyllobothrium dendriticum nitzsch 1824. Parasitology 104(1): 169-178, 1992

Sequential development of the immune response in rainbow trout [Oncorhynchus mykiss (Walbaum, 1792)] to experimental plerocercoid infections of Diphyllobothrium dendriticum (Nitzsch, 1824). Parasitology 104 Pt 1: 169-178, 1992

Molecular characterization of rainbow trout,Oncorhynchus mykiss(Walbaum, 1792) stocks in India. Journal of Genetics 94(2 Supplement): 13-18, 2015

The relationship and the fecundity between the egg size and the body weight of rainbow trout Oncorhynchus mykiss Walbaum, 1792 and brook trout Salvelinus fontinalis Mitchil, 1814 Gokkusagi Alabaligi Oncorhynchus mykiss Walbaum, 1792 ve Kaynak Alabaligi Salvelinus fontinalis Mitchil, 1814nin Yumurta Capi ile Vucut Buyuklugu Arasindaki Iliski ve Yumurta Verimleri. Su Urunleri Dergisi. 2005( ) Temmuz-Aralik; 223-4: 435-438, 2006

In vitro evaluation of gut contractile response to histamine in rainbow trout (Oncorhynchus mykiss Walbaum, 1792). Research in Veterinary Science 84(1): 126-131, 2007

Phenotypic and molecular characterization of Yersinia ruckeri isolates from rainbow trout (Oncorhynchus mykiss, Walbaum, 1792) in Turkey. Berliner und Munchener Tierarztliche Wochenschrift 124(7-8): 320-328, 2011

Acute toxicity of m-cresol on the fry rainbow trout (Oncorhynchus mykiss Walbaum, 1792). Fresenius Environmental Bulletin 14(6): 536-538, 2005

Immune response of rainbow trout (Oncorhynchus mykiss, Walbaum) to Aeromonas hydrophila. Fish and Shellfish Immunology 4(4): 239-254, 1994

Morphological parameters of the vertebral column in the rainbow trout Oncorhynchus mykiss Teleostei, Salmoniform Descripteurs morphologiques de la zonation de la colonne vertebrale chez la truite arc-en-ciel Oncorhynchus mykiss Walbaum, 1792 Teleostei, Salmoniforme. Annales des Sciences Naturelles Zoologie et Biologie Animale. juillet-septembre; 203: 87-97, 1999

Growth of rainbow trout oncorhynchus mykiss walbaum 1792 related to egg size and temperature. Dana 9: 31-38, 1991

Occurence of rainbow trout Oncorhynchus mykiss Walbaum 1792 Pisces Salmonidae in the upper Aiuruoca River and tributaries, Grande river basin, Minas Gerais, Brazil Ocorrencia de trata arco-iris Oncorhynchus mykiss Walbaum, 1792 Pisces Salmonidae no alto rio Aiuruoca e tributarios, bacia do rio Grande, Minas Gerais, Brasil. Boletim do Museu de Biologia Mello Leitao. Dezembro; 14: 33-40, 2002

Normal developmental stages and levels of organogenesis of the rainbow trout Oncorhynchus mykiss (Walbaum, 1792). Zeitschrift fuer Fischkunde, 32: 127-204, 1996