+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Proteomic Analyses of Purified Particles of the Rabies Virus

Proteomic Analyses of Purified Particles of the Rabies Virus

Bing du Xue Bao 31(3): 209-216

The rabies virus (RABV) is an enveloped RNA virus. It mainly damages the central nervous system and causes anencephaly in mammals and humans. There is now compelling evidence that enveloped virions released from infected cells can carry many host proteins, some of which may play an important part in viral replication. Several host proteins have been reported to be incorporated into RABV particles. However, a systematic study to reveal the proteomics of RABV particles has not been conducted. In the present study, after virus culture and purification by sucrose density gradient ultracentrifugation, a proteomics approach was used to analyze the protein composition of purified RABV particles to understand the molecular mechanisms of virus-cell interactions. Fifty host proteins, along with five virus-encoded structural proteins, were identified in purified RABV particles. These proteins could be classified into ten categories according to function: intracellular trafficking (14%), molecular chaperone (12%), cytoskeletal (24%), signal transduction (8%), transcription regulation (12%), calcium ion-binding (6%), enzyme binding (6%), metabolic process (2%), ubiquitin (2%) and other (14%). Of these, four proteins (beta-actin, p-tubulin, Cofilin, Hsc70) were validated by western blotting to be present in purified RABV particles. This novel study of the composition of host proteins in RABV particles may aid investigation of the mechanism of RABV replication.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 058652702

Download citation: RISBibTeXText

PMID: 26470524

Related references

Proteomic analysis of purified Newcastle disease virus particles. Proteome Science 10(1): 32, 2012

Proteomic analysis of purified Newcastle disease virus particles. Proteome Science 10(1): 32, 2012

Heterogeneity in the phospholipid content of purified rabies virus (ERA strain) particles. Journal of General Virology 16(2): 173-183, 1972

Proteomic analysis of purified coronavirus infectious bronchitis virus particles. Proteome Science 8: 29, 2010

A Proteomic-Based Workflow Using Purified Respiratory Syncytial Virus Particles to Identify Cellular Factors as Drug Targets. Methods in Molecular Biology 1442: 175-194, 2016

Identification of rabies virus mimotopes screened from a phage display peptide library with purified dog anti-rabies virus serum IgG. Virus Research 174(1-2): 47-51, 2013

Chimeric rabies virus-like particles containing membrane-anchored GM-CSF enhances the immune response against rabies virus. Viruses 7(3): 1134-1152, 2015

Mokola virus glycoprotein and chimeric proteins can replace rabies virus glycoprotein in the rescue of infectious defective rabies virus particles. Journal of Virology 69(3): 1444-1451, 1995

Study on the preparation of purified PHKC rabies vaccine with GHKC-adapted rabies virus strain. Journal of Microbiology 21(1): 24-26, 2001

Evaluation of the safety, immunogenicity, and pharmacokinetic profile of a new, highly purified, heat-treated equine rabies immunoglobulin, administered either alone or in association with a purified, Vero-cell rabies vaccine. Acta Tropica. 70(3): 317-333, Y 30, 1998

Human diploid cell culture rabies vaccine (HDCV) and purified chick embryo cell culture rabies vaccine (PCECV) both confer protective immunity against infection with the silver-haired bat rabies virus strain (SHBRV). Vaccine 16(17): 1656-1659, 1998

Live vaccinia-rabies virus recombinants, but not an inactivated rabies virus cell culture vaccine, protect B-lymphocyte-deficient A/WySnJ mice against rabies: considerations of recombinant defective poxviruses for rabies immunization of immunocompromised individuals. Vaccine 22(25/26): 3329-3333, 2004

Protecting Indian schoolchildren against rabies: pre-exposure vaccination with purified chick embryo cell vaccine (PCECV) or purified verocell rabies vaccine (PVRV). Human Vaccines 4(5): 365-369, 2008

Purified chick embryo cell rabies vaccine and human rabies immune globulin in post exposure protection against rabies. Kuwert, E Et Al (Ed ) Rabies in The Tropics; International Conference, Tunis, North Africa, Oct 3-6, 1983 Xviii+786p Springer-Verlag: Berlin, West Germany; New York, N Y , Usa Illus 133-137, 1985

Rabies virus antinucleoprotein antibody protects against rabies virus challenge in vivo and inhibits rabies virus replication in vitro. Journal of Virology 67(10): 6080-6086, 1993